ESTIV Congress > Accepted Posters

Accepted Posters

1. Bio-engineering, stem cells and disease models 

P-1-1

The effect of methylmercury chloride on three germ layer formation during hiPSC-derived Embryoid Bodies development

Justyna Augustyniak1, Hanna Kozlowska2, Agnieszka Stasinska1, Leonora BuzanskaP1
1 Department of Stem Cells Bioengineering, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland
2 Laboratory of Advanced Microscopy Techniques, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
P Presenting author

P-1-2

Preliminary evaluation of extracted collagen from food fish-processing side streams as an active ingredient for cosmetic application.

Lorenzo DonderoP1, Giulia De Negri Atanasio1, Federica Robino2, Anastasiia Kharina2, Micaela Tiso3, Francesca Rispo1, Giorgia Allaria1, Francesca Tardanico1, Federica Turrini4, Federica Grasso4, Valentina Orlandi4, Matteo Zanotti Russo2, Raffaella Boggia4, Elena Grasselli1
1  Department of Earth, Environment and Life Science, University of Genoa, Genova, Italy
2 ANGEL CONSULTING S.a.s. , Italy
3 MICAMO Spin-Off Department of Earth Sciences, University of Genoa, Genova, Italy
4 Department of Pharmacy, University of Genoa, Genova, Italy
P Presenting author

P-1-3

An innovative and robust strategy to generate hepatocyte-like cells from individual patients to investigate idiosyncratic hepatotoxicity of drugs

GUILLEM GARCIA LLORENSP1,2, TERESA MARTÍNEZ SENA1, JOSE VICENTE CASTELL RIPOLL1,2,3, ROQUE BORT MARTI1,3
1 Experimental Hepatology Research Unit, Instituto de Investigación Sanitaria La Fe
2  Biochemistry and Molecular Biology Department, Universidad de Valencia, Valencia, Spain.
3 CIBEREHD
P Presenting author

P-1-4

Human peripheral neurons with enhanced nociceptor features for the study of pain-related dysfunctions

Anna-Katharina HolzerP1, Christiaan Karreman1, Ilinca Suciu1, Harald Wohlfarth1, Marcel Leist1
1 In vitro Toxicology and Biomedicine, Dept inaugurated by the Doerenkamp-Zbinden foundation at the University of Konstanz, Germany
P Presenting author

P-1-5

Development of in vitro cardiotoxicity assessment using human iPS cell technology

Yasunari KandaP1, Sae Hayashi1, Ayano Satsuka1
1 National Institute of Health Sciences (NIHS) Japan
P Presenting author

P-1-6

Stem cell toxicity is detected by cell internal indicator, SERPINB2

Seong-Kwan KimP1,2, Se-Ra Park1,2, Soo-Rim Kim1,2, In-Sun Hong1,2
1 Laboratory of stem cell research, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 406-840, Republic of Korea.
2 Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 406-840, Republic of Korea.
P Presenting author

P-1-7

Establishment of an in vitro rabbit neurosphere model  to evaluate neurodevelopmental adverse effects induced by Intra-uterine growth restriction (IUGR) and to test future therapies

Britta Anna KühneP1,2, Paula Vázquez1,2, Carla Loreiro2, Fatima Crispi2, Eduard Gratacós2, Jesús Gómez-Catalán1, Ellen  Fritsche3, Miriam Illa2, Marta Barenys1,2
1 GRET, INSA‐UB and Toxicology Unit, Pharmacology and Therapeutical Chemistry Department, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
2 BCNatal-Barcelona Center for Maternal-Fetal and Neonatal Medicine (Hospital Clínic and Hospital Sant Joan de Déu), Fetal i+D Fetal Medicine Research Center, IDIBAPS, Barcelona, Spain.
3 IUF – Leibniz Research Institute for Environmental Medicine. Auf’m Hennekamp 50, 40225 Düsseldorf, Germany.
P Presenting author

P-1-8

Generation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes using the Aurora Kinase Inhibitor ZM447439

Su-Jin LeeP1, Hyang-Ae Lee1
1 Korea Institute of Toxicology
P Presenting author

P-1-9

Mechanism of the Cyclic Stretch Induced Maturation of the Human Induced Pluripotent Stem Cell-derived Cardiomyocytes

Hyang-Ae LeeP1, Su-Jin Lee1, Hyeon-A Kim1
1 Korea Institute of Toxicology
P Presenting author

P-1-10

Cellular Model of Parkinson`s Disease for Safety Testing of Selenium-Based Nanodelivery System

Ivan MamićP1, Maja Beus2, Nikolina Kalčec2, Nikolina Peranić2, Petra Turčić1, Ivana Vinković Vrček2
1 Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, Zagreb, Croatia
2 Institute for Medical Research and Occupational Health, Ksaverska cesta 2, Zagreb, Croatia
P Presenting author

P-1-11

Study the toxicity effect of Cycloporin A on angiogenesis progress of endothelial cells derived from induced pluripotent stem cells

Zahra MazidiP1, Matthias  Wieser1, Regina  Grillari2, Johannes Grillari3
1 Evercyte GmbH
2 Evercyte GmbH, Correspondence author
3 Ludwig Boltzmann Institiute, Evecyte GbmH, Correspondence Author
P Presenting author

P-1-12

Discovery of a novel function, immune-modulatory factor CSF-2, improves the therapeutic effect of stem cells from cell damage

Se-Ra ParkP1,2, Seong-Kwan  Kim1,2, Soo-Rim Kim1,2, In-Sun  Hong1,2
1 Laboratory of stem cell research, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 406-840, Republic of Korea.
2 Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 406-840, Republic of Korea.
P Presenting author

P-1-13

Studying melanoma progression on a commensal 3D-skin model

Aline RosinP1, Maya Kissner1, Tewes Tralau2, Lisa  Lemoine1
1 German Federal Institute for Risk Assessment Department Pesticides Safety, Junior Research Group Skin Microbiome
2 German Federal Institute for Risk Assessment Department Pesticides Safety
P Presenting author

P-1-14

A novel in vitro 3D model of chronic kidney disease (CKD) in the proximal tubule for drug development and safety.

Elena TasinatoP1,2, Kathryn Garner2, Lyle Armstrong1,2, Colin D A Brown2
1 Newcastle University
2 Newcells Biotech
P Presenting author

2a. Models, biomarkers and assays for endocrine disruption and developmental toxicity 

P-2a-1

Chlorpyrifos impairs immortalized hypothalamic murine GnRH neurons at human relevant exposure levels

Gabriele Lori1,2, Maria Luisa Casella3, Antonella Tinari1, Sabrina TaitP1
1 Center for Gender-Specific medicine, Istituto Superiore di Sanità
2 Science Department, Università Degli Studi di Roma Tre
3 Core Facilities, Istituto Superiore di Sanità
P Presenting author

P-2a-2

Evaluation of the local tolerance of flagellin aerosol therapy (FLAMOD) on primary human cell-based 3D in vitro nasal, bronchial, small-airway and alveolar models

Jean-Claude Sirard1, Itziar Sanjuan1, Charlotte Green-Jensen2, Nathalie Heuze Vourc’h3, Edouard Sage 4, Cindia FerreiraP5, Samuel Constant5
1 Institut Pasteur de Lille – Inserm, France
2 Statens Serum Institut
3 Université de Tours, France
4 Hopital Foch, France
5 Epithelix
P Presenting author

P-2a-3

Investigation on the reproducibility of the DIO1 inhibition in vitro method based on human liver microsomes

Andreas-Georg Weber1, Barbara Birk1, Hans-Albrecht Huener1, Kostja Renko2, Sandra CoeckeP3, Steffen Schneider1, Bennard Van Ravenzwaay1, Robert Landsiedel3
1 BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany
2 2Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Berlin, Germany
3 European Commission, Joint Research Centre (JRC), Ispra, Italy
P Presenting author

P-2a-4

A closer look at the evaluation criteria of the H295R In vitro Steroidogenesis Assay test guideline

Caroline Gomes1, Claudia Ruelker1, Nina HambruchP1, Tilmann Walk2, Michael Herold2, Naveed  Honarvar1, Hennicke Kamp2, Dorothee Funk-Weyer1, Robert Landsiedel1
1 BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany
2 BASF Metabolome Solutions GmbH, Berlin, Germany
P Presenting author

P-2a-5

LC-MS based analysis of substance-induced displacement of thyroid hormone from its serum binding protein

Andreas Weber1, Lars-Henrik Koeppl1, Melanie Weißenfeld1, Nina HambruchP1, Barbara Birk1, Eric Fabian1, Steffen Schneider1, Stephanie Melching-Kollmuss2, Dorothee Funk-Weyer1, Robert Landsiedel1
1 BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany
2 BASF SE, Agriculture Solutions, Global Toxicology, Limburgerhof, Germany
P Presenting author

P-2a-6

Impact of interference with retinoid signaling on in vitro differentiation in human neural stem cell-based model

Barbara  Kubickova 1, Sarka Martinkova1, Ondrej Brozman1, Klara HilscherovaP1
1 RECETOX, Faculty of Science, Masaryk University
P Presenting author

P-2a-7

In Vitro pharmacologic profiling For Cosmetic Chemical Systemic Toxicity Safety Testing: A case study on Homosalate

Matthew BurbankP1, Nicola Jane Hewitt2, Gerry Kenna2, Mareike Boettcher3, Catherine Mahony4, Johanna Ebmeyer3, Duncan Armstrong5, Audrey Noel-Voisin6, Anne Riu7, Gladys Ouedraogo7
1 L’Oréal Research and Innovation, , Aulnay-sous-Bois, France
2 Cosmetics Europe, Brussels, Belgium
3 Beiersdorf AG Hamburg, Germany
4 Procter & Gamble Technical Centres LTD, Reading, UK
5 Duncan Armstrong, Armstrong Pharmacology Ltd., Macclesfield, UK
6 L’Oréal Research and Innovation, Clichy, France
7 L’Oréal Research and Innovation, Aulnay-sous-Bois, France
P Presenting author

P-2a-8

Test Guideline No. 248 (XETA): a new OCDE approved alternative method for the identification of thyroid active chemicals

David Du PasquierP1, Gregory Lemkine1
1 Laboratoire Watchfrog
P Presenting author

P-2a-9

Relevant impact on the endocrine activity potential by modification of classical in vitro transactivation assays – exemplary case studies with two UV filters

Sylvie  EmeryP1, Guillaume Lereaux1, Sébastien  Grégoire1, Matthew Burbank1, Nicola Jane Hewitt2, Audrey Noel-Voisin3, Dagmar Bury3, Gladys Ouedraogo1, Anne Riu1
1 L’Oréal, Research and Innovation, Aulnay-sous-Bois, France
2 Cosmetics Europe, Brussels, Belgium
3 L’Oréal Research and Innovation, Clichy, France
P Presenting author

P-2a-10

INCORPORATING A METABOLIZING SYSTEM AND TOPICAL APPLICATION TO IMPROVE THE VALUE OF IN VITRO ENDOCRINE DISRUPTION ASSAYS

Sylvie EMERYP1, Guilaume  LEREAUX1, Matthew Burbank1, Sébastien GREGOIRE1, Dagmar  BURY2, Véronique  POULSEN2, Anne RIU3
1 L’Oréal, Research & Innovation, Aulnay-sous-Bois, France
2 L’Oréal Research and Innovation, Clichy, France.
3 L’Oréal Research and Innovation, Aulnay-sous-Bois, France
P Presenting author

P-2a-11

The agonistic bioanalytical equivalent concentration: A novel tool for assessing the endocrine activity of environmental mixtures

Martin EzechiášP1
1 Institute of Microbiology of the Czech Academy of Sciences
P Presenting author

P-2a-12

ENDOCRINE DISRUPTION – BREAKING FREE FROM IN VIVO TESTING ORTHODOXY

Emma GrangeP1
1 Cruelty Free Europe
P Presenting author

P-2a-13

PERFORMANCE LIMITATION OF OECD TEST NO. 455 ESTROGEN RECEPTOR TRANSACTIVATION ASSAY CLASSIFICATION CRITERIA USING VM7LUC4E2 CELL LINE.

Theo Le GodecP1, Poornima Paramasivan1, Michael McMahon2, Matilda  Bingham1
1 Concept Life Sciences
2 Penman Consulting
P Presenting author

P-2a-14

A metabolomics approach to investigate in vitro the hepatotoxicity of drugs and the mechanisms so far involved

Teresa Martinez-Sena1,2, Erika Moro1, Marta MorenoP1, Jose V. Castell1,3
1 Experimental Hepatology, IISLAFE
2 Analytical Chemistry, University of Valencia
3 Departament of Biochemistry and Molecular Biology
P Presenting author

P-2a-15

Validation of Endocrine Disrupter Assays at EURL ECVAM

Anne  MilcampsP1, Elise  Grignard1, Ingrid Langezaal1, Roman Liska1, Camilla Bernasconi1, Gerard Bowe1, Tom Cole1, Sandra Coecke1, Francesca Pistollato1, Valerie Zuang1, Sharon Munn1
1 European Commission
P Presenting author

P-2a-16

THE ADIPOGENESIS MODEL OF HUMAN MESENCHYMAL STEM CELLS FOR THE DETERMINATION OF OBESOGENIC POTENTIALS OF SUNSCREENS

Minsoo NohP1, Sungjin Ahn1, Seungchan An1, In Guk Park1, Seok Young Hwang1, Junpyo Gong1
1 College of Pharmacy, Seoul National University
P Presenting author

P-2a-17

INCREASING THE NUMBER OF AVAILABLE METHODS FOR THE DETECTION OF ENDOCRINE DISRUPTORS BEYOND EATS

Andrea RIVERO-ARZEP1, Elise GRIGNARD1, Philippe HUBERT1
1 PEPPER – Paris (FR)
P Presenting author

P-2a-18

EVALUATION OF THE CYTOTOXICITY OF TETRAHYDROCANNABINOL,CANNABINOL AND CANNABIDIOL IN PRECURSOR CELLS OF THE GLIA

Rene  RochaP1, Javier  Figueroa1, Diego Diaz1, Boris Duffau1, Ivan  Triviño1
1 Instituto de Salud Publica de Chile
P Presenting author

P-2a-19

Grouping of chemicals into mode of action classes by automated effect pattern analysis using the zebrafish embryo toxicity test

Elisabet TeixidoP1, Odile Kerkhof2,3, Nils Klüver2, Tobias R Kiessling4, Stefan Scholz2
1 GRET-Toxicology Unit, Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028, Barcelona, Spain.
2 Department of Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research-UFZ, Permoserstraβe 15, 04318, Leipzig, Germany.
3 French agency for food, environmental and occupational health and safety, Anses, France.
4 Scientific Software Solutions, 04275, Leipzig, Germany.
P Presenting author

P-2a-20

Study of Endocrine disruptors-related lipid and carbohydrate metabolism disorders in human hepatocytes

Charbel ToumaP1, Kevin BERNAL2, Frederic  EZAN 1, Sophie  ROSE 1, Antoine LEGRAND1, Valentine BROURAD1, Béatrice LE-GRAND2, Corinne CHOULY1, Dominique LAGADIC1, Etienne BLANC2, Sophie LANGOUËT1
1 Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail) – UMR_S 1085, 35000 Rennes, France
2 Inserm UMR-S 1124, T3S, Université Paris Cité, 75006 Paris, France
P Presenting author

P-2a-21

OXYSTEROLS PROFILE IN ZEBRAFISH EMBRYOS UPON EXPOSURE TO BISPHENOL A (BPA) AT 8 AND 24 HOURS POST-FERTILISATION

Anton VremereP1, Carmine Merola2, Federico Fanti2, Manuel Sergi2, Monia Perugini2, Dario Compagnone2, Michele Amorena2, Milena Mikhail1, Stefano Lorenzetti1
1 ISS – Istituto Superiore di Sanità, Department of Food Safety, Nutritionn and Veterinary Public Health
2 University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment
P Presenting author

P-2a-22

HIGH CONTENT SCREENING OF BISPHENOLS AND THEIR MIXTURES UNDER CONDITIONS OF LOW-INTENSITY ADIPOGENESIS OF HUMAN MESENCHYMAL STEM CELLS (hMSCs)

Kalle Norgren1, Astrud Tuck1, Antero Vieira Silva1, Paula Burkhardt1, Mattias Öberg1, Vesna Munic KosP1
1 Karolinska Institutet
P Presenting author

2b. Challenges in cosmetics safety 

P-2b-1

An OECD 439 Adaptive In Vitro Skin Irritation Test Model

Sevde  AltuntasP1,2,3, Ahmet Katı1,4,3, Ashwani  Sharma5
1 Experimental Medicine Research and Application Center, University of Health Sciences Turkey, Uskudar, Istanbul, Turkey
2 Department of Tissue Engineering, Institution of Health Sciences, University of Health Sciences Turkey, Uskudar, Istanbul, Turkey
3 Omic-A Research and Innovation Ltd Company, Pendik, 34668, Istanbul, Turkey
4 Department of Tissue Biotechnology, Institution of Health Sciences, University of Health Sciences Turkey, Uskudar, Istanbul, Turkey
5 Insight Biosolutions, 35000, Rennes, France
P Presenting author

P-2b-2

ToxTool: an innovative database for the cosmetic regulatory affair

Giulia De Negri AtanasioP1, Federica Robino2, Lorenzo Dondero1, Elisabetta Perata2, Francesca  Rispo1, Giorgia Allaria1, Francesca  Tardanico1, Francesca Bruzzone2, Matteo Zanotti-Russo2, Elena Grasselli1
1 Department of Earth, Environment and Life Science, University of Genoa, Genova, Italy
2 ANGEL CONSULTING S.a.s., Italy
P Presenting author

P-2b-3

Too many Animal Tests on Cosmetic Ingredients for REACH in the EU: actual situation and future prospects

Costanza RovidaP1, Francois Busquet2
1 CAAT-Europe, Konstanz University, Germany
2 Altertox, Belgium
P Presenting author

P-2b-4

The study on EDs and the application of the NGRA according to the guidelines SCCS NoG XI Ed: two overlapping challenges.

Matteo Zanotti RussoP1, Giorgia Allaria2, Lorenzo Dondero2, Giulia De Negri Atanasio2, Francesca Rispo2, Francesca Tardanico2, Federica Robino1, Elisabetta Perata1, Francesca Bruzzone1, Elena Grasselli2
1 Angel Consulting S.a.s. 20122 Milano Italy
2 Dipartimento Scienze della Terra dell’Ambiente e della Vita-DISTAV, Università degli Studi di Genova—16132 Genova, Italy
P Presenting author

3a. Models, biomarkers and assays for systemic and immune toxicity  

P-3a-1

Analysis of the predictive capacity of the SENS-IS assay to define the skin sensitization potency on 186 chemicals

Françoise Cottrez1, Elodie Boite1
1 ImmunoSearch, Grasse, France
P Presenting author

P-3a-2

Potential beneficial effects of selenium nanoparticles in the adjuvant anticancer therapy

Elena Ines Adam-DimaP1, Mihaela Balaș2, Anca Dinischiotu2, Carmen Nicoleta Purdel1, Denisa  Margină1
1 “Carol Davila” University of Medicine and Pharmacy Bucharest, Faculty of Pharmacy
2 University of Bucharest, Faculty of Biology
P Presenting author

P-3a-3

Extending the boundaries of OECD DPRA test N°442C towards complex substances and mixtures: an innovative feat in HPLC-MS/MS and skin sensitization testing

Eric  Andres P1, Sergio Gonzalez Duque1, Axelle Huyard1, Rola Barcham1, Christophe Dini1
1 Oroxcell
P Presenting author

P-3a-4

Target organs in cosmetics: which in vitro and in silico models to move towards to increase expertise in systemic toxicity?

Matthew BurbankP1, Emilie Barbeau1, Romain Grall1, Ann Detroyer1, Audrey Noel-Voisin2, Gladys Ouedraogo1, Anne Riu3
1 L’Oréal Research and Innovation, , Aulnay-sous-Bois, France
2 L’Oréal Research and Innovation, , Clichy, France
3 L’Oréal Research and Innovation,, Aulnay-sous-Bois, France
P Presenting author

P-3a-5

Safety of cosmetic perfumes screened in chemico and in vitro in combination with targeted chemical analysis

Marketa  DvorakovaP1,2, Kristina Kejlova1, Hana Bendova1, Lada Svobodova1,3, Marian Rucki1, Vaclav Sevcik1,4, Barbora Hosikova3, Jan Chrz3, Danuse Ocadlikova1, Hana Kolarova3, Marek Maly1, Dagmar Jirova1, Helena Kandarova5
1 National Institute of Public Health, Prague, Czech Republic
2 Third Faculty of Medicine, Charles University in Prague, Czech Republic
3 Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
4 Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Czech Republic
5 Slovak Academy of Sciences, Bratislava, Slovak Republic
P Presenting author

P-3a-6

Use of Assay Ready KertainoSens® Cells to test for Skin Sensitization

Lukas FockeP1, Valerie de Boor1, Oliver Wehmeier1
1 acCELLerate GmbH
P Presenting author

P-3a-7

A predictive 3D liver spheroid model for the assessment of liver repeated dose toxicity

Romain GrallP1, Veronique Neiveyans1, Sylvie Emery1, Françoise Gautier2, Gladys Ouedraogo1, Anne Riu1
1 L’Oréal Research and Innovation, Aulnay-sous-Bois, France
2 L’Oréal Research and Innovation, Clichy, France.
P Presenting author

P-3a-8

Developing an innervated skin model for predicting neuroinflammatory and neurosensory effect of cosmetic compounds

Md Zobaer HasanP1, Amy Leigh Harding2, Hirofumi Nakanishi1, Tetsuo Furuno1, Craig  Murdoch2, Helen E Colley2
1 Rohto Pharmaceutical CO.,ltd
2 University of Sheffield
P Presenting author

P-3a-9

EFFECTS OF PER- AND POLYFLUOROALKYL SUBSTANCES IN HUMAN B AND T CELL LINES

Aafke JanssenP1, Karsten Beekmann1, Nicole Pinckaers1, Joke  Hoekstra1, Sanne  Schild1, Ron Hoogenboom1, Ad Peijnenburg1, Jochem Louisse1
1 Wageningen Food Safety Research
P Presenting author

P-3a-10

IN VITRO 3D KIDNEY MODEL CO-CULTURED WITH HUMAN IMMUNE CELLS INVESTIGATING IMMUNE HYPERACTIVITY ASSOCIATED RENAL INJURY

Yu Bin LeeP1, Mi-lang Kyun1, Tamina Park1, Min-Heui Yoo1, Inhye Kim1, Hyewon Jung1, Myeongjin Choi1, Seo Yule Jeong1, Jihye Son1, Dong Ho Woo1, Chang Hoon Choi1, Hyun-A Oh1, Ye-Ji Kim1, Gong Yeon Kim1, Yu Yeong Jeong1, Daeui Park1, Kyoung-Sik Moon1
1 Korea Institute of Toxicology
P Presenting author

P-3a-11

Establishment of a complex commensal 3-D skin model for toxicity testing

Lisa LemoineP1, Maya Kissner, Aline Rosin, Tewes Tralau
1 German Federal Institute for Risk Assessment (BfR)
P Presenting author

P-3a-12

EXPLORING CAUSAL RELATIONSHIPS BETWEEN PFAS EXPOSURE AND LIPID HOMEOSTASIS BY THE USE OF A HUMAN LIVER SPHEROID MODEL

Birgitte LindemanP1, Marit Låg1, Line Småstuen Haug1, Azemira Sabaredzovic 1, Kristine Bjerve Gützkow1
1 Norwegian Institute of Public Health; Division of Climate and Environmental Health
P Presenting author

P-3a-13

In vitro study of biosafety of ZnO nanoparticles: coagulation assay and protein corona.

Montserrat MitjansP1, Marc Bilbao1, Laura Marics1, M. Pilar Vinardell1
1 Fisiologia, Dep. de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona
P Presenting author

P-3a-14

Evaluation of the use of in vitro methods to study the general toxic effect of cosmetic products

Ulyana ProtasevichP1, Maryna Anisovich1, Tatsiana  Hamolka1, Iryna Ilyukova1
1 Republican unitary enterprise «Scientific practical centre of hygiene»
P Presenting author

P-3a-15

LIVER METABOLOMICS IN VITRO– A MINIATURIZED SCREENING APPROACH TO PREDICT THE MODE OF ACTION OF LIVER TOXICANTS IN HEPG2 CELLS

Sabina Ramirez HincapieP1, Barbara  Birk1, Michael  Herold2, Philipp  Ternes2, Volker  Haake2, Varun  Giri1, Franziska Maria  Zickgraf 1, Hans-Albrecht  Huener1, Andreas  Verlohner1, Hennicke  Kamp1, Robert  Landsiedel1, Elke  Richling3, Dorothee  Funk-Weyer1, Bennard  van Ravenzwaay1
1 BASF SE
2 BASF Metabolome Solution GmbH
3 University of Kaiserslautern
P Presenting author

P-3a-16

IMPLEMENTING AN IN VITRO METABOLOMICS SCREENING METHOD TO STUDY LIVER TOXICANTS IN HEPG2 CELLS- A CASE STUDY WITH NITROFURANTOIN

Sabina Ramirez HincapieP1, Barbara  Birk1, Franziska Maria Zickgraf1, Michael  Herold2, Philipp Ternes2, Volker  Haake2, Varun  Giri1, Hans-Albrecht  Huener1, Andreas  Verlohner1, Hennicke  Kamp1, Robert  Landsiedel1, Elke  Richling3, Dorothee  Funk-Weyer1, Bennard  van Ravenzwaay1
1 BASF SE
2 BASF Metabolome Solutions GmbH
3 University of Kaiserslautern
P Presenting author

P-3a-17

IMPACT OF BENZO[A]PYRENE ON MICRORNAS PROFILES IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS AND THEIR DISCHARGED EXTRACELLULAR VESICLES

Rima SoukiP1, Jeremy  Amosse1, Maguy  Basbous2, Eric  Le Ferrec1, Dominique Lagadic-Gossmann1, Christine Demeilliers2, Anne Maitre2, Lydie Sparfel1
1 Univ Rennes, Inserm, EHESP, Irset (Institut de recherche en santé, environnement et travail),  UMR_S 1085, F-35000 Rennes, France
2 Univ. Grenoble Alpes, CNRS, Grenoble INP, CHU Grenoble Alpes, TIMC Laboratory (UMR CNRS 5525), EPSP Team, 38000, Grenoble, France
P Presenting author

P-3a-18

Update and optimization of an adverse outcome pathway network of chemical-induced cholestasis

Jonas van ErtveldeP1, Anouk Verhoeven1, Tamara Vanhaecke1, Ramiro Jover2, Jian  Jiang1, Mathieu  Vinken1
1 Entity of In Vitro Toxicology and Dermato Cosmetology, Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
2 Joint Research Unit in Experimental Hepatology, University of Valencia; IIS Hosp. La Fe; CIBERehd; Spain
P Presenting author

P-3a-19

An adverse outcome pathway network for liver steatosis induced by chemicals

Anouk VerhoevenP1, Jonas  van Ertvelde 1, Mathieu Vinken1, Robim Marcelino Rodrigues1, Joost Boeckmans1, Alexandra  Gatzios1, Sara  Sepehri1, Marth  Stinckens1, Ramiro  Jover2, Anna  Rapisarda2, Birgitte  Lindeman3, Graciela Lopez Soop3, Camilla  Svendsen3, Jian  Jiang1, Tamara  Vanhaecke1
1 Entity of In Vitro Toxicology and Dermato-Cosmetology, Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
2 Joint Research Unit in Experimental Hepatology, University of Valencia; IIS Hosp. La Fe; CIBERehd; Spain
3 Department of Chemical Toxicology, Norwegian Institute of Public Health, Oslo, Norway
P Presenting author

3b. Organ-on-a-chip & Microphysiological Systems   

P-3b-1

On the Real-Time Oxygen Consumption of Hepatocytes in a Microphysiological System

Marie Flechner1, Jan Martin Gebler1, Christian Gehre1, Sarah Kammerer2, Dennis Kaden3, Jan-Heiner Küpper2, Claus Duschl1, Lars Daehne3, Katja UhligP1
1 Fraunhofer Institute for Cell Therapy and Immunology (IZI-BB), 14476 Potsdam, Germany
2 Brandenburg University of Technology, 01968 Senftenberg, Germany
3 Surflay Nanotec GmbH, 12489 Berlin, Germany
P Presenting author

P-3b-2

PROPOSAL FOR A HUMAN FOETO-PLACENTAL ORGAN-A-CHIP FOR TESTING DEVELOPMENTAL TOXICITY

Emilio Benfenati1, Michaela Luconi2, Udo R. Markert3, Tobias May4, Alessandra Roncaglioni1, Astrid Schmidt5, Miguel A. SogorbP6, Marco Straccia7, Sabrina Tait8, Susanne Wolbankh9
1 Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milano, Italy
2 Dept. Clinical & Experimental Biomedical Sciences, University of Florence, Italy
3 Placenta Lab, Department of Obstetrics, University Hospital Jena, Am Klinikum 1, 07747, Jena, Germany.
4 InSCREENeX GmbH, Braunschweig, Germany
5 Placenta Lab, Department of Obstetrics, University Hospital Jena, Am Klinikum 1, 07747, Jena, Germany
6 Bioengineering Institute, Miguel Hernández de Elche University, 03202-Elche, Spain
7 FRESCI by SCIENCE&STRATEGY SL, Barcelona, Spain
8 Center for Gender-Specific Medicine, Istituto Superiore di Sanità, Rome, Italy
9 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Donaueschingenstrasse 13, A-1200, Vienna, Austria
P Presenting author

P-3b-3

ALL-IN-ONE MICROFLUIDIC-BASED ROBOTIC PLATFORM FOR AUTOMATED TOX SCREENINGS IN C. ELEGANS.

Elena  Katsyuba1, Lazar Stojkovic1, Fabien Tâche1, Matteo Cornaglia1, Laurent MouchiroudP1
1 Nagi Bioscience SA, EPFL Innovation Park, Chemin de la Dent d’Oche 1B, CH-1024 Ecublens, Switzerland
P Presenting author

P-3b-4

Mutagenicity and genotoxicity assessment of a new biopreservative product rich in Enterocin AS-48

Antonio Cascajosa-LiraP1, Ana Isabel Prieto1, María Puerto1, Enrique  Guillamon2, Alberto Baños2,3, Eva  Valdivia3, Ángeles  Jos1, Ana María Cameán1
1 Area of Toxicology. Faculty of Pharmacy.Universidad de Sevilla, Profesor García González n°2, 41012 Sevilla, España
2 DMC Research Center. Camino de Jayena, 82, 18620 Alhendín, Granada, España
3 Department of Microbiology. Universidad de Granada. Campus de Fuente Nueva.Dr. Severo Ochoa, 18071 Granada, España
P Presenting author

P-3b-5

A multiorgan-on chip platform for the in vitro investigation of off-target cardiotoxicity of liver-metabolized anticancer drugs

Erika FerrariP1, Roberta Visone1,2, Paola Occhetta1,2, Marco  Rasponi1,2
1 Department of Electronics, Information and Bioengineering (DEIB), Politecnico di Milano, Milano, Italy
2 BiomimX Srl, Milano, Italy
P Presenting author

P-3b-6

3D human vasculature-on-a-chip: the biological effect of combustible cigarette smoke and vapor from three heated tobacco products on monocyte adhesion to vessels comprising coronary artery endothelial cells

Ayaka HayashidaP1, Atsuko  Nozawa1, Kazuhiro  Ohashi1, Shigeaki Ito1
1 Scientific Product Assessment Center, Japan Tobacco Inc.
P Presenting author

P-3b-7

What are the barriers to adoption of new approach methodologies for gastrointestinal toxicity testing? A systematic review of in vitro models of  gastrointestinal toxicity

Alasdair  IrvineP1, Paul Jochems1, Roos Masereeuw1
1 Utrecht University
P Presenting author

P-3b-8

A systematic review of in vitro models of drug-induced liver injury

Alasdair  IrvineP1, Vivian  Lehman2,3, Bart  Spee4, Sabine  Fuchs2, Ross Masereeuw 5, Mathieu  Vinken6
1 Utrecht Institute for Pharmaceutical Sciences (UIPS), Division of Pharmacology, Utrecht University
2 Department of Metabolic Diseases, University Medical Center Utrecht
3 Department of Veterinary Medicine, Utrecht University
4 Department of Veterinary Medicine, Utrecht University,
5 Utrecht Institute for Pharmaceutical Sciences (UIPS), Division of Pharmacology, Utrecht University
6 Department of In Vitro Toxicology, Vrije Universiteit Brussel
P Presenting author

P-3b-9

USE OF MICROFLUIDIC CHAMBERS IN ENVIRONMENTAL TOXICITY STUDIES USING FISH CELLS AS A MODEL

Gemma Lopez P1, Cinta  Porte1, Xavier  Muñoz2, Carlos  Barata1
1 IDAEA-CSIC
2 IMB-CNM-CSIC
P Presenting author

P-3b-10

SOLUBLE FACTORS ARE INVOLVED IN THE PROPAGATION OF LIVER FIBROSIS IN VITRO

Saskia SchmidtP1,2, Catherine Messner1,3, Carine Gaiser1, Bradley Petkus4, Loïc  Burr4, Joy Roth5, Alexandra Homsy5, Felix Kurth4, Silvia Generelli4, Laura Suter-Dick1,3
1 School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, 4132, Switzerland
2 Department of Pharmaceutical Sciences, University of Basel, Basel, 4056, Switzerland
3 SCAHT: Swiss Centre of Applied Human Toxicology, Basel, 4055, Switzerland
4 Centre Suisse d’Electronique et de Microtechnique SA (CSEM), Landquart, 7302, and Muttenz, 4132, Switzerland
5 Haute Ecole Arc ingénierie (HES-SO), La Chaux-de-Fonds, 2300, Switzerland
P Presenting author

4a. Computational toxicology – in silico modelling, read-across, artificial intelligence and machine learning   

P-4a-1

Adverse Outcome Network For Obesity Initiated By Endocrine Disruptors developed using the AOP-helpFinder tool

Kevin Bernal1, Florence Jornod1, Xavier Coumoul1, Etienne Blanc1, MinJi Kim2, Karine AudouzeP1
1 Université Paris Cité, Inserm U1124, 75006 Paris, France
2 Université Sorbonne Paris Nord, Inserm U1124, 75006 Paris, France
P Presenting author

P-4a-2

Development of a easy-to-use, semantic technology-based knowledgebase containing toxicological information of cosmetic ingredients to assist animal-free risk assessment

Sara SepehriP1 , Robim M. Rodrigues1 , Mona Delagrange1 , Joery De Kock1 , Audrey Sanctorum 2 , Jan Maushagen 2 , Christophe Debruyne 3, Olga De Troyer 2 , Tamara Vanhaecke1
1 Department of In Vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
2 WISE lab, Vrije Universiteit Brussel, Brussels, Belgium
3 Montefiore Institute of Liège University, Quartier Polytech 1, Allée de la Découverte 10, 4000 Liège, Belgium

P Presenting author

P-4a-3

Three steps to select analogues for skin sensitization prediction using read-across: an exemplary case study with vanillin

Françoise Gauthier1, Fleur Tourneix1, Hind Assaf-Vandecasteele1, Dagmar Bury1, Nathalie AlépéeP1
1 L’Oreal R&I
P Presenting author

P-4a-4

Elucidating the Inhibition Mechanism of six FDA-Approved Drugs on P-glycoprotein (P-gp) Transporter by Molecular Docking Simulation

Abira Dey1, Ruoya Li2, Nathalie Larzat2, Jean Bernard  Idoipe2, Ahmet Kati3, Ashwani SHARMAP2
1 Indian Science and Technology Foundation, Delhi-110053, India
2 Insight Biosolutions, 35000, Rennes, France
3 Experimental Medicine Research and Application Center, University of Health Sciences Turkey, Uskudar, Istanbul, Turkey, Department of Biotechnology, Institution of Health Sciences, University of Health Sciences Turkey, Uskudar, Istanbul, Turkey,
P Presenting author

P-4a-5

ORBITOX – A COMPUTATIONAL TRANSLATIONAL DISCOVERY PLATFORM FOR DATA MINING AND READ-ACROSS

Alexander  Sedykh1, Austin  Ross1, Vijay  Gombar1, Nicole  Kleinstreuer2, Alex  Merick3, Ruchir  Shah1, Warren  Casey3
1 Sciome LLC
2 NICEATM, NIEHS
3 NTP, NIEHS
P Presenting author

P-4a-6

Use of text-mining and chemoinformatics as additional decision-making tools for the chemical selection process of the EU-NETVAL thyroid validation study

David  AsturiolP1, Alessandro  Antonelli2, Anna  Beronius3, Patience Browne4, Thomas  Cole1, Raffaella Corvi1, Barbara  Demeneix5, Alexius  Freyberger6, Mary Gilbert Gilbert7, Elise  Grignard1, Klára Hilscherová8, Aude Kienzler1, Dries Knapen9, Josef  Kӧhrle10, Ingrid Langezaal1, Sharon  Munn1, Amalia  Munoz Pineiro1, Gea  Oliveri Conti11, Alicia  Paini1, Alex  Patak1, Mauro Petrillo1, Daniel  Pickford12, Francesca  Pistollato1, Anna  Price1, Antonio  Puertas Gallardo1, Maddalena  Querci1, Barbara Raffael1, Kostja  Renko10, Tammy  Stoker7, Jukka  Sund1, Guy  Van den Eede13, Michael  Wade14, Maurice Whelan1, Sandra Coecke1
1 European Commission, Joint Research Centre, Italy
2 University of Pisa, Italy
3 Karolinska Institutet, Sweden 
4 OECD, France
5 National Museum of  Natural History
6 Bayer AG
7 US Environmental Protection Agency, USA
8 Masaryk University, Czechia
9 University of Antwerp, Belgium
10 Charité-Universitӓtsmedizin, Germany
11 University of Catania, Italy
12 Syngenta, UK
13 European Commission, Joint Research Centre
14 Health Canada, Canada
P Presenting author

P-4a-7

IMPLEMENTATION OF IN SILICO-BASED READ-ACROSS ASSESSMENT FOR GENOTOXICITY FOR PESTICIDE RESIDUES UNDER EU LEGISLATION AND KEY CHALLENGES

Minako  AllenP1, Maria  Pellizzaro 1, Rebecca Silcock1
1 Exponent International Ltd
P Presenting author

P-4a-8

Development and validation of machine learning based QSAR models to predict the TPO inhibitors

Bharath BRP1, Vaibhav Barot1, Rahul Date1, Abhay Deshpande1
1 Jai Research Foundation
P Presenting author

P-4a-9

High throughput in vitro genotoxicity methods for hazard identification and characterization of “data poor” compounds

Steve BryceP1, Jeff Bemis1, Stephen  Dertinger1, Stephanie Smith-Roe2, Kristine Witt2
1 Litron Laboratories, Rochester NY
2 Division of the National Toxicology Program, NIEHS, Research Triangle Park, NC
P Presenting author

P-4a-10

Combining gene expressions and imaged-based morphological features for chemical-phenotype profiles

Natacha CerisierP1, Bryan Dafniet1, Olivier Taboureau1
1 Université Paris Cité, INSERM U1133, CNRS UMR8251, 75006, Paris, France.
P Presenting author

P-4a-11

Development and Translation of Quantitative Neuronal Adverse Outcome Pathway (qNAOP) model for Neurotoxicity Risk Assessment

Deepika DeepikaP1, Raju Prasad Sharma1, Marta Schuhmacher1, Vikas Kumar1
1 Environmental Engineering Laboratory, Departament d’Enginyeria Quimica, Universitat Rovira i Virgili, Av. Països Catalans 26, 43007 Tarragona, Catalonia, Spain
P Presenting author

P-4a-12

CONTRIBUTION MAPPING: USING STRUCTURE–TOXICITY RELATIONSHIPS (STR) AND MECHANISTIC INTERPRETABILITY OF IN SILICO MODELS TO ASSESS DEVELOPMENTAL TOXICITY AND ENDOCRINE-DISRUPTING POTENTIAL OF TWELVE UV FILTERS

Andrezza Di Pietro Micali CanavezP1, Carlos  Eduardo Matos dos Santos 2, Desiree Cigaran Schuck1, Marcio  Lorencini1
1 Grupo Boticário
2 Altox Ltd
P Presenting author

P-4a-13

Physiological map to study kidney toxicity in the ONTOX project

Alessio GambaP1, Luiz Carlos Maia Ladeira1, Raphaëlle Lesage2, Daniël Roodzant3, Devon Barnes4, Manoe Janssen4, Marc Teunis3, Rosalinde Masereeuw4, Liesbet Geris1,2,5, Bernard Staumont1
1 Biomechanics Research Unit, GIGA In Silico Medicine, University of Liège, Belgium.
2 Skeletal Biology and Engineering Research Center, KU Leuven, Belgium.
3 Innovative testing in Life Sciences & Chemistry, Utrecht University of Applied Sciences, The Netherlands.
4 Div. Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands.
5 Biomechanics Section, Department of Mechanical Engineering, KU Leuven, Belgium.
P Presenting author

P-4a-14

QUANTITATIVE ADVERSE OUTCOME PATHWAY MODELING FOR CHRONIC TOXICITY

Shigeaki ItoP1, Sayak Mukherjee2, Kazuo Erami1, Shugo Muratani1, Akina Mori1, Sakuya Ichikawa1, Kei Yoshino1, Dawn Fallacara2
1 Scientific Product Assessment Center, Japan Tobacco Inc., 6-2, Aoba-ku, Yokohama, Japan
2 Battelle, 505 King Ave., Columbus, OH 43201
P Presenting author

P-4a-15

Applying machine-learning approaches to identify key genes associated with drug-induced cholestasis

Jian jiangP1, Jonas van Ertvelde1, Gökhan Ertaylan2, Ralf Peeters3,4, Danyel Jennen5, Theo de Kok3,5, Mathieu Vinken1
1 Entity of In Vitro Toxicology and Dermato‑Cosmetology, Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
2 Vlaamse Instelling voor Technologisch Onderzoek (VITO) NV, Health, Boeretang 200, 2400 Mol, Belgium
3 Maastricht Centre for Systems Biology (MaCSBio), Maastricht University, Maastricht, The Netherlands
4 Department of Data Science and Knowledge Engineering, Maastricht University, Maastricht, The Netherlands
5 Department of Toxicogenomics, GROW School for Oncology and Reproduction, Maastricht University, Maastricht, The Netherlands
P Presenting author

P-4a-16

Virtual Human Platform for Safety Assessment (VHP4Safety): Assessing the safety of chemicals and pharmaceuticals based on human data

Anne KienhuisP1, Cyrille Krul2, Juliette Legler3
1 National Institute for Public Health and the Environment (RIVM)
2 University of Applied Sciences Utrecht (HU)
3 Utrecht University
P Presenting author

P-4a-17

A COMPARATIVE COMPUTATIONAL PREDICTION OF THE BINDING OF ANDROGEN- AND ESTROGEN-LIKE FLAVONOIDS TO THEIR COGNATE (NON)NUCLEAR RECEPTORS

Stefano LorenzettiP1, Giulia D’Arrigo2, Eleonora Gianquinto2, Giulia Rossetti3, Gabriele Cruciani4, Francesca Spyrakis2
1 ISS – Istituto Superiore di Sanità, Department of Food Safety, Nutritionn and Veterinary Public Health
2 University of Turin, Department of Drug Science and Technology
3 Aachen University, Department of Neurology
4 University of Perugia, Department of Chemistry, Biology and Biotechnology
P Presenting author

P-4a-18

Designing physiological maps as a tool to study liver toxicology

Luiz Carlos Maia LadeiraP1, Alessio Gamba1, Raphaëlle Lesage2, Anouk Verhoeven3, Jonas  van Ertvelde3, Jian Jiang3, Daniël Roodzant4, Marc Teunis4, Ramiro Jover5, Tamara Vanhaecke3, Mathieu Vinken3, Harm  Heusinkveld6, Liesbet Geris1,2,7, Bernard Staumont1
1 Biomechanics Research Unit, GIGA In Silico Medicine, University of Liège, Belgium
2 Skeletal Biology and Engineering Research Center, KU Leuven, Belgium
3 Department of In Vitro Toxicology and Dermato-cosmetology, Vrije Universiteit Brussel, Belgium
4 Innovative Testing in Life Sciences & Chemistry, University of Applied Sciences Utrecht, The Netherlands
5 Dept. Biochemistry, University of Valencia, IIS Hosp. La Fe, CIBERehd, Spain
6 Centre for Health Protection, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands
7 Biomechanics Section, Department of Mechanical Engineering, KU Leuven, Belgium
P Presenting author

P-4a-19

Toxicity prediction of mycotoxins by in silico modeling

Martina Palomino-SchätzleinP1, Josefa Tolosa2, Eva Serrano-Candelas1, Jose Luis Vallés-Pardo1, Salvador  Moncho1, Rafael Gozalbes1
1 ProtoQSAR SL, CEEI, Parque Tecnológico de Valencia (Av. Benjamin Franklin 12, 46980, Paterna, Valencia, Spain)
2 Laboratory of Toxicology. Faculty of Pharmacy. University of Valencia (Av. Vicent Andrés Estellés s/n. 46100. Burjasot, Valencia, Spain)
P Presenting author

P-4a-20

Evaluation of state-of-the-art in silico testing methods to fill physico-chemical and toxicokinetic data gaps within the ONTOX project

Alessandra RoncaglioniP1, Domenico Gadaleta1, Erika Colombo1, Eva Serrano-Candelas2, Pablo Aparicio2, Rafael Gozalbes2, Emilio Benfenati1
1 Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy
2 ProtoQSAR SL, Valencia, Spain
P Presenting author

P-4a-21

Development new web-tool for phototoxicity prediction on the base of machine learning approach

Marta Šoltésová PrnováP1,2, Matej Halinkovič2, Tibor Sloboda2, Kateřina Mušková2, Patrik Pavlačka2, Jakub Knánik2, Helena Kanďárová1
1 Institute of Experimental Pharmacology and Toxicology, CEM, Slovak Academy of Sciences, Bratislava, Slovakia
2 Faculty of Informatics and Information Technologies Slovak University of Technologies in Bratislava, Slovakia
P Presenting author

P-4a-22

QSAR MODEL FOR PREDICTING MYCOTOXIN MUTAGENICITY

Josefa TolosaP1, Martina Palomino-Schätzlein2, Eva Serrano-Candelas2, José Luis Vallés-Pardo2, Salvador Moncho2, Rafael Gozalbes2
1 University of Valencia
2 ProtoQSAR
P Presenting author

4b. Local toxicity testing (safety and efficacy)

P-4b-1

Concentration, typology of surfactants, in vitro and clinical ocular tolerance studies: a multi-parameterized approach for foaming cosmetics intended to claim “do not sting the eyes”

Rakotomalala Sandrine1, Berrada-Gomez Marie-PierreP1, Baco David2, Ferret Pierre-Jacques1
1 Safety Assessment Department, Pierre Fabre Dermo-Cosmétique, Toulouse, France
2 Global Innovation Center Department, Pierre Fabre Dermo-Cosmétique, Toulouse, France
P Presenting author

P-4b-2

Effect of a modulator on the skin sensitization potency of cosmetic fragrance formulations

Carine LINOSSIER1, Marine CUSCUSA1, Marisa MELONI2, Andy FORRERYD3, Mylène LANVIN1
1 COTY
2 VITROSCREEN
3 SENZAGEN
P Presenting author

P-4b-3

Comparison between HET-CAM protocols and a product use clinical study for eye irritation evaluation of personal care products according to their surfactant composition

Martín Nicolás Rivero1, Mariela Lenze1, Mercedes Izaguirre1,2, Martina Benedetti1,2, Silvia Pérez Damonte3, Silvia Wikinski1,2, María Laura GutiérrezP1,2
1 Institute of Pharmacology – University of Buenos Aires
2 CONICET
3 CLAIM CRO
P Presenting author

P-4b-4

DECISION TREE FOR OCULAR SAFETY ASSESSMENT OF AGROCHEMICAL FORMULATIONS

Martín Nicolás Rivero1, Mariela Lenze1,2, Agustina Reschini1, Juan Ignacio Pina3, Romina Martinez4, Silvia Wikinski1,2, María Laura GutiérrezP1,2
1 Institute of Pharmacology – University of Buenos Aires
2 CONICET
3 ATANOR SCA
4 Dr Julio Mendez Hospital
P Presenting author

P-4b-5

THE RECONSTRUCTED HUMAN EPIDERMIS (RHE) EFFICIENCY IN THE CLASSIFICATION OF BIOLOGICAL PRODUCT BY THE IN VITRO IRRITATION AND CORROSION TESTS (OECD 439 AND 431).

Bruna Assunção Bechtold1, Julio César Cianci2, Maria ´Paula Mancini Coelho3, Leandro Fernando Felix4, Thatiane Nunes Santana5, Vanja Dakic6, Rodrigo De Vecchi6, Luis Paulo Fava7, Priscilla Muniz Ribeiro da Silva8, Juliana Falcato VecinaP8
1 Specialist, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil
2 Laboratory Assistant, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil
3 Laboratory Analyst; Merieux NutriSciences (Bioagri Laboratórios Ltda)
4 Trainee, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil
5 Laboratory technique, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil
6 Researcher, Episkin Academy, Rio de Janeiro-RJ, Brazil
7 Coordinator; Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
8 Researcher; Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
P Presenting author

P-4b-6

PROFICIENCY DEMONSTRATION OF MERIEUX NUTRISCIENCE (BIOAGRI LABORATÓRIOS LTDA) ON IN VITRO EYE IRRITATION AND SERIOUS EYE DAMAGE IMPLEMENTATION

Maria Paula Mancini Coelho1, Bruna Assunção Bechtold2, Julio César Cianci3, Larissa Gabriele Costa3, Leandro Fernando Felix4, Thatiane Nunes Santana5, Vanja Dakic6, Rodrigo De Vecchi6, Luis Paulo Fava7, Priscilla Muniz Ribeiro da Silva8, Juliana Falcato VecinaP8
1 Laboratory Analist; Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
2 Specialist, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
3 Laboratory Assistant, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
4 Trainee, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
5 Laboratory Technique, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
6 Researcher, Episkin Academy, Rio de Janeiro-RJ, Brazil
7 Coordinator, Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
8 Researcher; Mérieux NutriScience / Bioagri Laboratórios Ltda, Piracicaba-SP, Brazil.
P Presenting author

P-4b-7

SkinEthic™ HCE Time-to-Toxicity : World’s first adopted new approach methodology on its own for eye hazard identification adopted by OECD

Nathalie Alépée1, Frédéric AmaralP1, Virginie Leblanc1, Laurent Nardelli1, Valérie Tagliati2, Séverine Teluob2, Anaelle Viricel2, Valérie Michaut1
1 L’Oreal R&I
2 Episkin SA
P Presenting author

P-4b-8

COMPOUND A-111 – A NOVEL SMALL MOLECULE CANDIDATE FOR THE TOPICAL TREATMENT OF HYPERPIGMENTATION

Agnieszka Gunia-Krzyżak1, Justyna Popiół2, Magda Borczuch-Kostańska1, Katarzyna Wójcik-Pszczoła2, Paulina Koczurkiewicz-Adamczyk2, Patryk Kasza3, Przemysław Szafrański3, Dorota  Żelaszczyk1, Elżbieta Pękala2
1 Department of Bioorganic Chemistry, Chair of Organic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland
2 Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland
3 Chair of Organic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland
P Presenting author

P-4b-9

Corneal-derived biomolecular solution: application as an in chemico method for ocular toxicity assessment

Jordana Andrade Santos1, Wanessa Amorim Dias1, Artur Christian  Garcia da SilvaP1, Marize  Campos Valadares1
1 Laboratory of Research and Education in In Vitro Toxicology, Federal University of Goiás, Goiânia, Goiás, Brazil
P Presenting author

P-4b-10

A new approach for Eye Hazard Assessment of surfactants based on in vitro Test Methods

Els AdriaensP1, Nathalie Alépée2, Takayuki Abo3, Dan Bagley4, Jason Magby4, Arianna Giusti5, Karsten Mewes6
1 Adriaens Consulting, Belgium
2 L’Oréal R&I, France
3 Kao Corporation, Japan
4 Colgate Palmolive, United States
5 Cosmetics Europe, Belgium
6 Henkel AG & Co. KGaA, Germany
P Presenting author

P-4b-11

DEVELOPMENT OF AN IN VITRO WOUND HEALING MODEL USING LIVE-CELL IMAGING: APPLICATION IN DERMATOLOGY AND COMSETIC FIELDS

Manon BartheP1, Jean-Paul Thénot2, Hanan Osman-Ponchet2
1 PKDERM Laboratories
2 PKDERM SAS
P Presenting author

P-4b-12

A NEW HUMAN IN VITRO SKIN MODEL OF EPIDERMAL BARRIER DAMAGE

Manon BartheP1,2, Véronique M. Braud2, Jean-Paul Thénot3, Hanan Osman-Ponchet3
1 PKDERM Laboratories
2 Université Côte d’Azur, CNRS UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire – France
3 PKDERM SAS
P Presenting author

P-4b-13

Study of the efficacy and safety on in vitro human skin models of a Curcumin emulsion for skin pathologies treatment

Mónica BetanzosP1, Adrian  García1, Senda Basasoro2, Paloma Gómez1, Garazi Berra2, Gartze Mentxaka1, Amaia  García1, Nagore Gabilondo2, Felipe Goñi de Cerio1
1 GAIKER Technology Centre, Basque Research and Technology Alliance (BRTA), Zamudio, Spain
2 Materials & Technologies’ Research Group (GMT), Department of Chemical and Environmental Engineering, Faculty of Engineering of Gipuzkoa, University of the Basque Country, Donostia-San Sebastian, Spain
P Presenting author

P-4b-14

Cutaneous tolerance of personal care products dedicated to babies and adults with sensitive skin:  in vitro/in vivo correlation

Marie-Pierre Gomez-BerradaP1, Hélène Recoules1, Sophie Catoire2, Laetitia Beaudequin2, Adeline Josseaume2, Pierre-Jacques Ferret1
1 Safety Products Department, Pierre Fabre Dermo Cosmetique, Toulouse, France
2 Cell Alternativ, Trosly Breuil, France
P Presenting author

P-4b-15

Retrospective review on in vitro phototoxicity data generated in 3D skin models to support development of new OECD test guideline

Helena KandarovaP1, Hans  Raabe2, Allison  Hilberer2, Neepa Choksi3, Dave  Allen3
1 Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dúbravská cesta 9, 841 04 Bratislava, Slovak republic
2 Institute for In Vitro Sciences, Inc., 30 W. Watkins Mill Road, Suite 100, Gaithersburg, Maryland 20878, United States
3 Integrated Laboratory Systems, Inc., 601 Keystone Park Drive, Suite 800, Morrisville, NC, 27560, United States
P Presenting author

P-4b-16

ESTABLISHMENT OF A COMMENSAL 3D SKIN MODEL FOR STUDYING MICROBIAL MODULATION OF PESTICIDE TOXICITY

Maya KissnerP1, Aline Rosin1, Tewes Tralau2, Lisa Lemoine1
1 German Federal Institute for Risk Assessment, Department Pesticides Safety, Junior Research Group Skin Microbiome
2 German Federal Institute for Risk Assessment, Department Pesticides Safety
P Presenting author

P-4b-17

Determination and Sub-categorization of Ocular Irritants Using the EpiOcular Tissue Model – Prediction Models for Liquids and Solids

Silvia LetasiovaP1, Lenka Hudecova1, Jan Markus1, Yulia  Kaluzhny2, Mitch Klausner2
1 MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
2 MatTek Corporation, Ashlad, MA, USA
P Presenting author

P-4b-18

SAFETY AND EFFICACY TESTING OF COSMETIC PRODUCTS: OVERVIEW OF ESTABLISHED METHODS AND NEW MODELS.

Hanan Osman-PonchetP1, Manon Barthe2, Jean-Paul Thénot1
1 PKDERM SAS
2 PKDERM Laboratories
P Presenting author

P-4b-19

In vitro evaluation of safety profile of a cosmetic ingredient – 4-methoxychalcone

Justyna PopiółP1, Karolina Słoczyńska1, Paulina Koczurkiewicz-Adamczyk1, Dorota Żelaszczyk2, Katarzyna Orzeł2, Katarzyna Wójcik-Pszczoła1, Przemysław  Szafrański3, Patryk Kasza3, Elżbieta Pękala1, Agnieszka Gunia-Krzyżak2
1 Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Jagiellonian University – Medical College
2 Department of Bioorganic Chemistry, Faculty of Pharmacy, Jagiellonian University – Medical College
3 Chair of Organic Chemistry, Faculty of Pharmacy, Jagiellonian University – Medical College
P Presenting author

P-4b-20

Pre-Clinical in vitro assessment of tobacco-free nicotine pouch products

Sarah Jean PourP1, Roman  Wieczorek1, Edgar Trelles Sticken1, Ole  Dethloff1, Liam Simms2, Matthew Stevenson2
1 Reemtsma Cigarettenfabriken GmbH
2 Imperial Brands PLC
P Presenting author

P-4b-21

Skin sensitization of “challenging” compounds: in vitro strategy applied to bio-based ingredients

Mickaël PUGINIERP1, Alicia Roso1
1 Seppic, 50 boulevard National, CS 90020, 92257 La Garenne Colombes Cedex, France
P Presenting author

5a. Toxicokinetics and in vitro – in vivo extrapolation

P-5a-1

In vitro biotransformation of propyl-propane-thiosulfonate (PTSO): Identification and characterization of metabolites

Antonio Cascajosa-LiraP1, Concepción  Medrano-Padial1, Silvia Pichardo1, Alberto Baños2, Enrique Guillamón2, Ángeles  Jos1, Ana M. Cameán1
1 Area of Toxicology. Faculty of Pharmacy. University of de Seville, Profesor García González n°2, 41012 Seville, Spain.
2 DMC Research Center. Camino de Jayena, 82, 18620 Alhendín, Granada, Spain.
P Presenting author

P-5a-2

Preliminary results to the determination of dermal toxicological reference  values for a neurotoxic organophosphorus agent

Marie-Laure CointotP1, Lysiane Champion2, Alan Hubert2, Jennifer Millerioux2, Anne Bossée2
1 French Ministry for Armed Forces
2 French Ministry for Armed Forces
P Presenting author

P-5a-3

Preliminary results to the determination of dermal toxicological reference  values for a neurotoxic organophosphorus agent

Marie-Laure COINTOTP1, Lysiane CHAMPION1, Alan HUBERT1, Jennifer MILLERIOUX1, Anne BOSSE1
1 French Ministry of Armed Forces
P Presenting author

P-5a-4

PBK Modelling Of In Vivo Distribution Kinetics In The ONTOX Project

René GeciP1,2, Alicia Paini2, Stephan Schaller2, Huan Yang2, Lars Küpfer1
1 University Hospital RWTH Aachen, 52074 Aachen, Germany
2 esqLABS GmbH, Hambierich 34, 26683 Saterland, Germany
P Presenting author

5b. In vitro systems to assess respiratory toxicity  

P-5b-1

INHALATION TOXICITY ASSESSMENT OF AN AEROSOLIZED SUNSCREEN PRODUCT WITH AN IN VITRO PULMONARY MODEL

Béatrice BUI1, Marie-Pierre GOMEZ-BERRADAP1, Pierre-Jacques FERRET1
1 Safety Products Department, Pierre Fabre Dermo Cosmetique, Toulouse, FRANCE
P Presenting author

P-5b-2

Novel fully primary human airway epithelium-alveolar macrophages in vitro co-cultures models to study host pathogen interactions

Bernadett  Boda1, Samuel Constant P1
1 Epithelix
P Presenting author

P-5b-3

Utilisation of human 3D bronchial tissues for e-cigarette assessment

Matthew  Stevenson1, Lukasz  CzekalaP1, Roman Wieczorek1, Edgar Trelles Sticken1, Sarah  Jean Pour 1, Fiona  Chapman1, Liam Simms1
1 Imperial Brands PLC
P Presenting author

P-5b-4

Electronic Nicotine Delivery Systems exhibit lower toxicity compared to cigarettes: The Replica Study experience

Massimo CarusoP1,2, Alfio Distefano1, Rosalia Emma1, Sonja Rust3, Konstantinos Poulas4,5, Fahad Zadjali6, Silvia Boffo7, Antonio Giordano7, Vladislav Volarevic8, Konstantinos Mesiakaris4,5, Georgios Karanasios5, Mohammed  Al Tobi6, Najwa Albalushi6, Angelo Canciello7, Aleksandar Arsenijevic8, Aleksandar Ilic8, Tancredi Caruso1, Giuseppe Carota1, Mariarita Spampinato1, Roberta Pulvirenti1, Pietro Zuccarello9, Margherita Ferrante9, Riccardo Polosa10,2, Giovanni Li Volti1,2
1 Department of Biomedical and Biotechnological Sciences, University of Catania, (Italy)
2 Center of Excellence for the Acceleration of Harm Reduction (CoEHAR), University of Catania, (Italy)
3 ECLAT Srl, spin-off of the University of Catania, (Italy)
4 Institute for Research and Innovation, IRIS, Patras Science Park, Patras (Greece)
5 Laboratory of Molecular Biology and Immunology, Department of Pharmacy, University of Patras, Patras (Greece)
6 College of Medicine and Health Sciences, Department of Clinical Biochemistry, Sultan Qaboos University (Oman)
7 Sbarro Institute for Cancer Research and Molecular Medicine, Department of Biology, College of Science and Technology, Temple University (USA)
8 Center for Molecular Medicine and Stem Cell Research, Department of Microbiology and Immunology, Faculty of Medical Sciences University of Kragujevac (Serbia)
9 Department of Medical, Surgical Sciences and Advanced Technologies “G.F. Ingrassia”, University of Catania (Italy)
10 Department of Clinical and Experimental Medicine, University of Catania (Italy)
P Presenting author

P-5b-5

IN VITRO AIR-LIQUID INTERFACE EXPOSURE OF LUNG CELLS TO THERAPEUTIC AEROSOLS FOR PRECLINCAL DRUG DEVELOPMENT

Evelien FrijnsP1, An Jacobs1, Jo Van Laer1, Karen Hollanders1, Sylvie Remy1, Dirk Jochmans2, Sandra Verstraelen1
1 VITO NV, Unit HEALTH, Boeretang 200, 2400 Mol, Belgium
2 REGA Institute KU Leuven, Herestraat 49, 3000 Leuven, Belgium
P Presenting author

P-5b-6

New concepts in inhalation toxicology: The in vitro approach

Tanja HansenP1, Detlef Ritter1, Jan Knebel1, Nico Sonnenschein1, Norman Nowak1, Sylvia Escher1, Katharina  Blümlein1, Katharina Schwarz1
1 Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Hannover, Germany
P Presenting author

P-5b-7

UNDERSTANDING THE TOXICITY OF A BTEX MIXTURE: AIR/LIQUID INTERFACE EXPOSURE OF ORGANOTYPIC LUNG CULTURES

Nour JaberP1, Claude  Emond2, Fabrice  Bray3, Paul  Genevray4, Christian  Rolando3, Fabrice  Cazier4, Sylvain  Billet1
1 UR4492, Unité de Chimie Environnementale et Interactions sur le Vivant, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d’Opale, Dunkerque, France.
2 PKSH Inc, Département de Santé Environnementale et Santé au Travail de l’Ecole de santé publique, Université de Montréal, Canada.
3 Univ. Lille, CNRS, UAR 3290, MSAP, Miniaturisation pour la Synthèse l’Analyse et la Protéomique, F-59 000, Lille, France.
4 CCM, Centre Commun de mesure, Université du Littoral Côte d’Opale, Dunkerque, France.
P Presenting author

P-5b-8

Fine dust toxicity evaluation on uterus using endometrial stem cells

Soo-Rim KimP1,2, Se-Ra Park1,2, Seong-Kwan Kim1,2, In-Sun Hong1,2
1 Laboratory of stem cell research, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 406-840, Republic of Korea.
2 Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 406-840, Republic of Korea.
P Presenting author

P-5b-9

Flavors in e-cigarettes – a tasty hazard?

Selina RinaldiP1, Elke  Pieper1, Nadja Mallock1, Peter Laux1, Andreas Luch1
1 German Federal Institute for Risk Assessment – Department for Chemical and Product Safety
P Presenting author

P-5b-10

Dosimetry in inhalation: an in vitro inhalation model to study the relevance of dose parameters related to particle mass and particle number

Detlef RitterP1, Katharina Schwarz1, Wolfgang Koch1
1 Fraunhofer ITEM
P Presenting author

P-5b-11

Development of alternative in vitro and ex vivo models for testing of inhalable antibiotics

Katharina SchwarzP1, Sabine  Wronski1, Detlef Ritter1, Jan Knebel1, Katharina Bluemlein1, Sven Cleeves1, Nico Sonnenschein1, Laura Mueller1, Monika Niehof1, Katherina Sewald1, Tanja Hansen1, Norman Nowak1, Armin Braun1
1 Fraunhofer Institute for Toxicology and Experimental Medicine
P Presenting author

6a. In vitro methods for safety testing of biopharmaceuticals/biotherapies/vaccines  

P-6a-1

Liver spheroid co-cultures with fresh or cryopreserved hepatocytes and endothelial cells as tool to investigate metabolism and hepatotoxicity

Jana Moer1, David Kwapiszewski1, Anett UllrichP1, Simon Beuck2, Madlen Matz-Soja3, Andrea Zimmermann4
1 Primacyt Cell Culture Technology GmbH
2 A&M Labor Service GmbH
3 Lehrstuhl für Allgemeine Biochemie, Universität Leipzig
4 Hepatobiliäre Chirurgie und viszerale Transplantation, Sächsischer Inkubator für klinische Translation
P Presenting author

P-6a-2

A novel polymer generates cell repellent surfaces and allows 3D cell culture

Anett Ullrich1, Veronique Schwartz2, Dieter RungeP1
1 Primacyt Cell Culture Technology GmbH, Schwerin, Germany
2 Chemovator GmbH, Mannheim, Germany
P Presenting author

P-6a-3

Use of Assay Ready THP-1 derived macrophages to test for pyrogen contamination

Adrian Dittberner1, Lukas FockeP1, Mirta Jacobs1, Karen Hinsch1
1 acCELLerate GmbH
P Presenting author

P-6a-4

Validation of the ToxProfiler reporter assay and its application in mechanistic toxicity testing

Bas ter Braak1, Liesanne Wolters1, Giel Hendriks1, Torben OsterlundP1
1 Toxys B.V.
P Presenting author

P-6a-5

Genotoxicity assessment of potentially mutagenic nucleoside analogues using ToxTracker

Inger Brandsma1, Remco Derr1, Gaonan  Zhang1, Nynke Moelijker1, Giel Hendriks1, Torben Osterlund1
1 Toxys B.V.
P Presenting author

P-6a-6

Exploring the use of spheroid cultures of human liver cells for the mechanistic testing of carcinogenic compounds

Bruna dos Santos Rodrigues1, Prashant KadamP1, Kaat  Leroy1, Sarah  Vanbellingen2, Joeri L.  Aerts2, Mathieu Vinken1
1 Entity of In Vitro Toxicology and Dermato-Cosmetology, Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
2 Entity of Neuro-Aging and Viro-Immunotherapy, Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.
P Presenting author

P-6a-7

In vitro study of the potential of new liposomal delivery systems for future medical applications in inhalation therapies

Juliana Carrillo Romero1,2, Amaia Garcia BilbaoP1, Laura Fernández Méndez2, Adrian Garcia1, Alberto Katsumiti1, Pedro  Ramos Cabrer2,3, Susana  Carregal Romero2,3,4, Felipe Goñi de Cerio1
1 GAIKER Technology Centre, Basque Research and Technology Alliance (BRTA), Zamudio, 48170 Spain
2 Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), San Sebastián, 20014 Spain
3 Ikerbasque, Basque Foundation for Science, Bilbao, 48013 Spain
4 CIBER de Enfermedades Respiratorias (CIBERES), Madrid, 28029 Spain
P Presenting author

P-6a-8

In vitro microenvironment modifies the neurotoxic response of SH-SY5Y cells

Véronique De Conto1, Vaihere Cheung1, Gregory Maubon1, Zied Souguir1, Nathalie Maubon1, Elodie Vandenhaute1, Vincent Berezowski2
1 HCS Pharma
2 Lille Neuroscience and Cognition, U1172
P Presenting author

P-6a-9

THE POTENTIAL OF 3D CELL-BASED PLATFORMS FOR THE SCREENING OF NOVEL DRUG CANDIDATES TARGETING THE HEPATIC PLASMODIUM INFECTION

Francisca ArezP1,2, Diana Fontinha3, Sofia P. Rebelo4,2, Christoph Fischli5,6, Claude Oeuvray7, Manuel  Carrondo4,2, Matthias Rottmann5,6, Thomas Spangenberg7, Catarina Brito4,2, Beatrice Greco7, Miguel  Prudêncio3, Paula M Alves4,2
1 iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal
2 Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
3 Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal
4 iBET,  Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal
5 Swiss TPH, Basel 4051, Switzerland
6 University of Basel, Basel 4003, Switzerland
7 Merck Global Health Institute, Ares Trading S.A., Route de La Verrerie 6, CH-1267, Coinsins, Switzerland, a subsidiary of Merck KGaA, Darmstadt Germany
P Presenting author

P-6a-10

The potential of 3D reconstructed Human intestinal models for biowaiver studies and finished products testing

Rola BARCHAMP1, Eric ANDRES1, Axel HUYARD1, Christophe DINI1
1 Oroxcell, 102 avenue Gaston Roussel, 93230 Romainville, France
P Presenting author

P-6a-11

Mechanistic Investigation of Drug-Induced Liver Toxicity using Human 3D InSight Liver Model

Anna BorgströmP1, Katarzyna Sanchez1, Friederike Wenz1, Natalia  Zapiórkowska-Blumer1, Armin  Wolf 1, Bruno Filippi1
1 InSphero AG, Wagistrasse 27a, 8952 Schlieren, Switzerland
P Presenting author

P-6a-12

Use of 3D human liver microtissues to assess hepatotoxicity of biologics

Reiner ClassP1, Andrea Kiessling1, Breda Twomey1, Milena Mennecozzi1, Monika Kijanska2, Agata Gorecka2
1 UCB Biopharma
2 InSphero AG
P Presenting author

P-6a-13

MTS application as alternative in vitro method to assess the toxicity of veterinary autogenous vaccines produced at Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”: preliminary data.

Claudia ColabellaP1, Lucia Anzalone1, Martina Pellegrini1, Giulio Saveri1, Monica Cagiola1, Antonella Di Paolo1
1 Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, via G. Salvemini 1, 06126 Perugia, Italy
P Presenting author

P-6a-14

In vitro microenvironment modifies the neurotoxic response of SH-SY5Y cells

Véronique DE CONTOP1, Vaihere Cheung1, Gregory  MAUBON1, Zied Souguir1, Nathalie Maubon1, Elodie Vandenhaute1, Vincent Berezowski2
1 HCS Pharma
2 Lille Neuroscience and Cognition, U1172
P Presenting author

P-6a-15

Interplay between in vitro off-target pharmacological promiscuity, cytotoxicity, and in vivo tolerability in rodents to improve the safety profile of anti-malarial drug discovery

Helga GeretsP1, Annie Delaunois1, Alicia Sibony1, Reiner Class1, Teresa De Haro Garcia1, Benoit Laleu2, Martin  Lowe3, Renaud Fleurance1, Marie-Luce Rosseels1, Jean-Pierre Valentin1
1 UCB BioPharma SRL
2 MMV
3 exscientia
P Presenting author

P-6a-16

USEFULNESS OF THE ZEBRAFISH EMBRYO MODEL TO EVALUATE NEW THERAPIES AGAINST MULTIDRUG-RESISTANT BACTERIA

Laura GuzmanP1, Cristina  Marqués1, Elena  Sánchez-López2,3,4, Amanda  Cano2,3,4, Antoni  Camins4,5,6, Yolanda  Cajal2, Miren  Ettcheto4,5,6, Francesc  Rabanal7, Marta  Barenys1,8
1 GRET i Unitat de Toxicologia, Departament de Farmacologia, Toxicologia i Química Terapèutica, Facultat de Farmàcia i  Ciències de l’Alimentació, Universitat de Barcelona (UB), Av. de Joan XXIII, 27-31, 08028 Barcelona, Spain
2 Unitat de Fisicoquímica, Departament de Farmàcia, Tecnologia farmacèutica i fisicoquímica, Facultat de Farmàcia i  Ciències de l’Alimentació, Universitat de Barcelona (UB), Av. de Joan XXIII, 27-31,08028 Barcelona, Spain
3 Institut de Nanociència i Nanotecnologia (IN2UB), Universitat de Barcelona (UB), Av. Diagonal, 64, 08028 Barcelona, Spain.
4 Centro de Investigación Biomédica en Red Enfermedades Neurodegenerativas (CIBERNED), Instituto de Carlos III, Av. Monforte de Lemos, 3-5, 28029 Madrid, Spain.
5 Unitat de Farmacologia, Departament de Farmacologia, Toxicologia i Química Terapèutica, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona (UB), Av. de Joan XXIII, 27-31,08028 Barcelona, Spain
6 Institut de Neurociències, Universitat de Barcelona (UB), Passeig de la Vall d’Hebron, 171, 08035 Barcelona, Spain
7 Unitat de Química Orgànica, Departament de química inorgànica i orgànica, Facultat de Química, Universitat de Barcelona (UB), C/Martí i Franquès, 1-11, 08028 Barcelona, Spain
8 Institut de Recerca en Nutrició i Seguretat Alimentària (INSA), Universitat de Barcelona (UB), Av. Prat de la Riba, 171, 08921 Barcelona, Spain
P Presenting author

P-6a-17

Induction and evaluation of an oxidative stress response in the EpiDermFT in vitro human skin model

Jan MarkusP1, Silvia Letasiova1, Kaitlyn Marengo2, Jon Oldach2, Alex Armento2, Anna Langerveld3, Elisabeth Lehigh3
1 MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
2 MatTek Corporation, Ashland, MA, USA
3 Genemarkers LLC, Kalamazoo, MI
P Presenting author

P-6a-18

hiPSCs and hiPSCs-derived renal proximal tubular cells showed different response to nephrotoxic compounds

Isaac Musong Mboni JohnstonP1, Nicole Schupp1
1 Heinrich Heine University hospital, Institute of Toxicology
P Presenting author

P-6a-19

Low Endotoxin Recovery (LER) effect in pharmaceutical products analyzed with the Monocyte Activation Test (MAT).

Marco MingollaP1,2, Chiara Celli2, Fabrizio Lecce2
1 Università degli Studi di Torino, Molecular Medicine, Torino, Italy
2 Merck KGaA, Istituto di Ricerche Biomediche “A. Marxer” RBM S.p.A., Ivrea, Italy
P Presenting author

P-6a-20

An epidermal model containing melanocytes for skin pigmentation and lightening studies

Marek PuskarP1, Bridget Breyfogle2, Seyoum Ayehunie2, Silvia Letasiova1, Jon Oldach2, Alex Armento2, Mitchell Klausner2
1 MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
2 MatTek Corporation, Ashland, MA, USA
P Presenting author

P-6a-21

PITFALLS OF THE MTT ASSAY AND CYTOTOXICITY EVALUATION OF TRADITIONAL ANTIMALARIALS

Lucia RackovaP1
1 Centre of Experimental Medicine, Slovak Academy of Sciences, Institute of Experimental Pharmacology and Toxicology, Dubravska cesta 9, 841 04 Bratislava, Slovakia
P Presenting author

P-6a-22

Long-term upregulation in drug metabolization and hepatic gene expression in primary human hepatocytes (PHH) after exogenous exposure to human intestinal microbiome secretome peptides

Natalia Sánchez-RomeroP1,2, Pilar  Sainz de la Maza Arnal1,2, Maria Jesús Lozano Limones1,2, Mario  Fernández Sanz2, Álvaro  Blanes Rodríguez2, Sandra  Meliton Barbancho2, Pedro Baptista2,3,4,5
1 Cytes Biotechnologies
2 Health Research Institute of Aragón (IIS Aragón)
3 Department of Biomedical and Aerospace Engineering, Carlos III University of Madrid
4 ARAID Foundation
5 CIBERehd
P Presenting author

P-6a-23

Novel in vitro approaches in safety evaluation of cemtirestat

Marta Šoltésová PrnováP1,2, Peter Pôbiš1, Marek Lepáček1, Lucia Račková1, Jana Viskupičová1, Tea Lanišnik Rižner3, Sreeparna Banerjee4, Helena Kanďárová5, Milan Štefek5
1 Institute of Experimental Pharmacology and Toxicology, CEM, Slovak Academy of Sciences, Bratislava, Slovakia
2 Faculty of informatics and information technologies Slovak University of Technologies in Bratislava, Slovakia
3 Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Slovenia
4 Department of Biological Sciences, Middle East Technical University, Ankara, Turkey
5 nstitute of Experimental Pharmacology and Toxicology, CEM, Slovak Academy of Sciences, Bratislava, Slovakia
P Presenting author

P-6a-24

Drug-induced QTc prolongation and Torsades de Pointes: evolving ICH S7B in light of emerging in silico, in vitro and in vivo data and implication for drug development

Jean-Pierre VALENTINP1, Derek LEISHMAN2, Hugo VARGAS3
1 UCB Biopharma, Braine l’Alleud, Belgique
2 Eli-Lilly, Indianapolis, USA.
3 Amgen, California, USA
P Presenting author

6b. Knowledge sharing and education  

P-6b-2

Ukrainian National 3Rs Center. An effective tool to advance the 3Rs principles by focusing on their scientific impacts and benefits

Nataliia BubaloP1, Serhii Kolesnyk1, Petro Zhminko1, Marina Zinovieva1, Yana  Kolianchuk1, Inna Rashkivska1
1 L.I.Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety Ministry of Health, Ukraine
P Presenting author

P-6b-3

Reproducibility in Cell Culture: Replacing Fetal Bovine Serum

Tilo WeberP1, Kristina Wagner 1, Jessica Rosolowski1, Joachim Wiest2
1 Animal Welfare Academy of the German Animal Welfare Federation, Neubiberg, Germany
2 cellasys GmbH, Kronburg, Germany
P Presenting author

7a. Developmental Neurotoxicity (DNT)  

P-7a-1

Developmental toxicity assessment of nanoparticles: The importance of indirect placenta-mediated toxicity mechanisms

Battuja Dugershaw-Kurzer1,2, Patrycja  Nowak-Sliwinska3,4, René Hornung5, Arjan Griffioen6, Judy R. van Beijnum6, Stefan Masjosthusmann7, Ellen Fritsche7, Tina Buerki-ThurnherrP1
1 Laboratory for Particles Biology Interactions, Swiss Federal Laboratories for Materials Science and Technology (Empa), St. Gallen, Switzerland
2 Laboratory of Toxicology, Department of Health Sciences and Technology, ETH Zurich, Zurich, Switzerland
3 Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland
4 Molecular Pharmacology Group, School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland
5 Department of Gynaecology and Obstetrics, Kantonsspital St.Gallen, St. Gallen, Switzerland
6 Angiogenesis Laboratory, Department of Medical Oncology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands
7  IUF – Leibniz Research Institute for Environmental Medicine, Duesseldorf, German
P Presenting author

P-7a-2

DEVELOPMENTAL NEUROTOXICITY OF ACRYLAMIDE AND ITS METABOLITE GLYCIDAMIDE IN A HUMAN MIXED CULTURE OF NEURONS AND ASTROCYTES UNDERGOING DIFFEFRENTIATION

Oddvar MyhreP1, Malene Lislien1, Jørn A Holme2, Hubert Dirven1, Ragnhild E Paulsen3, Inger Margit Alm1, Ellen Skarpen4, Vigdis Sørensen4, Peter Macko5, Francesca Pistollato5, Nur Duale1, Anna J Lauvås1
1 Department of Chemical Toxicology, Norwegian Institute of Public Health, Oslo, Norway.
2 Department of Air Pollution and Noise, Norwegian Institute of Public Health, Oslo, Norway.
3 Department of Pharmacy, University of Oslo, Norway
4 Core Facility for Advanced Light Microscopy, Institute for Cancer Research, Oslo University Hospital, Norway
5 European Commission, Joint Research Centre (JRC), Ispra, Italy
P Presenting author

P-7a-3

In vitro study of fumonisin B1 and ochratoxin A on undifferentiated SH-SY5Y cells and contribution of beetroot in alleviating toxic effects

Raquel Penalva-OlcinaP1, Mónica  Fernández-Franzón1, Ana Juan-García1
1 Universitat de València
P Presenting author

P-7a-4

APPLIYING NAMS TO SAFETY EVALUATION OF HERBAL PRODUCTS CONSUMED BY CATALAN PREGNANT AND LACTATING WOMEN: INTERVIEW-BASED CONSUMPTION SURVEY AND DEVELOPMENTAL NEURO/TOXICITY EVALUATION IN ZEBRAFISH EMBRYOS

Noelia G RomeroP1, Elisabet  Teixidó1,2, Nicole  Feliu1, Salvador  Cañigueral3, Jesús  Gómez-Catalán1,2, Marta  Barenys1,2
1 GRET and Toxicology Unit, Department of Pharmacology, Toxicology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Av. Joan XXIII 27 31, Barcelona, Spain.
2 Institute of Nutrition Research and Food Safety (INSA UB), University of Barcelona, Barcelona, Spain.
3 Unitat de Farmacologia, Farmacognòsia, i Terapèutica, Facultat de Farmàcia, i Ciències de l’Alimentació, Universitat de Barcelona, Av. Joan XXIII  27-31, 08028 Barcelona, Spain.
P Presenting author

8a. In vitro methods for safety assessment of medical devices

P-8a-1

Optimization of skin sensitization testing strategy in vitro for medical device extracts

Lada Svobodova1,2, Kristina KejlovaP1, Alena Vlkova1, Marian Rucki1, Dagmar Jirova1, Hana Kolarova2
1 National Institute of Public Health Prague
2 Faculty of Medicine and Dentistry, Department of Medical Biophysics, Palacký University
P Presenting author

P-8a-2

In vitro method for quantitative potency assessment of skin sensitizers during development of novel materials for intended use in medical devices

Ulrika Mattson1, Karla Lienau2, David Waeckerlin2, Robin Gradin1, Rose-Marie Jenvert1
1 SenzaGen AB
2 Sonova AG
P Presenting author

P-8a-3

Development, pre-validation and validation of the EpiDerm in vitro skin irritation protocol for the medical devices extracts

Silvia LetasiovaP1, Jan  Markus1, Bridget  Breyfogle2, Michael A.  Bachelor2, Helena  Kandarova3
1 MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
2 MatTek Corporation, Ashland, MA, USA
3 Centre of Experimental Medicine SAS, Slovak Academy of Sciences, Bratislava, Slovakia
P Presenting author

P-8a-4

Bio-compatibility assessment of medical devices using reconstructed in vitro 3D human cornea-like tissue model

Peter  PôbišP1, Lucia Milec1, Zuzana Straková1, Helena Kandarova1
1 Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, 841 04 Bratislava, Slovakia
P Presenting author

8b. In vitro COVID-19 research / Lung and cardiovascular models

P-8b-1

COMPARISON OF EXPRESSION PROFILE OF SARS-COV-2 KEY RECEPTORS IN SKIN AND LUNG IN VITRO MODELS

Manon BartheP1,2, Véronique M. Braud2, Jean-Paul Thénot3, Hanan Osman-Ponchet3
1 PKDERM Laboratories
2 Université Côte d’Azur, CNRS UMR7275, Institut de Pharmacologie Moléculaire et Cellulaire – France
3 PKDERM SAS
P Presenting author

P-8b-2

INTESTINAL ADVERSE OUTCOMES IN COVID-19: CURRENT EVIDENCE AND UNCERTAINTIES USING THE ADVERSE OUTCOME PATHWAY FRAMEWORK.

Laure-Alix ClerbauxP1, Julija Fillipovska2, Amalia Muñoz3, Mauro Petrillo4, Helena Soares5, Sally Mayasich6, Maria-Joao Amorim7, Lucia Grenga8
1 European Commission, Joint Research Centre (JRC), Ispra, Italy
2 Independent scientist, Ohrid, North Macedonia
3 European Commission, Joint Research Centre (JRC), Geel, Belgium
4 Past affiliation: European Commission, Joint Research Centre (JRC), Ispra, Italy. Current affiliation: Seidor Italy srl, Milano, Italy
5 Human Immunobiology and Pathogenesis Group, CEDOC, NOVA Medical School | Faculdade de Ciências Médicas, NOVA University of Lisbon, Lisbon, Portugal
6 University of Wisconsin-Madison Aquatic Sciences Center at US EPA, Duluth, MN, USA
7 Instituto Gulbenkian de Ciência, Lisbon, Portugal
8 Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé, Paris, France
P Presenting author

P-8b-3

COVID-19 through the lens of the Adverse Outcome Pathways: What can we learn from the view on the relationship between ACE2 dysfunction and its interaction with SARS-COV2

Julija FilipovskaP1,2
1 Independent Scientist, Ohrid, North Macedonia
2 The work was inspired and performed as part of the CIAO project (https://www.ciao-covid.net/)
P Presenting author

P-8b-4

Potential role of quercetin in modulating SARS-CoV-2-induced cytokine storm

Elodie OLIVIERP1, Mélody DUTOT1,2, Patrice RAT1
1 Laboratoire de Chimie Analytique et Toxicologie Expérimentale Toxicologie Cellulaire (UMR CNRS 8038), Faculté de Pharmacie de Paris – Université de Paris
2 Yslab, 2 rue Félix Le Dantec, 29000 Quimper, France
P Presenting author

P-8b-5

ESTROGENIC ACTIVITY RELATED TO THE PRESENCE OF SARS-COV-2 IN WASTEWATER

Francesca RispoP1, Lorenzo  Dondero1, Giulia  De Negri Atanasio1, Giorgia  Allaria1, Francesca Tardanico 1, Stefano Rosatto2, Marta Bellisomi2, Giulia Palumbi2, Irene Tomesani2, Alberto Izzotti3, Elena Nicosia2, Elena Grasselli1
1 Department of Earth, Environment and Life Science, University of Genoa, Genova, Italy
2 ARPAL Liguria, Italy
3 Department of Experimental Medicine, University of Genoa, Genova, Italy
P Presenting author

Models and methods 

P-MODELS-1

APPLICATION OF BENCHMARK DOSE APPROACH FOR ASSESSMENT OF EFFECTS ON RED BLOOD CELLS REVEALED DURING NON-CLINICAL TOXICITY STUDY OF DRUG CANDIDATE

Mykola Prodanchuk1, Zhminko Peter1, Maryna ZinovievaP1, Serhii Kolesnyk1
1 L.I.Medved’s Research Center of Preventive Toxicology, Food and Chemical Safety, Ministry of Health, Ukraine, Kyiv
P Presenting author

P-MODELS-2

IN VITRO TRANSCRIPTIONAL BIOMARKERS INVOLVED IN LIVER STEATOSIS AND MIXTURE EFFECTS OF HEPATOTOXIC PESTICIDES THROUGH AN ADVERSE OUTCOME PATHWAY-BASED APPROACH

Efrosini Katsanou1, Anastasia  SpyropoulouP1, Petros Batakis1, Dajana Lichtenstein2, Claudia Luckert2, Albert Braeuning2, Alfonso Lampen2, Kyriaki Machera1
1 Laboratory of Pesticides’ Toxicology, Department of Pesticides Control and Phytopharmacy, Benaki Phytopathological Institute, Athens, Greece
2 German Federal Institute for Risk Assessment, Dept. Food Safety, Berlin, Germany
P Presenting author

P-MODELS-3

Thermodynamic study on efficient removal of Cr(III), Cu(II) and Cd(II) heavy metal ions by SnO2/MWCNT nanocomposite.

Shima Mohamad Khani1, Mahdieh Bazargani2, Nikoo Hosseini1
1 Islamic Azad University, Science and Research Branch ,Tehran ,Iran.
2 Sharif University of Technology,Tehran,Iran.
P Presenting author

P-MODELS-4

PANORAMIX: Providing risk assessments of complex real-life mixtures for the protection of Europe’s citizens and the environment

Anne Marie Vinggaard1, Marja  Lamoree2, Beate  I. Escher3, Jean-Philippe Antignac4, Martin  Scholze5, Tina Kold Jensen6, Matthias Herzler7, Marc Audebert8, Timo Hamers2, Andreas  Kortenkamp5, François  Busquet9, Matteo PiumattiP9, Gaud  Dervilly4, Maria  João Valente1, Ronan  Cariou4, Solène  Moteau4, Michael  Oelgeschläger7, Kostja  Renko7, Sebastian  Schmeisser7, Dieter  Maier10, Lone  Ladegaard Laursen11
1 National Food Institute, Technical University of Denmark, Kemitorvet Building 202, 2800 Kgs. Lyngby, Denmark
2 Vrije Universiteit, Department Environment & Health, De Boelelaan 1108, 1081 HZ Amsterdam, the Netherlands
3 Department of Cell Toxicology, Helmholtz Centre for Environmental Research – UFZ, Permoserstr. 15, DE-04318 Leipzig, Germany; Environmental Toxicology, Center for Applied Geoscience, Eberhard Karls University Tübingen, Schnarrenbergstr. 94-96, DE-72076 Tübingen, Germany.
4 Oniris, INRAE, LABERCA, Nantes, France
5 Brunel University London, Institute of Environment, Health and Societies, Kingston Lane, Uxbridge, Middlesex UB8 3PH, United Kingdom
6 Dep of Environmental Medicine, University of Southern Denmark, Denmark
7 German Federal Institute for Risk Assessment (BfR), Department Chemicals and Product Safety, Max-Dohrn-Str. 8-10, 10589 Berlin, Germany
8 PrediTox, Toulouse, France
9 Altertox Academy, Brussels, Belgium
10 Biomax Informatics, Planegg, Germany
11 Faculty of Health Science, University of Southern Denmark, Denmark
P Presenting author

P-MODELS-5

IN VITRO MUTAGENICITY AND GENOTOXICITY OF PURE ANATOXIN-A

Cristina Plata-Calzado1, Concepcion Medrano-Padial1, Leticia Diez-Quijada1, Ana I Prieto1, Ana M Camean1, Angeles JosP1
1 Area of Toxicology. Faculty of Pharmacy. Universidad de Sevilla. Spain
P Presenting author

P-MODELS-6

Mutagenenicity and genotoxicity evaluation of reduced graphene oxide by the mouse lymphoma assay and standard and enzyme- modified comet assays

Oscar Cebadero-Dominguez1, Concepcion Medrano-Padial1, Maria Puerto1, Ana M Camean1, Angeles JosP1
1 Area of Toxicology. Faculty of Pharmacy. Universidad de Sevilla. Spain
P Presenting author

P-MODELS-7

GLIMPSES INTO IN VITRO TESTING OF MYCOTOXIN MIXTURES: INTERACTION BETWEEN TOXINS AND THEIR METABOLIC ACTIVATION

Mohammad Abdulkader AkbarshaP1, Loganathan Gayathri2, Dharumadurai Dhanasekaran2, Albert P Li3
1 Mahatma Gandhi-Doerenkamp Centre (MGDC), Bharathidasan University, Tiruchirappalli 620024, India. Present address: National College (Autonomous), Tiruchirappalli 620001, India
2 Department of Microbiology, Bharathidasan University, Tiruchirappalli 620024, India
3 In Vitro ADMET Laboratories LLC, 9221 Rumsey Road, Columbia, MD 21045, USA
P Presenting author

P-MODELS-8

Regulation of cholesterol transport and metabolism by tumor necrosis factor alpha in a human in vitro blood-brain barrier model

Rodrigo Azevedo LoiolaP1, Shiraz Dib1, Lucie Dehouck1, Emmanuel Sevin1, Jens Pahnke2, Fabien Gosselet1
1 Université d’Artois – France
2 University of Oslo, Norway
P Presenting author

P-MODELS-9

Development of an adverse outcome pathway for kidney tubular necrosis

Devon BarnesP1, Alasdair Irvine1, Alessio Gamba2, Luiz Carlos Maia Ladeira2, Bernard Staumont2, Huan Yang3, Inger-Lise Steffensen4, Liesbet Geris2,5,6, Manoe Janssen1, Rosalinde Masereeuw1
1 Utrecht Institute for Pharmaceutical Sciences, Utrecht University
2 Biomechanics Research Unit, GIGA In Silico Medicine, University of Liège
3 esqLABS
4 Department of Food Safety, Norwegian Institute of Public Health
5 Skeletal Biology and Engineering Research Center
6 Biomechanics Section, Department of Mechanical Engineering
P Presenting author

P-MODELS-10

Neurotoxic effect of potential countermeasures in case of nerve agent poisoning

Tena ČadežP1, Zrinka Kovarik1
1 Institute for Medical Research and Occupational Health, Zagreb, Croatia
P Presenting author

P-MODELS-11

New Salmonella strains resistant to sulfonylurea and  triazole-pyrimidine herbicides and their use in the Ames test

Olga EgorovaP1, Nataliya Suzina2,3, Nataliya Ilyushina1
1 The Federal Budgetary Establishment of Science «Federal Scientific Center of Hygiene named after F.F. Erisman» of Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2 G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
3 Federal Research Center «Pushchino Center for Biological Research of the Russian Academy of Sciences»
P Presenting author

P-MODELS-12

BPS DISPLAYS SIMILAR PHENOTYPIC AND METABOLIC RESPONSES TO BPA IN HEPG2 AND INS-1E CELLS

Lara Ferreira AzevedoP1,2, Mauro Miguel Masiero2, Sarah Cherkaoui2, Maria Fernanda Hornos Carneiro 1,3, Fernando  Barbosa Júnior1, Nicola Zamboni2
1 Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, Brazil
2 Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
3 Facultad de Química y de Farmacia, Campus San Joaquín Pontificia Universidad Católica de Chile, Santiago, Chile
P Presenting author

P-MODELS-13

Comparison of a heated tobacco stick product and a combustible cigarette: chemical analysis and in vitro toxicological evaluation

Tsuneo HashizumeP1, Shinkichi Ishikawa1, Shigeaki Ito1, Toshiro Fukushima1
1 Japan Tobacco Inc.
P Presenting author

P-MODELS-14

A High Content Analysis approach for a better detection of bacterial factors involved in the virulence of Bacillus cereus and Clostridium perfringens Strains

Kevin HogeveenP1, Rachelle Lanceleur1, Abdelrahim Abakabir-Mahamat1, Olivier Firmesse1, Valérie  Fessard1
1 French Agency for Food, Environmental and Occupational Health and Safety
P Presenting author

P-MODELS-15

Investigation of toxicokinetic and toxikodynamic mixture effects within plant protection products using new approach methodologies: a case study of two products containing tebuconazole + prothioconazole and cypermethrin + piperonylbutoxide

Mawien KaracaP1,2, Christian Tobias Willenbockel1, Tewes Tralau1, Denise Bloch1, Philip Marx-Stoelting1,2
1 German Federal Institute for Risk Assessment
2 Technical University of Berlin
P Presenting author

P-MODELS-16

CHRONIC LOW-DOSE EXPOSURE TO DIBUTYL PHTHALATE AFFECTS NO PRODUCTION, CELL MIGRATION, AND ANGIOGENESIS IN HUMAN ENDOTHELIAL CELL LINE EA.HY926

Dunja KokaiP1, Bojana Stanic1, Biljana Tesic1, Dragana Samardzija Nenadov1, Kristina Pogrmic-Majkic1, Svetlana Fa Nedeljkovic1, Nebojsa Andric1
1 University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Serbia
P Presenting author

P-MODELS-17

Mixture effects of extracts from environment, food and blood on neurite outgrowth compared to cytotoxicity in SH-SY5Y cells

Jungeun LeeP1, Marc Audebert2, Jean-Philippe Antignac3, Laure Khoury4, Maria König1, Marja Lamoree5, Maria Margalef Jorne5, Solene Motteau3, Chrstina Rørbye6, Rita Schlichting1, Anne-marie Vinggaard7, Maria João Valente7, Beate Escher1
1 Department of Cell Toxicology, UFZ–Helmholtz Centre for Environmental Research, Leipzig, Germany
2 INRAE UMR1331 TOXALIM, France
3 Oniris, INRAE, Laboratoire d’Etude des Résidus et Contaminants dans les Aliments, 44300 Nantes, France
4 Preditox, Toulouse, France
5 Department Environment & Health, Faculty of Science, Vrije Universiteit Amsterdam, The Netherlands
6 Hvidovre Hospital, Denmark
7 National Food Institute, Technical University of Denmark, Lyngby, Denmark
P Presenting author

P-MODELS-18

Connexin-based channel activity is not specifically altered by hepatocarcinogenic chemicals

Kaat LeroyP1, Axelle Cooreman1, Raf Van Campenhout1, Bruno  Cogliati2, Mathieu Vinken1
1 Entity of In vitro Toxicology and Dermato-Cosmetology, Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel
2 Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo
P Presenting author

P-MODELS-19

STUDY OF CITRININ TOXIC EFFECTS ON A 3D NEUROBLASTOMA MODEL: CYTOTOXICITY, OXIDATIVE STRESS AND CELL DEATH

Francisco J.  Martí-QuijalP1, Noemi Torriero2,3, Francisco J.  Barba1, María José  Ruiz1, Maria Rosaria  Esposito2,3, Elisa Cimetta2,3
1 Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100, Valencia, Spain
2 Department of Industrial Engineering (DII), University of Padua, Via Marzolo 9, 35131, Padova, Italy
3 Fondazione Istituto di Ricerca Pediatrica Città Della Speranza (IRP) – Lab BIAMET, Corso Stati Uniti 4, 35127, Padova, Italy
P Presenting author

P-MODELS-20

THE ROLE OF ENVIRONMENTAL CONTAMINANTS DETECTABLE IN WASTE OF ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) PLANTS IN A549 AND HEPG2 CELL LINES – THE VAISAL PROJECT

Milena MikhailP1, Alessandra Di Veroli2, Anton  Vremere1, Stefano Di Bona2, Francesca Buiarelli3, Giulia Simonetti3, Patrizia Di Filippo4, Donatella Pomata4, Carmela Riccardi4, Franco Lucarelli5, Giulia Pazzi5, Roberta Galarini6, Carolina Barola6, Simone Moretti6, Fabiola Paoletti6, Laura Goracci2, Gabriele Cruciani2, Stefano Lorenzetti7
1 ISS – Istituto Superiore di Sanità, Department of Food Safety, Nutritionn and Veterinary Public Health
2 University of Perugia, Department of Chemistry, Biology and Biotechnology
3 University Sapienza of Rome, Department of Chemistry
4 INAIL-DIT
5 University of Florence, Department of Physics
6 Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche ”Togo Rosati”
7 ISS – Department Food Safety, Nutrition and Veterinary Public Health
P Presenting author

P-MODELS-21

PK-driven drug test for the evaluation of the efficacy of anti-cancer treatment regimens

Andrey PoloznikovP1, Martin Schumacher1, Sergey Nikulin1
1 MimiQ GmbH
P Presenting author

P-MODELS-22

INTEGRATED ENVIRONMENTAL AND HUMAN IN VITRO TOXICITY APPROACH FOR DINOFLAGELLATES

Cristina ScaroneP1, Susanna Alloisio2, Francesco Misurale1, Valentina  Giussani3, Laura Pezzolesi4, Rossella Pistocchi4, Antonio Novellino2, Aldo Pagano1, Anna Maria Bassi1
1 Department of Experimental Medicine- University of Genoa, Italy
2 ETT Spa, Italy
3 ARPAL Liguria, Italy
4 University of Bologna, Italy
P Presenting author

Other – Nanoparticle

P-NANO-1

ASSOCIATION OF DOXORUBICIN AND pH-SENSITIVE NANOPARTICLES CONTAINING AN ORGANOSELENIUM COMPOUND AS AN INNOVATIVE APPROACH TO SENSITIZE MDR CELLS: AN IN VITRO STUDY

Leticia B Macedo1, Daniele R Nogueira-Librelotto1, Daniela Mathes1, Raquel Mello da Rosa2, Oscar E Dorneles Rodrigues2, Clarice M Bueno Rolim1, M Pilar VinardellP3
1 Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil
2 Programa de Pós-Graduação em Química, Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
3 Departament de Bioquimica i Fisiologia, Facultat de Farmacia i Ciències de l’Alimentació, Universitat de Barcelona, Barcelona, Spain
P Presenting author

P-NANO-2

Toxicity of polystyrene micro- and nano-plastics in human A549 lung cells

Marta Pennati1, Judith Desmet1, Tiantian Wang1, Mahboubeh Hosseinzadeh1, Cinta PorteP1
1 IDAEA-CSIC
P Presenting author

P-NANO-3

NEUROTOXICITY ASSESSMENT OF Cd/Se QUANTUM DOTS NANOPARTICLES IN T98G HUMAN GLIOBLASTOMA CELLS THROUGH A TRANSCRIPTOMIC APPROACH

Encarnación Fuster1, Héctor Candela1, Jorge Estévez1, Eugenio Vilanova1, Miguel A. SogorbP1
1 Instituto de Bioingeniería, Universidad Miguel Hernández de Elche, Avenida de la Universidad s/n, 03202 Elche, Spain
P Presenting author

P-NANO-4

New development for studying the genotoxicity of nanomaterials in liver cell models

Julia VARET1, Audrey BARRANGER1, Camille CROCHET1, Sylvie HUET1, Ludovic LE HEGARAT1, Valérie FESSARDP1
1 Anses, Fougères laboratory, Toxicology of contaminants unit
P Presenting author

P-NANO-5

INVOLVEMENT OF OXIDATIVE STRESS AND CALCIUM SIGNALING IN NICKEL OXIDE NANOPARTICLES – INDUCED ALTERATIONS IN HUMAN PULMONARY ARTERY ENDOTHELIAL CELLS

Ophélie GermandeP1,2, Sabrina Lacomme3,2, Etienne Gontier3,2, Jean – François Quignard1,2, Roger Marthan1,2,4, Magalie Baudrimont5,2, Christelle Guibert1,2, Juliette Deweirdt *1,2, Isabelle Baudrimont *    *equal contribution6,7
1 INSERM U 1045, CRCTB
2 Université de Bordeaux
3 BIC, UMS 3420 CNRS – US4 INSERM
4 CHU de Bordeaux
5 UMR EPOC 5805
6 INSERM U 1045, CRCTB 
7 Université de Bordeaux 
P Presenting author

P-NANO-6

Cytotoxicity of nanomixtures: effects of silver and polystyrene nanoparticles on human macrophages

Krunoslav IlicP1, Nikolina Kalcec1, Lucija  Krce2, Ivica  Aviani2, Petra Turčić3, Ivan Pavičić1, Ivana Vinkovic Vrcek1
1 Institute for Medical Research and Occupational Health
2 Faculty of Science, University of Split
3 Faculty of Pharmacy and Biochemistry, University of Zagreb
P Presenting author

P-NANO-7

Erythrocyte photoassay as a preliminary tool to evaluate potential photoprotective activity of guarana-loaded nanosomes

Adriana Solange MaddalenoP1, Isabel Roggia2, Patrícia Gomes2, Maria Pilar Vinardell1, Montserrat Mitjans1
1 Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, Universitat de Barcelona, Barcelona, Spain
2 Post Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil
P Presenting author

P-NANO-8

Exposure to polystyrene micro- and nano-plastics modifies the lipidome of zebrafish liver cells.

Tiantian WangP1, Cinta Porte1
1 IDAEA-CSIC
P Presenting author