Posters

Instructions for Poster Presenters

Please note that unlike what is written in the instructions for poster presentations, you will not be required to stand next to your poster during coffee and lunch breaks. There will be two dedicated poster presentation sessions:

Even poster numbers (not abstract numbers!) will present on……. during Poster Session II

Odd poster numbers will present on ……. June during Poster Session III

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Poster print service:

We offer a convenient local print service through Exposale for all your poster printing needs.

Pricing:

• The cost for print and onsite delivery is €…. excluding VAT if ordered before the deadline of ……..
• After …… please ask a confirmation that the poster can be still printed depending on date of receiving the print file. The price for late poster printing is €….. excluding VAT.

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Preparing Print Files for Large Format Prints:

To ensure optimal results, please adhere to the following guidelines when preparing your print files:

• All posters must be designed in portrait orientation.
• Poster format should be A0 (width: 84.1cm / 33.1in; height: 118.9cm / 46.8in).
• Accepted file type: standard print PDF.
• Ensure all print files are ideally 1:1 scale.
• Name your print files descriptively for easy identification.
• Convert all fonts into curves to avoid font compatibility issues.
• Ideal bitmap resolution is 100-150 DPI at final size; additionally, please keep individual file sizes under 100MB if possible.
• Include bleeds and correct crop marks for optimal printing.
• Use/send files in CMYK color space only.

Contact Information:

To order your printed poster or for any inquiries, please contact Impreso:

• Contact persons: Hai Harings & Nico Eerens
• Email: info@impreso.nl

TOPICS

Bio-engineering, stem cells and disease models
Cardiotoxicity and cardiac efficacy models
Case studies for successful use and implementation of complex in vitro models
Challenges in cosmetics safety
Computational toxicology – in silico modelling, read-across, artificial intelligence and machine learning
ECHA, EMA and EFSA perspectives on the use of NAMs
Funding the development, validation and implementation of NAMs
In vitro and in silico methods for safety assessment of medical devices
In vitro systems to assess respiratory toxicity
Knowledge sharing and education
Local toxicity testing (safety and efficacy)
Models, biomarkers and assays for developmental toxicity
Models, biomarkers and assays for endocrine disruption
Models, biomarkers and assays for systemic and immune toxicity
Non-animal methods for safety testing of biopharmaceuticals/biotherapies/vaccines
Organ-on-a-chip & Microphysiological Systems
Other
Regulatory considerations for complex NAMs
Role of 3R centres in the dissemination and implementation of NAMs
Serum-free approaches
Toxicokinetics and in vitro – in vivo extrapolation

Go back

Bio-engineering, stem cells and disease models 

P1

Advanced lung organoids mimicking proximal and distal regions for viral infection and drug screening

PhD. Alicia Reyes Valenzuela^P1,2, PhD. Mark  Turner³, PhD. Silvia Vidal², PhD. Luc Mongeau^2
1 PulmonaVat
² McGill University
³ Health Canada
^P Presenting author

P2

Assessment of hepatic fibrosis through impedance technology using a three-dimensional cell model

Ye Jin Do^P1,2, Soojin Kim¹, Jung-Hwa Oh^1,2, Seokjoo Yoon^1,2
1 Korea Institute of Toxicology, Republic of Korea
² University of Science and Technology, Republic of Korea
^P Presenting author

P3

Characterization of stage I retinal organoids: a proteomic approach

Sara  Tirendi^1,2, Anna Maria Bassi^P1,2, Mariapaola  Nitti¹, Nicole Grinovero³, Sonia  Scarfi^4,2, Serena  Mirata^4,2, Mario Passalacqua^1,2, Fabrizio Loiacono⁵, Andrea  Petretto³, Stefania Vernazza^1,2
1 Department of Experimental Medicine (DIMES), University of Genoa, Italy. 
² Italian Center3R
³ Core Facilities – Clinical Proteomics and Metabolomics, IRCCS Istituto Giannina Gaslini, Genoa, Italy
⁴ Department of Earth, Environment and Life Sciences (DISTAV), University of Genoa, Italy
⁵ IRCCS Ospedale Policlinico San Martino, Genoa, Italy
^P Presenting author

P4

Chicken-derived R-spondin1, Wnt3a, Nrg1 and FGF1 allow branched growth of embryonal and adult chicken intestinal organoid cultures

Jean Paul Ten Klooster¹, Kitty Van Summeren¹, Raymond Pieters^P1
1 University of Applied Sciences Utrecht (UASU)
^P Presenting author

P5

Bridging complexity and accessibility: A simplified organoid-derived cell model for advanced in vitro applications

Kitty van Summeren^P1, Raymond Pieters^1,2, Jean Paul ten Klooster^1
1 Innovative Testing in Life Sciences and Chemistry (INT), University of Applied Sciences Utrecht (UASU)
² Institute for Risk Assessment Sciences, Population Health Sciences Division, Utrecht University, Utrecht
^P Presenting author

P6

Chronic pain in a dish: modelling molecular pain mechanisms for transforming toxicity testing and drug development

Kathrin Laemmerhirt^P1, Lisa Maria Haiber¹, Bettina Seeger^1
1 Institute for Food Quality and Food Safety, Research Group Food Toxicology and Alternative/Complementary Methods to Animal Experiments, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany
^P Presenting author

P7

Reproducible generation of robust human iPSC-derived alveolar macrophage, for in vitro lung toxicology

Shogo Nagata^P1, Erik Johansson¹, Ariko Fuke¹, Masako Kishihata¹, Kensuke Matsumoto¹, Arun Kumar¹, Harry Choi¹, Toshio Suzuki¹, Tetsuharu Nagamoto¹, Yuki Yamamoto^1
1 HiLung Inc.
^P Presenting author

P8

Development of an in vitro assessment for drug-induced peripheral neuropathy using hiPSC-derived sensory neurons

Malina Graf^P1, Frida Eriksson¹, Paul Fitzpatrick^1
1 AstraZeneca, R&D BioPharmaceuticals, Clinical Pharmacology & Safety Sciences
^P Presenting author

P9

Scalable and well-defined human CNS co-culture platforms suitable for modelling excitatory-inhibitory neuron imbalances in drug discovery and safety studies

Tony Oosterveen^P1, Mitzy Rios de Anda¹, Tulin Tatar Ozkan¹, Sarah Hussain¹, Aurora Veteleanu¹, Stefan Milde¹, Mariangela Iovino¹, Dario Magnani¹, Tom Harris-Brown¹, Rebecca Northeast¹, Inês Ferreira¹, Amanda Turner¹, Will Bernard^1
1 bit.bio
^P Presenting author

P10

Comparative study of CYP450 gene regulation in human pluripotent stem cell-derived liver 2D cell and 3D organoid models

Ji-Woo Kim¹, Seongyea Jo¹, Ji Hyeon Ryu¹, Haneul Noh¹, Han-Jin Park¹, Hyemin Kim^P1
1 Korea Institute of Toxicology
^P Presenting author

P11

Application of scalable hiPSC-derived liver organoids for chemical hazard screening

Ji-Woo Kim Kim¹, Seongyea  Jo¹, Ji Hyeon Ryu¹, Haneul  Noh¹, Hyemin  Kim¹, Han-Jin Park^P1
1 Korea Institute of Toxicology
^P Presenting author

P12

Engineering a human hiPSC-derived 3D liver model to predict early innate immune toxicity to AAV gene therapy

Isabella Ramella Gal^P1,2, Inês Pereira Correia^1,2, Francisca Arez^1,2, Maria França Pereira^1,2, Inês Saldanha^1,2, Giacomo  Domenici^1,2, Sofia Fernandes^1,2, Catarina Freitas^1,2, Paula Marques Alves^1,2, Udo Maier³, Ana Sofia Coroadinha^1,2, François du Plessis³, Catarina Brito^1,2
1 iBET – Institute of Experimental Biology and Technology
² Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa
³ Boehringer Ingelheim Pharma GmbH & Co. KG
^P Presenting author

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Cardiotoxicity and cardiac efficacy models

P13

Beauvericin-induced cardiotoxicity in H9C2 cardiomyocytes and zebrafish embryos

Ming-Yuan  Liu¹, Jui-Feng  Tsai¹, Biing-Hui Liu^P1
1 Graduate Institute of Toxicology, National Taiwan University
^P Presenting author

P14

Could succination lead to cardiotoxcity

Afaf FIZAZI^P1,2, Cloé  SCHOELING¹, Anne-Emilie DECLEVES², Jean-Marie COLET^1
1 Human biology and toxicology laboratory. University of Mons. Belgium
² Metabolic and Molecular Biochemistry Laboratory. University of Mons. Belgium
^P Presenting author

P15

Action potential waveform analysis in human iPSC-cardiomyocytes enables mechanistic assessment of multichannel cardiac effects

Charlotte Hill^P1, Robert Kirby^1
1 Metrion Biosciences
^P Presenting author

P16

Development and characterization of a high-throughput in vitro human heart model: implications for future pre-clinical cardiac safety screening

Annina Roelofsen^1,2, Nicole Pinckaers^P1,3, Matthijs Blankesteijn^1,2, Misha Vrolijk^1,3, Martijn Hoes^2,4,5
1 Department of Pharmacology and Toxicology, Maastricht University, The Netherlands
² Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands
³ Research Institute of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands
⁴ Department of Clinical Genetics, Maastricht University, Maastricht, Netherlands
⁵ Department of Cardiology, Maastricht University Medical Center, Maastricht, Netherlands
^P Presenting author

Go back

Case studies for successful use and implementation of complex in vitro models

P17

Advanced integrated human in vitro approach to explore gut-brain barrier interactions with gut microbiota metabolites and pesticides

Juliette Poiriez^P1, Maria Abou Diwan^1,2, Avénie Huet^2,3, Claire Joly Condette ², Emmanuel  Sevin ¹, Stéphane Delanaud², Halima Ouadid-Ahidouch³, Véronique  Bach², Fabien Gosselet¹, Hafida Khorsi-Cauet², Pietra Candela^1
1 Laboratoire de la Barrière Hémato-Encéphalique (LBHE), UR 2465, Université d’Artois, 62300 Lens, France
² PERITOX—Périnatalité et Risques Toxiques—UMR_I 01 UPJV/INERIS, Centre Universitaire de Recherche en Santé, CURS-UPJV, Université Picardie Jules Verne, CEDEX 1, 80054 Amiens, France
³ Laboratoire de physiologie cellulaire et moléculaire (LPCM), UR UPJV 4667, Université Picardie Jules Verne, UPJV, 80039 Amiens, France
^P Presenting author

P18

Use of advanced NAMs for food-related nanotoxicology: a case study to investigate synthetic amorphous silica (SAS) toxicity in a liver organoid model

Pierre-Jean Ferron^P1, Anne-Louise Blier¹, Noa Oluyn², Eveline Verleysen², Jan Mast², Andrea Haase³, Francesco Cubadda⁴, Kevin Hogeveen¹, Valérie Fessard^1
1 French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Fougères, FRANCE
² Sciensano, Groeselenbergstraat 99, 1180 Uccle, Belgium
³ German Federal Institute for Risk Assessment (BfR), Berlin, Germany
⁴ Istituto Superiore di Sanita, National Institute of Health (ISS), 00161 Rome, Italy
^P Presenting author

P19

A 3D co-culture model simulating the human hepatic microenvironment

Julia Böhme¹, Dajana Lichtenstein^P1, Neele Wewer¹, Linda Böhmert¹, Maxi B. Paul^1
1 German Federal Institute for Risk Assessment
^P Presenting author

P20

Understanding regulatory uptake of NAMs: a multicriteria review of 20 case methods

Felipe Augusto Pinto-Vidal^P1, Bastiaan  van Ravenhorst², Laure  Geoffroy³, Noelia  Domínguez-Morueco⁴, Thomas  Loret³, Walter  Westerink⁵, Sebastian  Hoffmann^6
1 Institute for Evidence-Based Toxicology
² aQuaTox Solutions
³ French National Institute for Industrial Environment and Risks (INERIS),
⁴ Instituto de Salud Carlos III (ISCIII)
⁵ Charles River Laboratories
⁶ Institute for Evidence-Based Toxicology (IEBT),
^P Presenting author

P21

Β-carotene nanocarriers as a tool to sustain hyaluronic acid and counteract skin ageing: A case study

Zinaida Shakel^P1, Salette Reis¹, Maria Cavinato-Nascimento², Sofia Lima^1
1 University of Porto
² Leopold Franzens Universität Innsbruck
^P Presenting author

P22

Blocking complement as a preventative measure for kidney ischemia reperfusion injury: insights from a human kidney-on-a-chip study

Linda Gijzen¹, Laura Tool¹, Eline Haspeslagh², Rumaisha Annida¹, Tina Thakkar^P1, Jolien Delaere², Kevin Budding², Erik Hack², Inge van de Walle^2
1 MIMETAS B.V, Oegstgeest, The Netherlands
² Argenx B.V, Zwijnaarde, Belgium
^P Presenting author

P23

Adapting RNA-seq as an alternative acquisition platform for gene expression quantification in the GARDskin assay

Andy Forreryd^P1, Tim Lindberg¹, Robin Gradin¹, Henrik Johansson^1
1 SenzaGen AB
^P Presenting author

P24

Addressing the role of maternal inflammation in autism spectrum disorder using immune-competent iPSC derived brain microphysiological systems

Alex Rittenhouse^P1, Jingyi Zhang¹, Jason Laird¹, Lena Smirnova^1
1 Johns Hopkins Bloomberg School of Public Health
^P Presenting author

P25

Novel 3D-printed cell culture inserts for advanced in vitro skin regeneration

Magdalena Metzger^P1, Magdalena Bauer¹, Marvin Corea¹, Barbara Schädl¹, Johannes Grillari¹, Peter Dungel^1
1 Ludwig Boltzmann Institute for Traumatology
^P Presenting author

P26

Continuous in vitro cultivation of a defined human gut microbiota: assessing media-driven shifts using the peristaltic mixed tubular bioreactor (PETR)

Anwar Walid^P1,2, Katrin Dohnt^1,2
1 Institute of biochemical engineering, TU Braunschweig, Germany
² Braunschweig Integrated Centre of Systems Biology (BRICS), TU Braunschweig, Germany
^P Presenting author

P27

Development and application of an immunocompetent in vitro stem cell-based intestinal model for hazard assessment; testing the effects of faba bean, cricket and yellow chlorella proteins

Loes Duivenvoorde¹, Yara  Awad², Benedetta Fabrizi¹, Stéphane Hazebrouck², Aafke Janssen¹, Mustafa Usta¹, Meike van der Zande^P1
1 Wageningen Food Safety Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands
² Département Médicaments et Technologies pour la Santé (DMTS), Université Paris-Saclay, CEA, INRAe, Service de Pharmacologie et d’Immunoanalyse, 91190 Gif-sur-Yvette, France
^P Presenting author

P28

A targeted in vitro approach to fill hazard data gaps in the context of safe and sustainable by design (SSBD): a flame retardants case study

Nienke Ruijter^P1, Mario  Wenger², David Beal¹, Margaux Papin¹, Joséphine Steck³, Martin Himly², Marie Carrière^1
1 Univ. Grenoble-Alpes, CEA, CNRS, Grenoble INP, IRIG, SyMMES, CIBEST, 38000 Grenoble, France
² Department of Biosciences and Medical Biology, Paris Lodron University of Salzburg, Salzburg, 5020, Austria
³ Univ. Grenoble Alpes, CEA, Liten, DTNM, 38000 Grenoble, France
^P Presenting author

P29

Epivaginal™ in Europe: cross-site reproducibility and discriminatory performance using ET-50 and multi-endpoint readouts

Emily Reis^P1, Jam Markus¹, Karol  Forro², Mitchell  Klausner³, Timothy  Landry ³, Kaitlyn  Coen ³, Silvia  Letasiova ¹, Kristina  Kejlová ⁴, Markéta  Dvořáková ⁴, Eliška  Pácalová ^4
1 MatTek IVLSL, Bratislava, Slovakia
² Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Bratislava, Slovakia
³ MatTek Corporation, Ashland, MA, USA
⁴ Centre of Toxicology and Health Safety, National Institute of Public Health, Prague, Czech Republic
^P Presenting author

P30

Enhanced oxygen supply enables sustained function of primary human hepatocyte spheroids

YOSUKE MATSUMOTO^P1, YUICHI SHIRASAKI¹, KOJI NAKAZAWA^2
1 Taica Corporation
² University of Kitakyushu
^P Presenting author

Go back

Challenges in cosmetics safety  

P31

Safety assessment of botanicals and natural complex substances

Ashwin Shah^P1, Iris Müller¹, Paul Benndorf¹, Carmon Kee¹, Özge Osmanoglu¹, Nadia Heramvand¹, Simone Hoffmann-Dörr^1
1 Henkel AG & Co. KGaA
^P Presenting author

P32

Reciprocal interaction between PPARγ and CEBPα in lipid metabolism regulation: hypothesis of a self-sustaining regulatory loop in HaCaT cells

Lorenzo Dondero^P1,2, Giulia De Negri Atanasio^1,3, Francesca  Tardanico^1,3, Matteo Sergiampietri¹, Erica Lertora^1,3,4, Bruno Burlando⁵, Ilaria  Demori^5
1 Department of Earth, Environmental, and Life Sciences (DISTAV), University of Genoa, 16132 Genoa, Italy
²  Interuniversity Center for the Promotion of 3R Principles in Teaching and Research (Centro 3R), Italy
³ Interuniversity Center for the Promotion of 3R Principles in Teaching and Research (Centro 3R), Italy
⁴ Angel Consulting S.a.s

P33

Development of a senior canine skin model for NAM-based safety assessment and efficacy of novel pet cosmetic ingredients

Minji Kim^P1, Heeyeon  Cho¹, Eun-jin Park¹, Kyung-Eun Lee¹, Chunho Park^1
1 COSMAXBTI
^P Presenting author

P34

Permeability of hazardous agents in cooling lubricants after application of occupational skin protection products

Vanessa Geyik^P1, Magdalena Metzger¹, Anna Szorger¹, Clemens Dobusch², Parisa Ansari², Peter Dungel^1
1 Ludwig Boltzmann Institute for Traumatology
² Austrian Workers’ Compensation Board AUVA
^P Presenting author

P35

Addressing toxicological data gaps in cosmetic safety assessment: A case study on the regulatory challenges related to the use of read across and NAMs

Sanghamitra Mishra^P1, Avneet Kaur Suri², Celine  De Roy¹, Ankushreddy Patil², Monica Autiero¹, Thomas Petry^1
1 ToxMinds BV
² ToxMinds India Consulting Pvt. Ltd.
^P Presenting author

P36

Optical coherence tomography enhances mecanistic evaluation of ocular irritation in an ex vivo bovine eye model

Wiam NEDJAR^P1, Soumia CHAOUCHE¹, Chaîma TOUILEB¹, Imadeddine ZEBBICHE², Younes ZEBBICHE^1
1 Department of Industrial Pharmacy – Faculty of Pharmacy – University of Health Sciences, Algiers
² FOCUS CENTER
^P Presenting author

Go back

Computational toxicology – in silico modelling, read-across, artificial intelligence and machine learning

P37

Development of an in silico approach for predicting human internal exposure to topically applied cosmetic ingredients in NGRA

Shimpei Terasaka^P1, Kazutoshi Saitou¹, Yuuichi Itou^1
1 Safety Science Research Laboratory, Kao Corporation, Japan
^P Presenting author

P38

Computational prediction of the kinetic and toxicological parameters of cylindrospermopsin

Antonio Cascajosa-Lira¹, Remedios Guzmán-Guillén ^P1, Silvia Pichardo¹, Ana María Cameán¹, Angeles Jos¹, Ana I. Prieto^1
1 Toxicology Area, Faculty of Pharmacy, University of Seville, Spain.
^P Presenting author

P39

Preliminary machine learning approach to the immunotoxicity of cosmetic related PFAS

Lici Damar Cristina Diaz Bambague^P1, William Giovanni Quevedo Buitrago¹, Luis Fernando Nino Vasquez^1
1 Universidad Nacional de Colombia
^P Presenting author

P40

Evolution of AI-based toxicity prediction and the emerging role of third-generation architectures

Kohtaro Yuta^P1
1 In Silico Data, Ltd.
^P Presenting author

P41

EndocrineAI: an AI-driven platform for endocrine disruption screening

Bharath BR^P1, Jahnavi S¹, Nadeem Khan^1
1 Jai Research Foundation
^P Presenting author

P42

Establishment of readiness criteria for AI-models for risk assessment: ReadyAI

Joyce de Paula Souza^P1,2, Jonathan  Blum^1,2, Uko  Maran ³, Sulev  Sild ³, Louis Dawson⁴, Laura  Holden ⁴, Robert  Lee ⁴, Aleksandra  Čavoški ⁴, Veronika  Karnel ⁵, Lukas Meusburger⁵, Sandrine  Fraize-Frontier⁶, Alexander Walsh⁶, Gilles Rivière⁶, Giuseppa Raitano⁷, Alessandra  Roncaglioni ⁷, Emma   Di Consiglio ⁸, Olga  Tcheremenskaia ⁸, Cecilia Bossa⁸, Lina  Wendt-Rasch⁹, Michal Kalapus¹⁰, Tomasz  Puzyn ¹⁰, Ellen  Fritsche ^1,2
1 Swiss Centre for Applied Human Toxicology, Switzerland
² Department of Pharmaceutical Sciences, University of Basel, Switzerland
³ Institute of Chemistry, University of Tartu, Estonia
⁴ Birmingham Law School, University of Birmingham, United Kingdom
⁵ Austrian Agency for Health and Food Safety (AGES), Department Risk Assessment, Austria
⁶ French Agency for Food, Environmental and Occupational Health & Safety (ANSES), France
⁷ Department of Environmental Health Sciences, Mario Negri Institute for Pharmacological Research, Italy
⁸ Environment & Health Department, Italian National Institute of Health (ISS), Italy
⁹ Swedish Chemicals Agency (KemI), Sweden
¹⁰ Faculty of Chemistry, University of Gdansk, Poland
^P Presenting author

P43

Cheminformatics interaction profiles implicate the UV filter benzophenone-4 as a potential obesogen via PPARY activation

Junpyo Gong^P1, Minsoo Noh^1
1 College of Pharmacy & Natural Products Research Institute, Seoul National University, Seoul, Republic of Korea
^P Presenting author

P44

Building a quantitative adverse outcome pathway for AOP #38 – protein alkylation leads to liver fibrosis

Levi Winkelman^P1, Joost Beltman¹, James Stevens^1
1 Division of Cell-systems and Drug Safety, LACDR, Leiden University, the Netherlands
^P Presenting author

P45

Uncovering compound mode of action via hierarchical clustering of structural and morphological profiles

Floriane Odje^P1, Elena  von Coburg², Sebastian Dunst², Andrea Volkamer^1
1 Universität des Saarlandes
² German Centre for the Protection of Laboratory Animals (Bf3R)
^P Presenting author

P46

Enhancing in silico prediction of Ames mutagenicity for organosilicon compounds and exploring chemical space boundaries

Barbara G.  Schmitt^P1, Emilio Benfenati², Robert S.  Foster³, Grace Kocks³, Guiseppa Raitano², Rachael E.  Tennant³, Carole Forlini⁴, Eckart Gura⁵, Shawn Seidel⁶, Farah Koraichi-Emeriau^7
1 Evonik Operations GmbH
² Istituto di Ricerche Farmacologiche Mario Negri
³ Lhasa Ltd.
⁴ Elkem Silicones France SAS
⁵ Wacker Chemie AG
⁶ The Dow Chemical Company
⁷ Equitox
^P Presenting author

P47

Systematic linkage of transcriptomics data to adverse outcome pathways for toxicity prediction

Sidra Adil^P1, Theo de Kok¹, Egon Willighagen¹, Tooba Abbassi-Daloii¹, Marvin Martens^1
1 Department of Translational Genomics (TGX), NUTRIM, Maastricht University, Netherlands
^P Presenting author

P48

AI4AOPOMICS: artificial intelligence for integrating omics data into adverse outcome pathways

Donghyeon Kim^P1,2, Théo Jamay², Florence Jornod², Jinhee Choi¹, Karine Audouze^2
1 School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, Dongdaemun-gu, Seoul 02504, Republic of Korea
² Université Paris-Cité, Inserm, HealthFex, SysTox team, 45 rue des Saints-Pères, 75006 Paris, France
^P Presenting author

P49

Validation of AI-based toxicity prediction models with reference chemicals for regulatory applications

Donghyeon Kim^P1, Siyeol Ahn¹, Jiyong Jeong¹, Jinhee Choi^1
1 School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, Dongdaemun-gu, Seoul 02504, Republic of Korea
^P Presenting author

P50

Decoding biological processes through multi-omic network diffusion

Antoine Bodein¹, Marie-Pier Scott-Boyer¹, Kahina Abed², Gladys Ouedraogo², Romain Grall^P2, Anne Riu², Olivier Perin², Arnaud Droit^1
1 1Molecular Medicine Department, CHU de Québec Research Center, Université Laval, Québec, QC, Canada
² L’Oréal Research & Innovation, Aulnay-sous-Bois, France 
^P Presenting author

P51

Chemical clustering of natural complex substance (NCS) materials: A hierarchical clustering approach for use in non-animal safety assessments

Holger Moustakas^P1, Jake Muldoon¹, Trevor Penning², Amanda Bryant-Friedrich³, Mark Cronin⁴, Daniel Liebler⁵, Danielle Botelho¹, Anne Marie Api^1
1 Research Institute of Fragrance Materials, Inc., 1200 MacArthur Boulevard, Mahwah, NJ 07430, USA
² Center of Excellence in Environmental Toxicology, The University of Pennsylvania, Perelman School of Medicine, 1315 Biomedical Research Building (BRB) II/III, 421 Curie Boulevard, Philadelphia, PA, 19104-3083, USA
³ Wayne State University Graduate School Professor of Pharmaceutical Sciences, 5057 Woodward Avenue, Suite 11208, Detroit, MI, 48202, USA
⁴ School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF, UK
⁵ Department of Biochemistry, Vanderbilt University, B3301A Medical Center North 465 21st Avenue South, Nashville, TN, 37232-6350, USA
^P Presenting author

P52

Advancing chemical grouping: development and application of signature-based structure-activity groups for non-animal safety assessments

Jake Muldoon¹, Holger Moustakas^P1, Terry Schultz², Trevor Penning³, Amanda Bryant-Friedrich⁴, Danielle Botelho¹, Anne Marie Api^1
1 Research Institute of Fragrance Materials, Inc., 1200 MacArthur Boulevard, Mahwah, NJ 07430, USA
² The University of Tennessee, College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996-4500, USA
³ Center of Excellence in Environmental Toxicology, The University of Pennsylvania, Perelman School of Medicine, 1315 Biomedical Research Building (BRB) II/III, 421 Curie Boulevard, Philadelphia, PA, 19104-3083, USA
⁴ Wayne State University Graduate School Professor of Pharmaceutical Sciences, 5057 Woodward Avenue, Suite 11208, Detroit, MI, 48202, USA
^P Presenting author

P53

An approach to characterise and define tolerable uncertainty in read-across for toxicity prediction

Mark Cronin^P1, Terry Schultz^2
1 Liverpool John Moores University, Liverpool, UK
² University of Tennessee, Knoxville TN, USA
^P Presenting author

P54

Heme metabolism and mycotoxins: lessons learned from Atlantic salmon – a computational multi-target investigation

Lorenzo Pedroni¹, Ole J. Nøstbakken ², Kai K. Lie², Chiara Dall’Asta¹, Luca Dellafiora^P1
1 Department of Food and Drug, University of Parma, Parma, Italy
² Institute of Marine Research, Bergen, Norway
^P Presenting author

P55

Guardrailed, auditable agentic workflows for computational toxicology via the model context protocol (MCP)

Ivo Djidrovski^P1
1 Institute for Risk Assessment Sciences (IRAS), Utrecht University
^P Presenting author

P56

O-QT: auditable AI-assisted QSAR toolbox read-across workflows for VHP4Safety implementation

Ivo Djidrovski^P1, Juliette Legler¹, Raymond Pieters^1,2, Marc Teunis^2
1 Institute for Risk Assessment Sciences (IRAS), Utrecht University
² HU-University of Applied Sciences, Innovative Testing, Utrecht, the Netherlands
^P Presenting author

P57

Macrophage-explicit PBPK model predicts long-term human organ accumulation of ingested microplastics

Shohei Kaneko^P1, Gaku Shimodaira¹, Takeshi Katsuda¹, Masaki Nishikawa¹, Yasuyuki Sakai^1
1 Dept. of Chemical System Engineering, School of Engineering, The University of Tokyo
^P Presenting author

P58

Automated quantification of collagen gel contraction using a fine-tuned foundation model for in vitro toxicity assessment

Sandeul Ryu^P1, Minju Kim², Heesung Hwang¹, Yejin Park¹, Jiwon Yoo¹, Juhee Lee^3,1, Seung Min Oh^2,1,4
1 Department of Bio-application Toxicity, Hoseo University, Asan, Republic of Korea.
² Animal Welfare & Alternative Testing Research Center, Hoseo University, Asan, Republic of Korea.
³ Department of ICT Automotive Engineering, Hoseo University, Asan, Republic of Korea.
⁴ Department of Animal Health and Welfare, Hoseo University, Asan, Republic of Korea.
^P Presenting author

P59

Case study on the use of AOP-based AI model for prioritization of endocrine disrupting chemicals from consumer products

Donghyeon Kim^P1,2, Jaeseong Jeong¹, Ajaya Kumar Sahoo¹, Sangha Ahn¹, Gaeul Yang¹, Chaein Chong¹, Jinhee Choi^1
1 School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, Dongdaemun-gu, Seoul 02504, Republic of Korea
² Université Paris-Cité, Inserm, HealthFex, SysTox team, 45 rue des Saints-Pères, 75006 Paris, France
^P Presenting author

P60

A hybrid in silico/in vitro approach to address applicability-domain limitations in chemical carcinogenicity prediction

Ani Tevosyan^P1, Hayk Daghunts¹, Lusine Khondkaryan¹, Gohar Tadevosyan¹, Lilit Apresyan¹, Anna Misakyan¹, Zaven Navoyan², Nelly Babayan^1,2
1 Institute of Molecular Biology, NAS RA
² Toxometris.ai Inc.
^P Presenting author

P61

Co-regulated gene network analysis to uncover mechanisms of liver toxicity liabilities in vitro

Sibel Bahtiri^P1, Steven Kunnen¹, Lukas Wijaya¹, Tessa Hagens¹, Giulia Callegaro¹, Marije Niemeijer¹, Bob van de Water^1
1 Leiden Academic Centre for Drug Research
^P Presenting author

P62

Using AI tools for systematic literature search, appraisal, and data extraction for the development of adverse outcome pathways

Roseline Awoga^P1, Vajagathali Mohammed¹, Nefeli C Vytinioti^1,2, Anna Forsby^1
1 Stockholm University
² Karolinska Institute
^P Presenting author

P63

Integrated analysis of cellular pathways of respiratory nanotoxicity

Jiating Deng^P1, Lianyong Han¹, Carola  Voss², Tobias  Stöger^1
1 Institute of Lung Health and Immunity (LHI), Helmholtz Center Munich
² Hannover Medical School, Clinic for Cardiac, Thoracic, Transplantation and Vascular Surgery, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO); Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German  Center for Lung Research (DZL), Hannover, Germany
^P Presenting author

Go back

ECHA, EMA and EFSA perspectives on the use of NAMs

P64

EFSA’s proposed assessment strategy for waiving of dog studies in pesticide approval

Adriana Scattareggia Marchese^P1, Martina Panzarea¹, Judy Choi², Andrea Terron¹, Jochem Louisse¹, Tamara Coja³, Antonio Hernàndez-Jerez⁴, Olavi Pelkonen^5
1 European Food Safety Authority (EFSA)
² German Federal Institute for Risk Assessment (BfR)
³ Austrian Agency for Health and Food Safety (AGES)
⁴ University of Granada
⁵ University of Oulu
^P Presenting author

P65

Integration of new approach methodologies in the toxicological and allergenicity assessment of novel foods: EFSA’s tiered approach

Maria Glymenaki^P1,2, Marcello  Laganaro¹, Irene  Nuin Garciarena¹, Annamaria  Rossi¹, Maura  Magani¹, Ermolaos  Ververis¹, Andrea  Germini¹, Océane  Albert^1
1 European Food Safety Authority, Nutrition and Food Innovation Unit, Parma, Italy
² Department of Metabolism Digestion and Reproduction, Imperial College London, United Kingdom
^P Presenting author

P66

Integrating new approach methodologies in the risk assessment of decontaminating substances for foods of animal origin

CRISTINA CROERA^P1, BLANKA HALAMODA^1
1 European Food Safety Authority (EFSA), Food Ingredients and Packaging (FIP) Unit, Parma, Italy
^P Presenting author

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Funding the development, validation and implementation of NAMs

P67

What have we achieved? A retrospective assessment of 3Rs projects funded by the set foundation

Katja Sommer¹, Claus-Michael Lehr^1,2, Kristina Wagner^P1,3, Joachim Coenen^1,4
1 Foundation for the Promotion of Alternatives to Animal Testing (set Foundation), Frankfurt am Main, Germany
² Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Saarbrücken, Germany
³ German Animal Welfare Federation – Animal Welfare Academy, Neubiberg, Germany
⁴ Merck KGaA, Darmstadt, Germany
^P Presenting author

P68

Human residual tissue becomes a valuable source in scientific research

Gerry Wagenaar^P1, Ilse Duivenvoorden¹, Sjors van Kats^1
1 Department of VitalTissue, ETB-BISLIFE, Jan van Krimpenweg 17, 2031 CG Haarlem, The Netherlands
^P Presenting author

P69

Landscape of challenges for in vitro testing of complex mixtures.

Benjamin KOPP^P1, Marion Alriquet², Maricel Marin-Kuan², Marie-Christine Chagnon^3
1 Gnosis by Lesaffre, Marcq-en-Baroeul, France
² Société des Produits Nestlé S.A., Nestlé Research, Lausanne, Switzerland
³ Institut Agro Dijon, France
^P Presenting author

P70

Comparative RNA-seq profiling of 2D, static 3D and dynamic 3D cultures and their concordance with human tissue expression

LAURA GOMEZ CUADRADO^P1, DAVID PALACIOS², MARTA SENDRA^1,2, DALIA DE LA FUENTE-VIVAS¹, ROCÍO BARROS¹, SONIA MARTEL-MARTÍN¹, PILAR MUÑIZ^2
1 International Research Center in Critical Raw Materials for Advanced Industrial Technologies (ICCRAM), R&D Center, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001, Burgos, Spain
² Department of Biotechnology and Food Science, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos, 09001, Burgos, Spain
^P Presenting author

In vitro and in silico methods for safety assessment of medical devices

P71

In vitro sensitization assessment of medical device extracts: prevalidation results of GARDskin medical device for potential inclusion in ISO 10993-10

Andy Forreryd^P1, Per Alftrén¹, Robin Gradin¹, Henrik Johansson¹, Tim Lindberg^1
1 SenzaGen AB
^P Presenting author

P73

Replace animal tests (RAT) list – medical devices

Emma Grange^P1, Kate Shipton¹, Laura Alvarez^1
1 Cruelty Free International
^P Presenting author

In vitro systems to assess respiratory toxicity

P74

A comprehensive model for predicting the toxicology of mineral fibres by connecting the fibre parameters to the key characteristics of carcinogens

Alessandro Gualtieri¹, Serena Mirata^2,3, Vanessa Almonti⁴, Mario Passalacqua^5,3, Stefania Vernazza^5,3, Anna Maria Bassi^5,3, Armanda Pugnaloni⁶, Sonia Scarfì^P2,3
1 Dept. of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Modena, Italy
² Dept. Earth, Environment and Life Sciences, University of Genova, Itay
³ Inter-University Centre for the Promotion of the 3Rs Principles in Teaching & Research, Turin, Italy
⁴ Center for Advanced Technologies, Poznan, Poland
⁵ Dept. Experimental Medicine, University of Genova, Italy
⁶ Dept. Clinical and Molecular Sciences, Polytechnic University of Marche, Torrette di Ancona, Italy
^P Presenting author

P75

Acellular in vitro dissolution of man-made vitreous fibers in synthetic lung fluids using single fiber and USP-4 (united states pharmacopeia apparatus 4) setups

Denis V. Okhrimenko^P1, Saetlai Z. Khai^2,1, Alexandra Beltrami³, Pia Nielsen¹, Susan L.S. Stipp³, Yuanzheng Yue², Mette Solvang^1
1 ROCKWOOL A/S
² Aalborg University
³ Technical University of Denmark
^P Presenting author

P76

Cytotoxicity assessment of solid fuel particulate matter using a “cells-on-particles” platform

Gailė Pocevičiūtė^P1,  Violeta Kaunelienė¹, Edvardas Bagdonas², Jurgita Ovadnevaitė³, Darius Čiužas¹, Dainius Martuzevičius^1
1 Department of Environmental Technology, Faculty of Chemical Technology, Kaunas University of Technology, Lithuania
² Department of Regenerative Medicine, Centre for Innovative Medicine, Vilnius, Lithuania
³ School of Natural Sciences, Physics, University of Galway, Galway, Ireland
^P Presenting author

P77

Assessing the toxicity of traffic-derived air pollution using a primary human air-liquid interface airway in vitro model

Gerrit Bredeck^1,2, Tina Wahle¹, Angela Kämpfer¹, Jochen Dobner¹, John Boere³, Paul Fokkens³, Evert Duistermaat³, Tim Spannbrucker¹, Andrea Rossi¹, Flemming Cassee^3,4, Roel Schins^1,5
1 IUF – Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany
² Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), Madrid, Spain
³ Centre for Sustainability, Environment and Health, National Institute for Public Health and the Environment, Bilthoven, the Netherlands
⁴ Institute for Risk Assessment Sciences (IRAS), Utrecht University, Utrecht, the Netherlands
⁵ Department of Pharmacology and Toxicology, Maastricht University, Maastricht, the Netherlands
^P Presenting author

P78

Respiratory uptake of inhaled dinoseb and rotenone using primary bronchial epithelial cells

Yvonne Staal^P1, Véronique de Bruijn¹, Jolanda Vermeulen¹, Theo Sinnige², Edwin Zwart¹, Eline Groeneveld¹, Anne Kienhuis^1,2
1 RIVM
² Utrecht University
^P Presenting author

P79

Advancing non-animal respiratory toxicology: a TRPA1-based in vitro model for airway irritant detection

Robert Bedford^P1
1 Labcorp Early Development Laboratories Ltd.
^P Presenting author

P80

Assessing inhalation toxicity of cosmetic compounds on upper airway and alveolar air-liquid interface models after liquid or nebulized exposure

Carolin Göbel^P1, Gerlinde Pappa¹, Andreas  Schepky¹, Daniela Lange^1
1 Beiersdorf AG, Hamburg, Germany
^P Presenting author

P81

Breathing small airways lung-on-chip to model COPD pathophysiology and assess lung toxicity

Marco Mondini^P1, Valeria Fragozo Mesa², Gessica Marchini³, Roberta Visone^1
1 BiomimX Srl
² Politecnico di Milano
³ Chiesi Farmaceutici Spa
^P Presenting author

P82

In silico evaluation of inhalation exposure to essential oils

Adeline NALIN¹, Manon FOURNIER^P1, Sébastien GREGOIRE^2
1 AROMA-ZONE
² Sébastien Grégoire Consulting
^P Presenting author

P83

Towards prospective identification of respiratory sensitizers: setting up a co-culture of bronchial epithelial and dendritic-like cells

Eline Groeneveld¹, Evert Duistermaat², Renée de Boeck², Jolanda Vermeulen¹, Eric Gremmer¹, Rob vandebriel¹, Véronique de Bruijn^P1
1 Centre for Health Protection, National Institute of Public Health & the Environment
² Centre for Sustainability, Environment and Health, National Institute of Public Health & the Environment
^P Presenting author

P84

The exposure to inhalable substances and their impact on the safety assessment of cosmetic products and their ingredients

Erica Lertora^P1,2,3, Giulia  De Negri Atanasio^1,3, Lorenzo Dondero^1,3, Francesca Tardanico^1,3, Matteo  Sergiampietri¹, Serena  Mirata¹, Matteo  Zanotti Russo², Sonia  Scarfì^1,3, Elena Grasselli^1,3,4
1 Department of Earth, Environment and Life Science, University of Genoa, Corso Europa 26, Genoa, Italy
² Angel Consulting, Via San Senatore 14, 20122, Milan, Italy
³ Interuniversity Center for the Promotion of 3R Principles in Teaching and Research (Centro 3R), Italy
⁴ National Center for the Development of New Technologies in Agriculture (Agritech), 80121, Naples, Italy
^P Presenting author

P85

Effects of ozone on lung cells exposed at the air-liquid interface under controlled conditions.

Louise VERHAEGHE^P1,2, Suen BOULE^1,2, Dominique COURCOT^1,2, Yann LANDKOCZ^1,2
1 UCEiV UR4492 – Dunkerque – France
² Université du Littoral Côte d’Opale – Dunkerque – France
^P Presenting author

P86

Phthalates alone or in a mixture induce mitochondrial dysfunction and phenotypic modification in bronchial cells

Lyse Szymanski^P1, Margueritta  Al Zallouha¹, Clémence Bouzar¹, Edwige  Votier¹, Christelle  Monteil ¹, Tiphaine Rogez-Florent ^1
1 Univ Rouen Normandie, Université Caen Normandie, Normandie Univ, ABTE UR 4651, F-76000 Rouen
^P Presenting author

P87

Mixture toxicity of benzene, toluene, ethylbenzene, and xylene (BTEX) in a human organotypic model of the respiratory tract exposed acutely or repeatedly at the air/liquid interface

Hugo Bes^P1, Fabrice Bray², Nour Jaber¹, Dorothée Dewaële³, Stéphanie Devassine², Christian Rolando², Fabrice Cazier³, Claude Emond⁴, Sylvain Billet^1
1 UR4492 Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral Côte d’Opale
² Univ. Lille, CNRS, USR 3290 – MSAP
³ CCM, Centre Commun de Mesures, Université du Littoral Côte d’Opale
⁴ PKSH Inc, Crabtree, Québec, Canada
^P Presenting author

P88

Study of the toxicity of prenol, a second generation biofuel, by coupling an atmospheric simulation chamber to an air-liquid interface exposure device

Reem Al Mawla^1,2, Cécile Coeur², Nicolas Houzel², Sylvain Billet^P1
1 UR4492 Unité de chimie environnementale et interactions sur le vivant (UCEIV), Université du Littoral Côte d’Opale, Dunkerque, France
² UR4493 Laboratoire de Physico-Chimie de l’Atmosphère (LPCA), Université Littoral Côte d’Opale, Dunkerque, France
^P Presenting author

P89

In vitro immune off-target toxicity assessment using morph_ONE for antibody drug conjugates (ADCs)

Ewelina Hoffman^P1, Josephine Joel¹, Elinda Zequiri¹, Rhamiya Mahendran¹, Victoria Hutter^1,2
1 ImmuONE Limited, Sycamore House, Stevenage, Herts, UK, SG1 2BP
² Centre for Topical Drug Delivery and Toxicology, University of Hertfordshire, Hatfield, Herts, UK, AL10 9AB
^P Presenting author

P90

An advanced airway-on-a-chip model with unidirectional flow and perfusable microvascular network for studying fibrosis

Artur  Rodrigues¹, Iris Schilt¹, Alexis  Dalaud¹, Camilla Clavijo¹, Tina hakkar^P1, Job Komen¹, Todd  Burton¹, Lenie van den Broek^1
1 MIMETAS B.V, Oegstgeest, The Netherlands
^P Presenting author

P91

A novel adverse outcome pathway for respiratory sensitisation: challenges and opportunities for in vitro safety assessment using new approach methodologies (NAMs)

Markéta Dvořáková^P1
1 National Institute of Public Health
^P Presenting author

P92

Establishment of a human pulmonary in vitro co-culture model for nanoplastic toxicological assessment: calu-3 and THP-1 macrophages

Claudia Anguita Solé¹, Javier Gutiérrez García¹, Alba García-Rodríguez¹, Laura Rubio Lorente^P1, Alba Hernández Bonilla^1
1 Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain
^P Presenting author

P94

Cooling agents in e-cigarettes: toxicological evaluation of WS-3, WS-5 and WS-23 in bronchial epithelial models

Aleksandra Khomutetckaia^P1
1 German Federal Institute for Risk Assessment
^P Presenting author

P95

Transcriptomic and cytokine responses of reconstructed human bronchial epithelium to respiratory versus skin sensitizers

Honoka Aoshima^P1, Kanae Ishimori¹, Shinkichi Ishikawa¹, Izuru Mizoguchi², Takayuki Yoshimoto², Takao Ashikaga^3
1 Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., Kanagawa, Japan
² Department of Immunoregulation, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan
³ Division of Genome Safety Science, National Institute of Health Sciences Center for Biological Safety and Research, Kanagawa, Japan
^P Presenting author

P96

The USP-4 as a tool to assess the dissolution of man-made vitreous fibres

Léa Hiéronimus^P1, Julien de Cruz¹, Denis Okrimenko², John Hoffman³, Heather Kessler³, Dan Edson³, Maxime Jacquemin⁴, Quentin Hérault⁴, Natasa Drnovsek⁵, Urška  Kovačič ⁵, Jordi Aixart⁶, Nerea Mascaraque⁶, Keith Rickabaugh⁷, Brandon Wiggins⁷, Amy Madl⁸, Mette Solvang^2
1 EURIMA, Rue de Deux Eglises 29, 1000 Brussels, Belgium
² ROCKWOOL A/S, Hovedgaden 584, 2640 Hedehusene, Denmark
³ Owens Corning S&T, 2790 Columbus Road, Granville, OH 43023, USA
⁴ Saint-Gobain Research Paris, 41 quai Lucien Lefranc, 93300 Aubervilliers, France
⁵ Knauf Insulation, Rue de Maestricht 95, 4600 Vise, Belgium
⁶ URSA Insulation S.A., Paseo de Recoletos 3, Madrid 28004, Spain
⁷ RJ Lee Group, Inc., 800 Presque Isle Drive, Pittsburgh, PA 15239, USA
⁸ Valeo Sciences LLC, 333 Corporate Drive, Suite 130, Ladera Ranch, CA 92694, USA
^P Presenting author

P97

Estimation of respiratory irritation potency of industrial chemicals by using the TRPV1 expressing SH-SY5Y cell model and machine learning

María Hinojosa^1,2, Gunnar Johanson³, Ulf Norinder^4,5, Anna Forsby^P1
1 Department of Biochemistry and Biophysics, Stockholm University, Sweden
² Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden
³ Institute of Environmental Medicine, Karolinska Institutet, Stockholm Sweden
⁴ MTM research Centre, School of Science and Technology, Örebro University, Sweden
⁵ Department of Pharmaceutical Biosciences, Uppsala University, Sweden
^P Presenting author

P98

Single-cell mass cytometry reveals multicellular context–dependent reshaping of oxidative stress–related key events following Ceria nanoparticle exposure

Eunseo Lee^P1, Hyojoo Bang¹, Seung-Geun Park¹, Tae Hyun Yoon^1
1 Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea
^P Presenting author

P99

An AOP-based in vitro evaluation of humidifier disinfectant–induced pulmonary fibrosis using a 3D human epithelial–fibroblast co-culture model

Minju Kim^P1, Heesung Hwang², Yejin Park², Sandeul  Ryu², Jiwon Yoo², Juhee Lee^2,3, Seung Min Oh^1,2,4
1 Animal Welfare & Alternative Testing Research Center, Hoseo University, Asan, Republic of Korea
² Department of Bio-application Toxicity, Hoseo University, Asan, Republic of Korea
³ Department of ICT Automotive Engineering, Hoseo University, Asan, Republic of Korea
⁴ Department of Animal Health and Welfare, Hoseo University, Asan, Republic of Korea
^P Presenting author

P100

Short-term exposure to titanium dioxide nanoparticles induces transformation-associated phenotypes in human bronchial epithelial cells

Yejin Park^P1, Ei Ju Heo¹, Minju Kim², Heesung Hwang¹, Sandeul Ryu¹, Jiwon Yoo¹, Juhee Lee^1,3, Seung Min Oh^2,1,4
1 Department of Bio-application Toxicity, Hoseo University, Asan, Republic of Korea.
² Animal Welfare & Alternative Testing Research Center, Hoseo University, Asan, Republic of Korea.
³ Department of ICT Automotive Engineering, Hoseo University, Asan, Republic of Korea.
⁴ Department of Animal Health and Welfare, Hoseo University, Asan, Republic of Korea.
^P Presenting author

P101

From primary NHBE cells to functional 3D inverted bronchial tissue: high-content imaging‑based monitoring of differentiation

Eline Berghmans¹, An Jacobs¹, Inge Nelissen¹, Sandra Verstraelen^1
1 Flemish Institute for Technological Research (VITO), Environmental Intelligence (EI) Unit, Mol, Belgium
^P Presenting author

P102

Applicability of the soluairway™ human airway model as a NAMs platform for in vitro acute respiratory toxicity testing

Ji-Woo  Choe ^1,2, Eun-Ji Gwak^P3, Geon-Hee  Lee³, Su-Hyun Lee³, Ga-Eun Kim⁴, Ha-Ryong Kim⁴, Kyung-Min  Lim ^1,2
1 College of Pharmacy and Graduate School of Pharmaceurical Sciences, Ewha Womans University, Seoul 03760, Republic of Korea
² Graduated Program in Innovative Biomaterials Convergence, Ewha Womans University, Seoul 03760, Republic of Korea
³ R&D Institute, Biosolution Co., Ltd., Seoul, Republic of Korea
⁴ College of Pharmacy, Korea University, Sejong 30019, Republic of Korea
^P Presenting author

P103

A human systemic model for simultaneously evaluating antibody-drug conjugates efficacy and the risk of interstitial lung disease

Jimmy  Vernaz¹, Xiao-Yann Huang^P1, Samuel  Constant¹, Clelia Bourgoint², Gregory Segala², Aurelien Roux³, Simon Latour^3
1 Epithelix
² FluoSphera SA
³ University of Geneva
^P Presenting author

P104

Impact of berry-flavoured nicotine-free e-liquids on an in vitro model of the alveolar-capillary barrier: in vitro toxicity evaluation and chemical analysis

Emma Filaudeau^P1,2, Amelia-Naomi Sabo^1,3, Anita Lebert¹, Véronique Kemmel^1,3
1 Laboratoire de Pharmacologie et Toxicologie Neurocardiovasculaire – UR7296, Université de Strasbourg, Strasbourg, France
² GAIATREND, Rohrbach-lès-Bitche, FRANCE
³ Laboratoire de Biochimie et Biologie Moléculaire, Hôpitaux Universitaires de Strasbourg, Strasbourg, France
^P Presenting author

P105

Lung surfactant inhibition assay – a method to predict early signs of acute inhalation toxicity

Jorid Sørli^P1
1 The National Research Centre for the Working Environment
^P Presenting author

P106

Mechanistic assessment of methyl methacrylate using human relevant new approach methodologies

Sabina Burla¹, Kai Blumbach², Frank Faulhammer³, Tina M. Sommer², Helena Obernolte⁴, Katherina Sewald⁴, Servane Contal⁵, Sebastien Cambier⁵, Karin Wiench^3
1 INVITROLIZE
² Evonik Operations GmbH
³ BASF SE
⁴ Fraunhofer ITEM
⁵ LIST
^P Presenting author

P107

Searching minimal coculture conditions to reproduce the initial inflammatory response to inhaled carbon nanoparticles

Osama Huzain^P1, Lianyong Han¹, Carola Voss², Tobias Stöger^1
1 Institute of Lung Health and Immunity (LHI), Comprehensive Pneumology Center (CPC), Helmholtz Center Munich, Germany;  German Center for Lung Research (DZL), Munich , Germany
² Hannover Medical School, Clinic for Cardiac, Thoracic, Transplantation and Vascular Surgery, Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover, Germany;  Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany
^P Presenting author

P108

Impact of nicotine on calcium flux and markers of endoplasmic reticulum stress in vitro

Anita LEBERT^P1, Amelia-Naomi SABO^1,2, Emma Filaudeau^1,3, Manon DIFFOR¹, Véronique  KEMMEL^1,2
1 Laboratoire de Pharmacologie et Toxicologie Neurocardiovasculaire – UR7296, Centre de Recherche en Biomédecine, Université de Strasbourg, Strasbourg, France 
² Laboratoire de Biochimie et Biologie Moléculaire, Hôpitaux Universitaires de Strasbourg, Strasbourg, France
³ GAIATREND, Rohrbach-lès-Bitche, France
^P Presenting author

Knowledge sharing and education

P109

The convener: A trade association’s role in advancing animal-free science for chemicals safety assessment

Francesca Rapolla^P1, Caroline Rainsford^1
1 Cosmetic, Toiletry and Perfumery Association
^P Presenting author

P110

Inova hub: illuminating the full landscape of NAMs

Jennifer Venhorst^P1, Pedro Simões Ruas¹, Tanja Rouhani Rankouhi¹, Jochem de Vries¹, Erik Lemcke^1
1 TNO
^P Presenting author

P111

Science communication: bridging the gap between animal research and new approach methodologies (NAMs): a knowledge sharing campaign

Monicah Kirathi¹, Francois Busquet^P1, Leonie  Mueller^1
1 Altertox
^P Presenting author

P112

The potential protection of Tamarix extracts against toxicity induced by selected beauty products in Hs27 human skin fibroblasts.

ABDULLAH  ALNUQAYDAN^P1, Faten M.  Ali Zainy,^2
1 Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University
² Chemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia
^P Presenting author

P113

Re-place: a national platform to strengthen dissemination and uptake of new approach methodologies in Belgium

Maude Everaert¹, Mieke Van Mulders², Vera Rogiers¹, Birgit Mertens^P2
1 In Vitro Toxicology and Dermato-cosmetology, Vrije Universiteit Brussel, Brussels, Belgium
² Department of Chemical and Physical Health Risks, Sciensano, Brussels, Belgium
^P Presenting author

P114

ICCS best practice guidance document for serious eye damage and eye irritation assessment using new approach methodologies for substances in cosmetics and personal care products

Takayuki Abo¹, Els Adriaens², Nathalie Alépée³, Lauren Brown⁴, Alessandra Cavarzan⁵, Neepa Choksi⁴, Renato Ivan de Ávila⁶, Carolin Goebel⁷, Donna S. Macmillan^P8, Karsten R. Mewes⁹, Chelsea O’Driscoll^10
1 Kao Corporation, Japan
² Adriaens Consulting, Belgium
³ L’Oréal Research & Innovation, France
⁴ ToxStrategies LLC, USA
⁵ Reckitt Benckiser Italia S.P.A., Italy
⁶ Safety, Environmental and Regulatory Science (SERS), Unilever, UK
⁷ Beiersdorf AG, Germany
⁸ International Collaboration on Cosmetics Safety, USA
⁹ Henkel, AG & Co. KGaA, Germany
¹⁰ Procter & Gamble, USA
^P Presenting author

P115

Structured research, adequate reproducibility: a digital approach to managing the complexity of in vitro studies

Iasmina Marcovici^P1
1 INVITRIO SCIENTIFIC SRL, Romania
^P Presenting author

Go back

Local toxicity testing (safety and efficacy)

P116

Optimizing keratinocyte spheroid formation: efficiency and morphological outcomes

Jéssica Nascimento da Silva  Pinto¹, Patrícia Prado  Augusto¹, Matheus de Souza  Alves¹, Júlia Luíza André  Santana¹, Wilson Dias  Segura¹, Meg Cristina de Castilho  Costa², Carolina Motter  Catarino², Patricia Santos Lopes^P1
1 Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil
² Pre-Clinical & Product Safety – Grupo Boticário, Brazil
^P Presenting author

P117

Fruits from Brazilian Cerrado as a potential source for application in cosmetics: in silico and in vitro analysis

Bruna Stephany Fernandes  Lopes¹, Larissa Silva Costa¹, Marcelo Dutra Duque¹, Vânia Rodrigues Leite-Silva^1,2, Newton Andreo-Filho¹, Patricia Santos Lopes^P1
1 Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau 210, Diadema 09913-030, Brazil
² Therapeutics Research Centre, The University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, Queensland 4102, Australia
^P Presenting author

P118

Nanocellulose as a food additive: assessing the toxicological implications of nanocellulose in the gastrointestinal tract.

Jia Yi Poh^P1, Magdiel Inggrid Setyawati¹, Kee Woei Ng^1,2
1 School of Materials Science and Engineering, Nanyang Technological University
² Nanyang Environment and Water Research Institute (NEWRI), Nanyang Technological University
^P Presenting author

P119

Comparison of in vitro skin sensitization strategy using a data set of cosmetic raw materials

Elise Roux¹, Frédéric Alby¹, Elodie Clouet¹, Emilie Floch^P1, Marie Pierre Gomez Berrada¹, Pierre Jacques Ferret^1
1 Safety Products Department, Pierre Fabre Dermo-Cosmétique, Toulouse, France
^P Presenting author

P120

An in vitro strategy using RhE to assess the tolerance and efficacy of personal care products based on a concrete example: a repair balm

Agathe DENIAU^P1, Manon FOURNIER¹, Adeline JOSSEAUME², Sophie CATOIRE², Adeline NALIN^1
1 Aroma-Zone
² CELL ALTERNATIV
^P Presenting author

P121

Epidermal repair after superficial burn: in vitro evaluation of reparative effects and soothing products

Adeline JOSSEAUME^P1, Sophie GOLDBLUM², Sophie CATOIRE^1
1 CELL ALTERNATIV
² PURESSENTIEL
^P Presenting author

P122

Evaluation of human blood as a sustainable alternative to bovine blood in the red blood cell (RBC) test for eye irritation assessment

Daniela Lange^P1, Andreas Schepky¹, Nadine Müller^1
1 Beiersdorf AG
^P Presenting author

P123

Investigation of the intestinal effects of dietary advanced glycation endproducts in an vitro model of the inflamed intestine

Angela A.M. Kämpfer^P1, Heleen A. van der Hout², Roel P.F. Schins^1,2, Misha F. Vrolijk^2
1 IUF – Leibniz Research Institute for Environmental Health
² Department of Pharmacology and Toxicology, Maastricht University
^P Presenting author

P124

Integrated transcriptomics as a new approach methodology (NAM) for predictive chemical safety and efficacy assessment in skin models

Thomas Darde^P1, Pamela El Azzi², Frédéric Chalmel^3
1 SciLicium
² Wepredic
³ INSERM U1085 – IRSET
^P Presenting author

P125

Further development of the in vitro photoassay using THP-1 cells to identify phototoxic chemicals

Adriana S Maddaleno¹, Laia Guardia-Escoté², M. Pilar Vinardell¹, Elisabet Teixidó², Montse Mitjans^P1
1 Dpt. Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’alimentació, Universitat de Barcelona
² Departament de Farmacologia, Toxicologia i Química Terapèutica,  Facultat de Farmàcia i Ciències de l’alimentació, Universitat de Barcelona
^P Presenting author

P126

Empowering discovery of safe and effective drugs targeting cystic fibrosis infections by using new Candida albicans-CF zebrafish model

Aleksandar Pavic^P1,2, Natasa  Radakovic¹, Nikola  Plackic¹, Aleksandar Sovtic³, Fabio  Zobi⁴, Joachim  Morschhäuser^5
1 Institute of Molecular Genetics and Genetic Engineering University of Belgrade
² L. S. Skaggs Pharmacy Institute Pharmacology & Toxicology College of Pharmacy – University of Utah
³ Institute of Mother and Child Healthcare of Serbia “Dr Vukan Čupić”
⁴ Université de Fribourg  Faculté des Sciences et de Médecine
⁵ Institut für Molekulare Infektionsbiologie Universität Würzburg
^P Presenting author

P127

Multimodal neurotoxicity profiling of pesticides using a human iPSC-based organ-on-chip platform

Raphaëlle Baumier¹, Jessica Rontard², Benoît Maisonneuve^P2, Guillaume Becker¹, Thibault Honegger^2
1 ANSES
² NETRI
^P Presenting author

P128

Development and optimization of an MCTT HCE™-based eye hazard identification assay

Nam-Hee Kang^P1, Soo In Lee¹, Joohwan Kim¹, Sun Wook Woo^1
1 Korean Center for the Validation of Alternative Methods (KoCVAM), Nonclinical Resource Research Division, National Institute of Food and Drug Safety Evaluation,  Ministry of Food and Drug Safety
^P Presenting author

P129

Establishment of a KeraSkin™ RhE-based skin sensitization test method

Sun Wook Woo¹, Nam-Hee Kang¹, HanBit Bong¹, Joohwan Kim¹, Rheeda Lee^P
1 Korean Center for the Validation of Alternative Methods (KoCVAM), Nonclinical Resource Research Division, National Institute of Food and Drug Safety Evaluation,  Ministry of Food and Drug Safety
^P Presenting author

P130

A reconstructed canine epidermis model as an alternative approach for local tolerance assessment in veterinary pharmacology and pet care

Jérôme Dellacasagrande^P1
1 QIMA Life Sciences
^P Presenting author

P131

Tissue-specific effect of antiparasitic substances. An in vitro approach.

David Hernández-Moreno^P1, Elsa Rodríguez-Somoza ², Marcos Perez-Lopez², M.Luisa Fernandez-Cruz^1
1 Department of Environment and Agronomy, National Institute for Agriculture and Food Research and Technology (INIA, CSIC). Madrid. Spain
² Toxicology Area, Veterinary School, University of Extremadura. Caceres. Spain
^P Presenting author

P132

Development of a 3D atopic dermatitis model using keraskin™ and its application in preclinical efficacy evaluation

Hye-jin Lee^P1, Eun-Ji Gwak¹, Geon-Hee  Lee¹, Su-Hyon  Lee^1
1 R&D Institute, Biosolution Co., Ltd., Seoul, Republic of Korea
^P Presenting author

P133

Toxicological evaluation of pristine MXene, polypyrrole-functionalized MXene, and MXene–graphene hybrids using cellular and computational models

Dalia de la Fuente-Vivas^P1, Sergio de la Huerta^1,2, Julia Rodríguez-Vinagre¹, David Palacios^1,3, Santiago Aparicio^1,2, Laura Gómez-Cuadrado^1
1 International Research Center in Critical Raw Materials for Advanced Industrial Technologies (ICCRAM), Universidad de Burgos, Centro de I+D+I, Burgos, Spain
² Department of Chemistry, Universidad de Burgos, Burgos 09001, Spain
³ Department of Biotechnology and Food Science, Universidad de Burgos, Burgos 09001, Spain
^P Presenting author

P134

Comparative hepatotoxicity assessment of a magnetic colloidal suspension containing oleic acid-double functionalized iron oxide nanoparticles in 2D and 3D liver models

Iasmina Marcovici^P1,2, Elena-Alina Moacă^1,2, Cristina-Adriana Dehelean^1,2
1 Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.
² Research Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.
^P Presenting author

P135

Skin corrosion/irritation: evaluating the readiness of NAMs for regulatory decision-making

B. de Sá Calçada^P1, M. E. Schutte², T. Holmes¹, S. Wright-Williams³, J. Mehta^3
1 ADAMA Deutschland GmbH, Edmund-Rumppler-Str. 6, 51149 Cologne, Germany
² ADAMA Northern Europe BV., Arnhemseweg 87, Leusden, the Netherlands
³ ADAMA Agricultural Solutions UK, Arlington Business Park, Theale, Reading, United Kingdon
^P Presenting author

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Models, biomarkers and assays for developmental toxicity

P136

Advancing developmental toxicity testing with species-specific in vitro methods

Jing Fang^P1, Tom  Zwetsloot¹, José M. Horcas-Nieto¹, Amer Jamalpoor^1
1 Toxys B.V., Leiden BioScience Park, Oegstgeest, The Netherlands
^P Presenting author

P137

Developmental neurotoxicity of dibutyl phthalate and its primary metabolite mono-n-butyl phthalate in zebrafish embryo model

Suyeon Lee¹, Sangwoo Lee^P1, Woo-Keun Kim^1
1 Korea Institue of Toxicology
^P Presenting author

P138

From DNT to DART: leveraging a NAM success story

Julia Alicia Boos^P1, Angela Bearth^1,2, Lucia  Selfa Aspiroz³, Ellen Fritsche^1
1 SCAHT – Swiss Centre of Applied Human Toxicology
² HF Partners GmbH
³ European Commission, Joint Research Centre (JRC)
^P Presenting author

P139

Human stem cell-derived osteoblasts for in vitro assessment of skeletal malformation

Connor Philippo^P1, José M. Horcas-Nieto¹, Giel  Hendriks¹, Amer Jamalpoor^1
1 Toxys B.V.
^P Presenting author

P140

Qualification and quantification of a novel human in vitro syncytiotrophoblastic barrier: A predictive tool for maternal exposure.

Romain Grall^P1, Mickael Le Balch¹, Theo Sizorn-Gueguen¹, Sylvie Emery¹, Anne Riu¹, Kevin Cache¹, Gladys Ouedraogo¹, Matthew Burbank^1
1 L’Oréal Research & Innovation, Aulnay-sous-Bois, France 
^P Presenting author

P141

Integrating hPSC-derived monolayer and brain-on-chip models as new approach methodologies for developmental neurotoxicity testing.

Marta Cervera-Sospedra^P1,2, Clelia Introna^1,2, Georgina Bombau^1,2, Cinta Gomis^1,2, Marc Estarellas^1,2, Josep Samitier³, Josep M. Canals^1,2
1 Laboratory of Stem Cells and Regenerative Medicine, Department of Biomedical Sciences; and Creatio, production and validation center of advanced therapies, Faculty of Medicine and Health Sciences; and Institute of Neurosciences, University of Barcelona, Barcelona, Spain.
² Fundació de Recerca Clínic Barcelona-Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
³ Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
^P Presenting author

P142

Development of an in vitro placental barrier model to assess the teratogenicity potential of chemical compounds.

Giovana Bento^P1, Nathalia  Indolfo ¹, Melissa  Ganzerla¹, Juliana Costa¹, Graziele  Sousa¹, Ana Clara Stival², Marize  Valadares^2
1 Natura
² ToxIn Lab- Federal University of Goiás
^P Presenting author

P143

Metabolomics and lipidomics for neurotoxicity assessment in human 3D brain spheres: an example with glycol ether solvents

Isabel Meister^P1,2, Mathieu Galmiche^1,2, David Lopez Rodriguez^3,4,2, Julien Boccard^1,2, David Pamies^3,2, Tatjana Saric^5,6, Estelle Maret⁶, Noéline Héritier^3,2, Massimo Frangiamone^3,2, Aurélien Thomas^5,6, Nancy Hopf⁷, Marie-Gabrielle Zurich^3,2, Serge Rudaz^1,2
1 School of Pharmaceutical Sciences, University of Geneva, Switzerland
² Swiss Center for Applied Human Toxicology
³ Department of Biomedical Sciences, University of Lausanne, Switzerland
⁴ Institute of Earth Surface Dynamics, University of Lausanne, Switzerland
⁵ Unit of Forensic Toxicology and Chemistry, CURML, Lausanne and Geneva University Hospitals, Switzerland
⁶ Unit of Toxicology, CURML, Faculty of Biology and Medicine, University of Lausanne, Switzerland
⁷ Unisanté, Center for Primary Care and Public Health and University of Lausanne, Switzerland
^P Presenting author

P144

A regulatory-relevant human placental model to identify early endocrine and molecular disruptions

Beatrice Anna Brugger^P1, Caroline Buff¹, Jack Morikka², Lorenzo Campini², Dario Greco², Peter Wick¹, Tina Buerki-Thurnherr^1
1 Nanomaterials in Health Lab, Empa St. Gallen, Switzerland
² Faculty of Medicine and Health Technology, Tampere University, Finland
^P Presenting author

P145

Non-exhaust particles from electric vehicles: evaluation in human skin and lung models within the LIFE-NEEVE project.

Verónica Ruiz-Torres^P1, María José  Periáñez-Ordóñez², Alicia  Castillo-Rivas², Paloma  Álvarez-Mateos², Dominik  Soare³, Kenji  Kondo ³, Leonid  Japs³, Marie  Lens ³, Matthias  Schröder³, Peter  Lienerth³, María  Herranz-López¹, Vicente  Micol¹, Enrique  Barrajón^1
1 Institute of Biomedical Research of Elche (IDiBE), Miguel Hernández University (UMH), Elche, Spain.
² Department of Chemical Engineering, Faculty of Chemistry, University of Seville, Spain.
³ HORIBA Europe GmbH, Hans-Mess-Str. 6, 61440 Oberursel, Germany
^P Presenting author

P146

Cross-species human and primate iPSC-based NAMs for in vitro developmental toxicity assessment of targeted protein degraders

Fabian P Suchy^P1,2, Katie Kotok^1,3, Lena Brücker⁴, Lucia Ramirez¹, Philip Hewitt⁴, Morteza Roodgar^1,2
1 Exir Bio, Menlo Park, CA 94025, USA.
² Stanford University, School of Medicine, Stanford University, Stanford, CA 94305, USA.
³ San José State University, San Jose, CA 95192, USA.
⁴ Merck Healthcare KGaA, Darmstadt, 64293, Germany. 
^P Presenting author

P147

Evaluation of neurodevelopmental disruptions induced by emerging Bisphenol analogues using zebrafish embryo

Suyeon Lee^P1,2, Sangwoo Lee^1,2, Woo-Keun Kim^1,2
1 Korea Institute of Toxicology
² University of Science & Technology
^P Presenting author

P148

Development of regulatory toxicity indicators for biocides: bridging transgenic zebrafish imaging with AOP-based human health risk assessment

Donggon Yoo^P1,2, Sangwoo Lee^1,2, Woo-Keun Kim^1,2
1 Human and Environmental Toxicology, University of Science and Technology, Daejeon 34113, Republic of Korea.
² Departmemt of Predictive Toxicology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea.
^P Presenting author

P149

High-throughput quantitative analysis of PLP1-positive oligodendrocytes in a 3D human iPSC-derived brain model for chemical screening

Federica Lamberto^P1, Andrea  Aranda Prats¹, Lena  Smirnova², David Pamies^1
1 Department of Biomedical Sciences, Faculty of Biology and Medicine, University of Lausanne, Switzerland
² Center for Alternatives to Animal Testing, Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States
^P Presenting author

P150

The devTOX quickPredict™ assay: blinded study demonstrates high inter-laboratory reproducibility for in vitro developmental toxicity NAM

Darren Kidd^P1, Daniel Bramham¹, Irina Komjarova², Craig Griffiths², Paul Bushdid³, Jessica Palmer⁴, Elizabeth Donley^4
1 Labcorp, Harrogate, UK
² Labcorp, Huntingdon, UK
³ Labcorp, Greenfield, IN, USA
⁴ Stemina Biomarker Discovery Inc, Madison, WI, USA
^P Presenting author

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Models, biomarkers and assays for endocrine disruption

P151

Development and validation of a sodium iodine symporter (NIS) inhibition assay using a hNIS overexpressing cell model

Sjoerd Verkaart^P1
1 PhD
^P Presenting author

P152

Endocrine disrupting properties of water soluble metal working fluids: effects on steroid hormone production and receptor transcriptional activities.

Christian Darne¹, Mylène Lorcin¹, Marie Perceau¹, Dieynaba Ndiaye¹, Laurent Gaté^P1
1 Institut National de Recherche et de Sécurité, Département Toxicologie et Biométrologie, 1 rue du Morvan, 54519 Vandoeuvre-lès-Nancy, France
^P Presenting author

P153

C. Elegans as an innovative biosensor for combined toxicity in surface water: exploratory insights from the INNOWACE project

Amra Milasinovic^P1, Nienke  Stigter¹, Roberta  Hofman-Caris², Cyrille A.M.  Krul¹, Raymond H.H.  Pieters^3
1 HU University of Applied Sciences, Research Group Innovative Testing in Life Sciences & Chemistry, Utrecht, The Netherlands
² KWR Water Research Institute, Nieuwegein, The Netherlands
³ Utrecht University, Institute for Risk Assessment Sciences, Utrecht, The Netherlands
^P Presenting author

P154

Towards serum-free, hormone-controlled culture conditions for in vitro steroidogenesis assays

Eliška Řehůřková¹, Lucie Bízová¹, Lola Bajard¹, Iva Sovadinová^P1
1 RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic 
^P Presenting author

P155

Refining predictive transcript markers for liver steatosis in metabolism-disrupting compound screening

Dajana Lichtenstein^P1, Heike Sprenger¹, Albert Braeuning^1
1 German Federal Institute for Risk Assessment
^P Presenting author

P156

NAM-based assay panel for evaluating compound effects on thyroid hormone synthesis

Clémence Budin^P1, Harrie Besselink¹, Tjalf de Boer¹, Abraham Brouwer^1
1 BioDetection Systems BV
^P Presenting author

P157

Chlorpyrifos induces dimorphic effects in immortalized hypothalamic murine neurons in presence of physiological concentrations of sexual hormones

Irene Masciola^P1, Gabriele Lori¹, Marialuisa Casella¹, Antonella Tinari¹, Sabrina Tait^1
1 Istituto Superiore di Sanità
^P Presenting author

P158

Thyroid hormone-disrupting potential of wastewater treatment plant effluents across Europe: insights from an in vitro bioassay battery

Marie Smutná^P1, Puja Kumari¹, Zuzana Toušová¹, Saskia Finckh², Jiří Novák¹, Werner Brack², Klára Hilscherová^1
1 RECETOX, Masaryk University, Brno, Czech Republic
² Department of Effect-Directed Analysis, UFZ – Helmholtz Centre for Environmental Research, Leipzig, Germany
^P Presenting author

P159

Advancing mechanistic investigations of thyroid toxicity through new analytical parameters in a 3D RAT thyroid model

Nicolas Orsini^P1, Julia Kühnlenz¹, Helen Tinwell^1
1 Bayer CropScience, Human Safety, Sophia Antipolis, France
^P Presenting author

P160

Endocrine-disrupting potential of pollutant mixtures in settled dust samples from households with little children from eight European countries

Jiri Novak^P1, Marie Smutna¹, Lisa Melymuk¹, Klara Hilscherova^1
1 RECETOX, Masaryk University, Brno, Czech Republic
^P Presenting author

P161

Integrated zebrafish NAMs for endocrine disruption: mechanistic insights into thyroid and estrogen pathways

Andrea Weiner¹, Ander Arbelaiz¹, Mikel Queralt¹, Arantza Muriana^P1
1 BBD BioPhenix SL – Biobide
^P Presenting author

P162

Testicular organoids: a potential model to study the impact of endocrine-disrupting chemicals on steroidogenesis

Katerina Papageorgiou^P1,2, Samuel Madureira Silva¹, Andrea Errico³, Mikael Pedersen⁴, Ellen Goossens¹, Tamara Vanhaecke², Yoni Baert^2
1 Genetics, Reproduction and Development research group, Biology of the Testis (BITE) laboratory, Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium.
² In Vitro Toxicology and Dermato-cosmetology (IVTD) laboratory, Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium.
³ University of Verona; Department of Neurosciences, Biomedicine and Movement Sciences; 37124 Verona, Italy.
⁴ National Food Institute, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
^P Presenting author

P163

Hazard assessment of endocrine disruption potential using new alternative methods[SV1.1]: integration of in vitro thyroid peroxidase inhibition potency and metabolic fate of 3-amino-2,6-dimethylphenol explains its lack of thyroid toxicity in vivo

Sebastian Oskar Jarzina^P1, Sjoerd  Verkaart², Joris van den Elzen², Nuria Pineiro Costas², Manuela Pavan³, Christian  Novello³, Brunhilde Blömeke⁴, Mira Wenker², Carsten Goebel¹, Karma Claire Fussell ^1
1 Wella Company
² Charles River Lab.
³ Innovatune Srl
⁴ University Trier
^P Presenting author

P164

Diverging endocrine disruption potential of resorcinol and paracetamol: thyroid peroxidase inhibition potency and metabolic fate differences under pharmaceutical and cosmetic use explain their contrasting endocrine disruption capabilities

Sebastian Oskar Jarzina^P1, Sjoerd Verkaart², Joris van den Elzen², Manuela  Pavan³, Christian Novello³, Brunhilde  Blömeke⁴, Mira Wenker², Carsten Goebel¹, Karma Claire Fussell^1
1 Wella Company
² Charles River Lab.
³ Innovatune Srl
⁴ University Trier
^P Presenting author

P165

Monitoring PFAS and estrogenic bioactivity in source water using ERα- and PFAS-CALUX: A NAMs and ecosystem approach

Giulia De Negri Atanasio^P1,2, Lorenzo Dondero^1,2, Francesca Tardanico^1,2, Matteo Sergiampietri¹, Erica Lertora^1,2,3, Giacomo Rosa¹, Andrea Costa¹, Sebastiano Salvidio¹, Elena  Grasselli^1,2,4
1 Department of Earth, Environment and Life Science, University of Genoa, Genova, Italy
² Interuniversity Center for the Promotion of 3R Principles in Teaching and Research (Centro3R), Pisa, Italy
³ Angel Consulting S.a.s, Milano, Italy
⁴ Biostructures and Biosystems National Institute, Roma, Italy
^P Presenting author

P166

Generation of a historical control database, and acceptance and interpretation criteria for an in vitro comparative T4-glucuronidation assay using primary human and RAT hepatocyte (TruVivo®) for determination of human relevance of liver enzyme-mediated thyroid effects

Ahtasham Raza¹, Lauren Kent^P2, Marco Corvaro³, Jinlang Dong¹, Justin Brown¹, Suhair Al Janabi¹, Stephanie Kellum¹, Ed LeCluyse⁴, Jessica LaRocca⁵, Raja Settivari¹, Shadia Catalano^1
1 Corteva Agriscience, Newark, Delaware, USA
² Corteva Agriscience, Milton, United Kingdom
³ Corteva Agriscience, Rome, Italy
⁴ LifeNet Health LifeSciences, Research Triangle Park, North Carolina, USA
⁵ Corteva Agriscience, Indianapolis, Indiana, USA
^P Presenting author

P167

Functional and safety assessment of estradiol and phytoestrogens in human epidermal models

Francesca Rispo¹, Giulia  De Negri Atanasio¹, Ilaria  Demori², Francesca Tardanico¹, Giosuè Costa³, Emanuela Marchese³, Simón  Perera del Rosario^4,5, Eva  Serrano-Candelas⁴, Martina  Palomino-Schätzlein⁶, Lorenzo Dondero¹, Annalisa Salis⁷, Gianluca Damonte⁷, Erica Lertora^1,8, Matteo  Sergiampietri^1,8, Sara Ferrando¹, Silvia Letasiova⁹, Jan Markus⁹, Matteo  Zanotti-Russo⁸, Elena Grasselli^P1,10,11
1 Department of Earth, Environment and Life Science, University of Genoa, Genova, Italy
² Department of Pharmacy, University of Genoa, Genova, Italy
³ Department of Health Sciences, University “Magna Graecia”, Catanzaro, Italy
⁴ ProtoQSAR SL, Centro Europeo de Empresas Innovadoras, Parque Tecnológico de Valencia, Valencia, Spain
⁵ Departament de Medicina i Ciències de la Vida, Institut de Biologia Evolutiva (CSIC-UPF), Universitat Pompeu Fabra, Barcelona, Spain
⁶ Moldrug AI Systems S.L, Valencia, Spain
⁷ Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy
⁸ Angel Consulting Srl, Milano, Italy
⁹ MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
¹⁰ Interuniversity Center for the Promotion of 3R Principles in Teaching and Research (Centro 3R), Pisa, Italy
¹¹ Biostructures and Biosystems National Institute, Roma, Italy
^P Presenting author

P168

Luteolin promotes steroidogenesis in I-10 mouse leydig cells exposed to atrazine

Maryam Dawod^P1, Ali Siddiqi¹, Pengli Bu^1
1 Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John’s University, Queens, 11439, NY, USA
^P Presenting author

P169

Multiple mechanisms of AhR/CAR/PXR activators in thyroid (T-) modality: an in vitro perspective on human relevance

Audrey BAZE^P1, Betty Ory¹, Lucille Wiss¹, Amélie Schäfer¹, Cassandra Chanel¹, Corinne Guicheney², Klaus Biemel³, Laure Asselin^1
1 KaLy-Cell SAS (GBA Group)
² GBA Pharma
³ Pharmacelsus GmbH (GBA Group)
^P Presenting author

P170

Interlaboratory validation study of a mineralocorticoid receptor transactivation assay (MRTA) to identify endocrine disruptors in an in vitro setting

Kevin Schlüppmann^P1, Andrea Rivero-Arze¹, Pauline Lelandais¹, Patrick Balaguer², Torben Österlund^1
1 Pepper, Paris, France
² Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, ICM, Univ Montpellier, Montpellier, France
^P Presenting author

P171

Validation project of a fluorescent FITC-T4 transthyretin competitive binding assay

Nathalie Belot^P1, Pauline Lelandais¹, Timo Hamers², Toine Bovee³, Torben Österlund^1
1 Pepper
² Vrije Universiteit Amsterdam (VU), NL
³ Wageningen Food Safety Research (WFSR), NL
^P Presenting author

P172

Enhancement project of the LC-MS/MS based steroidogenesis profiling assay (OECD TG456)

Nathalie Belot^P1, Nina Hambruch², Torben Österlund^1
1 Pepper
² BASF
^P Presenting author

P173

Chemical mixture driven androgen receptor disruption

Nikolina Peranić^P1, Lucija Božičević¹, Korinna Altmann², Jana Hildebrandt², Ivana Vinković Vrček^1
1 Institute for Medical Research and Occupational Health
² Bundesanstalt für Materialforschung und -prüfung
^P Presenting author

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Models, biomarkers and assays for systemic and immune toxicity

P174

In vitro genotoxicity assessment of chitin intended for use in the food industry

Antía Lestido-Cardama¹, Leticia Diez-Quijada¹, María Puerto^P1, Ángeles Jos^1
1 Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain.
^P Presenting author

P175

Evaluating assay sensitivity in NGRA: A comparative study of cellular stress panels

Adam Wood^P1, Eva de Tombe², Tamara Meijer², Sharon Scott¹, Bensu Tangil², Bas ter Braak², Amer Jamalpoor^2
1 Unilever
² Toxys
^P Presenting author

P176

Integrated in vitro approach for immunotoxicity assessment of synthetic amorphous silica nanomaterials

Amandine Lê^P1,2, Emilie  Brun ³, Ambre  Jacquet¹, Cédric  Féral-Martin², Jacques-Aurélien  Sergent⁴, Marc  Pallardy¹, Armelle  Biola-Vidamment^1
1 Université Paris-Saclay, Inserm, Inflammation, Microbiome and Immunosurveillance, Faculté de Pharmacie, 91 400, Orsay, France
² Solvay, 93300, Aubervilliers, France.
³ Université Paris-Saclay, CNRS, Institut de Chimie Physique, 91405, Orsay, France.
⁴ Solvay, Toxicological and Environmental Risk Assessment Unit, 1120 Neder-Over-Heembeek, Belgium
^P Presenting author

P177

Toxicity and vector role of micro- and nanoplastics with pesticide residues in human cell line models

Orlagh Gormley^P1, Ali Can¹, Gary Hardiman^1
1 School of Biological Sciences and Institute for Global Food Security, Queen’s University Belfast, 19 Chlorine Gardens, Belfast, BT9 5DL, Northern Ireland, UK
^P Presenting author

P178

Investigation of the potential of Bisphenol A substitutes to induce allergic contact sensitization using OECD defined approaches

Victor J. Johnson^P1, Travis Gulledge², Michael I. Luster¹, Gary R. Burleson¹, Florence G. Burleson^1
1 Burleson Research Technologies, Inc., Morrisville, NC, USA
² Torque Bio, Inc., Durham, NC, USA
^P Presenting author

P179

Developing a 3D multi component human skin model for vitiligo biomarker identification

MD ZOBAER HASAN^P1,2, David Openshaw¹, Hirofumi Nakanishi², Kiichiro Sohara², Tetsuo Furuno², Craig Murdoch¹, Helen Colley^1
1 University of Sheffield
² Rohto Pharmaceutical
^P Presenting author

P180

Setting up new approach methods to study B cell immunotoxicity using peripheral blood mononuclear cells

Wendy Jansen Holleboom¹, Aafke WF Janssen^P1
1 Wageningen Food Safety Research, part of Wageningen University & Research, Wageningen, The Netherlands
^P Presenting author

P181

Recapitulating immune-driven hepatotoxicity using a liver microphysiological platform

Justina Then^P1, Isavella Georgiades¹, Emily Richardson¹, Tomasz Kostrzewski^1
1 CN Bio Innovations Ltd, Science Park, Cambridge, United Kingdom
^P Presenting author

P182

Development and characterization of an immunocompetent and vascularized skin model

Ambre Alléon^P1,2, Annia Perez-Riveron¹, Pualani Ateni¹, Marina Simon², Christian Poinsot², Edith Filaire², Vincent Flacher^1
1 Laboratory CNRS UPR3572/I2CT Immunology, Immunopathology and Therapeutic Chemistry, Strasbourg Drug Discovery and Development Institute (IMS), Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
² Groupe Icare, Biopôle Clermont-Limagne, Saint Beauzire, France.
^P Presenting author

P183

Comparative analysis of cellular respones to selected carcinogens in 3D HEPG2 monoculture and HEPG2/kupffer cell co-culture models

Tim Ravnjak^P1,2, Gillian  Conway³, Michael  Burgum³, Shareen  Doak³, Bojana Žegura^1,2
1 National Institute of Biology, Department of Genetic Toxicology and Cancer Biology, Večna pot 121, Ljubljana, Slovenia
² Jozef Stefan International Postgraduate School, Jamova cesta 39, Ljubljana, Slovenia
³ In Vitro Toxicology Group, Faculty of Medicine, Health and Life Sciences, Swansea University Medical School, Swansea University, SA2 8PP, UK
^P Presenting author

P184

Biodegradable plastics: contaminants of emerging concern

Cigdem Erdogan^P1, Ali Can¹, Gary  Hardiman^1
1 Queen’s University Belfast
^P Presenting author

P185

Linking structural similarity to toxicogenomic profiles: transcriptomic read-across of flavonoids

Markus Bruell^P1, Silja Voß¹, Rita Fernandes¹, Andreas Schepky¹, Johanna Ebmeyer^1
1 Beiersdorf AG
^P Presenting author

P186

Assessment of the sensitizing potential of chemicals and proteins, alone and in mixtures, using the bmdc model

Z. Salmon^P1,2, S. Jacquenet², A. Coiscaud², J. Mathiot², S. Muller², M. Mourot-Bousquenaud², S. Kerdine-Römer¹, F. Battais^2
1 Université Paris-Saclay, Inserm, Inflammation microbiome immunosurveillance, Team ITALI – Immunotoxicology, Allergy and Immunogenicity 91400, ORSAY, France
² French Research and Safety Institute for the Prevention of Occupational Accidents and Disease (INRS, « Immunology, sensitization and allergies » laboratory, Toxicology and Biomonitoring Division, Vandoeuvre-lès-Nancy, France
^P Presenting author

P187

When safe isn’t safe together: co-exposure to individually non-toxic compounds alters liver HEPG2 spheroid functional integrity

P188

Comparative roles of CYP2E1 in isoniazid- and iproniazid-induced hepatotoxicity

Yasuhiro Masubuchi^P1, Kyohei Miyauchi^1
1 Faculty of Pharmaceutical Sciences, Chiba Institute of Science
^P Presenting author

P189

Mechanistic investigation of the immunotoxicity of synthetic amorphous silica nanomaterials using new approach methodologies

Ambre Jacquet^P1, Amandine Lê ¹, Agata Nawrotek Maalouf², Marc Pallardy¹, Mahel Zeghouf ², Armelle  Biola-Vidamment^1
1 Université Paris-Saclay, INSERM, Inflammation, Microbiome and Immunosurveillance, Orsay, France
² ENS Paris-Saclay, CNRS, Laboratoire de Biologie et Pharmacologie Appliquée, Gif-Sur-Yvette, France
^P Presenting author

P190

Development of a large-pore cell-based in vitro intestinal model for size-dependent microplastic transport and safety assessment

Yicheng Wei^P1, Shohei Kaneko¹, Hyunjin Choi², Takeshi Katsuda¹, Masaki Nishikawa¹, Yasuyuki Sakai^1
1 Graduate School of Engineering, The University of Tokyo
² Department of Bio and Brain Engineering, Daejeon, Korea
^P Presenting author

P191

Ex vivo nonclinical system using fresh circulating human whole blood (id.flow®) for evaluating immunotoxicity

Helena Laven¹, Erika Fletcher¹, Gunilla Törnqvist¹, Louise Nilsson¹, Alexander Kele¹, Sakthi Srinivasan^P1, Sara Mangsbo^1
1 Immuneed AB, Uppsala, Sweden
^P Presenting author

P192

Predictive and mechanistic immunotoxicology using single-cell metabolomics

Jeany Delafiori¹, Mohammed Shahraz², Katja Ovchinnikova¹, Shawn Owens¹, Theodore Alexandrov^P1,2
1 DeepCyte, Inc.
² Department of Pharmacology, University of California San Diego
^P Presenting author

P193

Integrating complementary in vitro endpoints reveals mitochondrial and oxidative effects of PAβN beyond standard viability assays

Elodie OLIVIER^P1, Hilal WEHBI², Gilles PHAN², Isabelle BROUTIN^2
1 Université Paris Cité, UMR CNRS 8038 C-TAC, Paris, France
² Université Paris Cité, UMR CNRS 8038, Paris, France
^P Presenting author

P194

Testing immunomodulatory effects (IMOC) of micro-and nanoplastic particles (MNP) and isothiazolinones in human monocytic cells

Sebastian  Wolf^1,2, Antonia Koerber^1,3, Hermann-Josef Thierse^P1,2
1 German Federal Institute for Risk Assessment, Department for Chemicals and Product Safety, Berlin, Germany
² Heidelberg University, Medical Faculty Mannheim, Mannheim, Germany
³ Freie Universität Berlin, Institute of Biochemistry, Berlin, Germany University
^P Presenting author

P195

A combined comet assay and viability strategy for phototoxicity and photogenotoxicity screening in HaCaT cells

Elisabet Teixidó^P1, Laura Pous¹, Adrianna S Maddaleno², Laia Guardia-Escoté¹, Maria Pilar  Vinardell², Montse  Mitjans^2
1 GRET – Toxicologia, Departament de Farmacologia, Toxicologia i Química Terapèutica, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona
² CEREX – Fisiologia, Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona
^P Presenting author

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Non-animal methods for safety testing of biopharmaceuticals/biotherapies/vaccines

P196

Development of human iPSC-derived hepatocytes for drug discovery, translational research and toxicity testing

Tom Harris-Brown^P1, Gianmarco Mastrogiovanni², Sabitri Ghimire², Roxana Piscupescu², Jethro Hundling Hundling², Nick  James², Victoria Cornelius², Stefan Milde², Andrew Knights², Val  Yianni², Ben Newman Newman², Karl Firth², Will Bernard^2
1 bit.bio
² bit.bio
^P Presenting author

P197

Development and validation of a human reporter cell-based assay for mitochondrial toxicity assessment

Tamara Meijer^P1, Bensu Tangil¹, Bas ter Braak¹, Eva de Tombe¹, Sharon Scott², Adam Wood², Amer Jamalpoor^1
1 Toxys B.V., Leiden Bio Science Park, The Netherlands
² Unilever R&D, SERS, Sharnbrook, United Kingdom
^P Presenting author

P198

Phenotypic screening platforms for neurotoxicity and neurorepair in CNS drug safety assessment

Gurvan   Mahe ¹, Jeroen   Esselink ¹, Mathew Hembury ¹, David Károly Ketskeméty¹, Mariana Pliego Caballero¹, Maria Blanca Torroba¹, Jody  van Offenbeek¹, Kimberly Lo¹, Cindy  J.M. Looman¹, Mark Musters¹, Malika Bsibsi¹, Laila Ritsma¹, Ludovico Buti¹, Andre van Marle¹, Sabrina de Munnik¹, Peter Imre Racz^P1, Marijn  Vlaming^1
1 Charles River Laboratories
^P Presenting author

P199

Preclinical de-risking of cell therapy products

Sabrina de Munnik¹, Benita Quist¹, Henry  Leonard¹, Louise Francois¹, Monique Hazenoot¹, Monika Hiller¹, Rhiannon Shea¹, Lauren Schewitz-Bowers¹, Daniel  Rocca¹, Robert  Nunan¹, Louise Brackenbury¹, Mark Aspinall O’Dea¹, Roxana Redis¹, Gemma Moiset¹, Peter Imre Racz^P1
1 Charles River Laboratories
^P Presenting author

P200

Skimune® a powerful human-centric platform for the identification of t cell engager immunotoxicity

Steven O’Reilly^P1, Katie Smith¹, Abbas Ishaq¹, Anne Dickinson^1
1 Alcyomics
^P Presenting author

P201

Non human primate in vitro NAMs: a translational platform to complement human NAMs for efficient drug safety evaluation

Lucia Ramirez¹, Fabian P.  Suchy ^1,2, Laura Rijns¹, Morteza Roodgar^P1,2
1 Exir Bio, Menlo Park, CA 94025, USA.
² Stanford University, School of Medicine, Stanford University, Stanford, CA 94305, USA.
^P Presenting author

P202

Metabolic and cytotoxic readouts differentiate responses of 2D and 3D in vitro pancreatic ductal adenocarcinoma models to 5-fluorouracyl and paclitaxel

Mateo Celinić^P1, Nikolina Peranić¹, Lucija Božičević¹, Nikolina Kalčec¹, Ivana Vinković Vrček^1
1 Institute for Medical Research and Occupational Health
^P Presenting author

P203

Multicellular human intestinal new approach methodologies (NAM) with machine learning for diarrhea risk strattification across small molecules and antibody drug conjugates (ADC)

Caridad Rosette^P1, Vivian Xiao Han¹, Sherwin Gervacio¹, Cole Toohey¹, Haylee Aidnick¹, Amar A. Sethi^1
1 VivoSim Labs, Inc.
^P Presenting author

P204

Multicellular human 3D liver new approach methodologies (NAM) for DILI risk assessment across small molecules and antibody drug conjugates

Mehrsa Mehrabi¹, Caridad Rosette^P1, Rachel Dudum¹, Payton Olson¹, Cole Toohey¹, Haylee Aidnik¹, Amar A. Sethi^1
1 VivoSim Labs Inc
^P Presenting author

P205

In vitro safety profiling of ADCs using an iPSC-derived human tissue screen to support FDA roadmap for biologics

Leo Smit¹, Katerina  Pitsa¹, Carola van Berkel¹, Sanne Holt^P1
1 Ncardia Services B.V.
^P Presenting author

P206

InVEST44™ is a cost-effective in vitro safety panel that identifies off-target effects in early stage drug discovery

Julian Wooltorton¹, Afsara Ahona¹, Nicole Barbacane¹, Alex D’Andrea¹, Justin Lansberry¹, Cindy Lay¹, Paige Miller¹, Luisa Duque^P1, John Ries¹, Jamin Steffen¹, Patricia Valentine^1
1 Reaction Biology Corporation, 1 Great Valley Parkway, Suite 2, Malvern PA 19355
^P Presenting author

P207

A one health view on global outlook and future strategies for aninal testing replacement for vaccines: a report on the AFSA-IABS international conference

Jan Turner^P1, Laura Viviani², Blaise Descampe³, Colleen Pike^1
1 Humane World for Animals
² SciEthiQ
³ Blaise Management
^P Presenting author

Go back

Organ-on-a-chip & Microphysiological Systems

P208

Evaluation of drug-induced liver toxicity of trovafloxacin and levofloxacin in a human microphysiological liver model

Tim Kaden^P1,2, Katja Graf¹, Knut  Rennert¹, Ruoya Li³, Alexander S.  Mosig², Martin Raasch^1
1 Dynamic42 GmbH, Jena, Germany
² Institute of Biochemistry II, Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany
³ Biopredic International, St Gregoire, France
^P Presenting author

P209

Qualification of the PhysioMimix DILI assay in the 3RsC-FDA cross-platform DILI project

Emily Richardson^P1, Justina Then¹, Isavella  Georgiades¹, Ovidiu  Novac¹, Kim Lachani¹, Tomasz Kostrzewski^1
1 CN Bio Innovations
^P Presenting author

P210

Scaling without compromise: liver MPS for lead optimization screens to investigative toxicology

Ovidiu Novac^P1, Emily Richardson², Clive  Roper³, Tomasz Kostrzewski^2
1 CN Bio Innovations
² CN Bio Innovations
³ Roper Toxicology Consulting Limited
^P Presenting author

P211

Integration of human hepatocytes and kupffer cells into an immune-competent liver organoids-on-a-chip model

Thaís Silva Pinto^P1, Kirsten Pondman², Maureen  M.J. Guichelaar³, Anja  Wilmes^1
1 Vrije Universiteit Amsterdam, Faculty of Science, AIMMS, Department of Chemistry and Pharmaceutical Sciences, Division of Molecular Toxicology. Amsterdam – The Netherlands
² University of Twente, Faculty of Electrical Engineering, Mathematics and Computer Science (EEMCS), BIOS lab-on-a-chip group. Enschede — The Netherlands
³ Medisch Spectrum Twente, Department of Gastroenterology & Hepatology. Enschede — The Netherlands
^P Presenting author

P212

Advancing NAMs with intestinal organoids: cross-species profiling of targeted protein degraders and hook effect dynamics

Camilla Ceroni^P1, Orlane Maloudi¹, Carole Pissot¹, Kuno Wuersch¹, Axel Vicart¹, Markus Linder¹, Katie Kubek-Luck^1
1 Novartis Pharma AG
^P Presenting author

P213

A human liver-on-chip platform integrating HepaRG cells and LSECs to model hepatic function

Ambre Giau¹, Hugo Coppens-Exandier^P1, Ana Mesic², Louna Blasco³, Thomas Darde⁴, Pamela Bachour El-Azzi¹, Agnès Jamin¹, Eva-Anne Subileau^1
1 Biopredic International
² ENS Paris – Saclay Laboratoire LUMIN (UMR9024)
³ Institut FEMTO-ST (UMR6174)
⁴ SciLicium
^P Presenting author

P214

Translational toxicology: adapting liver microphysiological system for cross-species evaluation of hepatotoxicity risk

Isavella Georgiades^P1, Dr. Justina Then¹, Dr. Ovidiu Novac¹, Dr. Emily Richardson¹, Dr. Tomasz Kostrzewski^1
1 CN Bio Innovations
^P Presenting author

P215

Impact of intestinal model complexity on deoxynivalenol-induced cytotoxicity: a comparative in vitro study

Martina Sánchez^1,2, Mercedes Taroncher^1,2, María José Ruiz^1,2, Veronica Zingales^P1,2
1 Toxicology Laboratory, Faculty of Pharmacy and Food Sciences, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
² Research group on Alternative Methods for the Determination of Toxic Effects and Risk Assessment of Contaminant and Mixture (RiskTox)
^P Presenting author

P216

Modeling CAR-T cell migration and efficacy in a microphysiological system

Rosanna Stolberg^P1, Dimitrios L. Wagner^2,3, Petra Reinke^2,3, Eva-Maria Dehne^1
1 TissUse GmbH, Berlin, Germany
² Charité – Universitätsmedizin Berlin, Berlin, Germany
³ Berlin Institute of Health (BIH) at Charité, Berlin, Germany
^P Presenting author

P217

Opportunities in toxicity testing of new drug modalities with 3D in vitro models

Sara Shafiei ¹, Xandor  Spijkers¹, Nienke Wevers¹, Kinga  Kosim¹, Indi Joore^P1, Brian Duggan¹, Vincent van Duinen¹, Flavio Bonanini¹, Henriette  Lanz¹, Dorota Kurek^1
1 MIMETAS B.V
^P Presenting author

P218

3D human intestinal organoids as a platform to assess compound-induced gastrointestinal toxicity

Tania  Martianez Canales¹, Tomasz  Cholewinski¹, Youri  van Elswijk ¹, Melissa  Zuiderwijk², Behnam  Amiri³, Christian  Maass³, Tessa  de Korte², Marijn  Vlaming¹, Berend van Meer², Ludovico Buti^P1
1 Charles River Labs
² Sync Biosystems – Demcon
³ MPSlabs, ESQlabs GmbH
^P Presenting author

P219

Predicting gastrointestinal toxicity using the assay-ready, primary-derived OrganoReady® colon organoid model

Sara Shafiei ¹, Brian Duggan¹, Melanie Cuijpers¹, Ka Yen Liu¹, Moran Morelli¹, Manon Haarmans¹, Indi Joore^P1, Kinga Kosim¹, Lenie van den Broek¹, Pleun Hombrink¹, Sebastiaan Trietsch^1
1 MIMETAS B.V, Oegstgeest, The Netherlands
^P Presenting author

P220

An air–liquid interface (ALI)-compatible, magnetically actuated platform for programmable cyclic stretch in human alveolar in vitro models

YU CHE LIN^1,1, Yasuyuki Sakai¹, Masaki NIshikawa¹, Hiroshi Katsuda^1
1 Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan
^P Presenting author

P221

Elucidating gut-liver interactions in first pass metabolism of drugs using on-chip perfused and direct oxygenated MPS (BioStellar™ plate)

Dhimas Kurniawan¹, Shion Kanehara², Yuna Osada², Aiko Mizuno³, Hirohito Taniguchi³, Taichi Aihara³, Katsuhiro Esashika⁴, Jingjing Yang⁴, Satoshi Yamasaki⁴, Yusuke Masuo², Yukio Kato², Nobuaki Shiraki⁵, Shoen Kume⁵, Kenta Shinha⁶, Hiroshi Kimura⁶, Masaki Nishikawa¹, Yasuyuki Sakai^P1
1 University of Tokyo
² Kanazawa University
³ Sumitomo Bakelite Co. Ltd.
⁴ Mitsui Chemicals Inc.
⁵ Institute of Science Tokyo
⁶ Tokai University
^P Presenting author

P222

Merging organoid and organ-on-chip technology to generate physiological models of ocular tissue

Svenja Wingerter^P1,2, Kevin Achberger³, Madalena Cipriano¹, Julia  Roosz⁴, Seyma Bektas Tercan¹, Johanna Chuchuy¹, Kaushambee Dave³, Simon J. Clark^2,5,6, Stefan Liebau³, Peter  Loskill^1,4
1 Department for Microphysiological Systems, Institute of Biomedical Engineering, Faculty of Medicine, Eberhard Karls University Tübingen, Tübingen, Germany
² Institute for Ophthalmic Research, Department for Ophthalmology, University Medical Center, Eberhard Karls University Tübingen, Tübingen, Germany
³ Institute of Neuroanatomy & Developmental Biology (INDB), Eberhard Karls University Tübingen, Tübingen, Germany
⁴ NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
⁵ Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK
⁶ University Eye Clinic, Eberhard Karls University of Tübingen, Tübingen, Germany
^P Presenting author

P223

Single cell printing enables adaptive tumor immunity interactions

Yuan Pang^1
1 Tsinghua University
^P Presenting author

P224

Microfluidic tumor-on-chip platforms for modeling tumor-immune cell interactions and patient-specific immunotherapy responses ex vivo

Julia Roosz^P1, Tengku Ibrahim Maulana², Nicole Anderle¹, André Rosa¹, Nathalia Ferreira³, Frauke Alves³, Peter Loskill^4,2,5
1 NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany
² Department for Microphysiological Systems, Institute of Biomedical Engineering, Faculty of Medicine, Eberhard Karls University Tübingen, Tübingen, Germany
³ Translational Molecular Imaging, Max-Planck-Institute For Multidisciplinary Sciences, Göttingen, Germany   
⁴ NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany 
⁵ 3R Center Tübingen for In Vitro Models and Alternatives to Animal Testing, Tübingen, Germany
^P Presenting author

P225

Amitraz induces cytotoxicity and functional alterations in neuronally differentiated SH-SY5Y spheroids

Felipe Franco-Campos^1,2, Raquel Penalva-Olcina^1,2, Mercedes Taroncher^1,2, Veronica Zingales^P1,2, Yelko Rodríguez-Carrasco^1,2, María-José Ruiz^1,2
1 Toxicology and Food Chemistry Laboratory, Faculty of Pharmacy. University of Valencia, Burjassot-Valencia, Spain
² Research Group in Alternative Methods for Determining Toxic Effects and Risk Assessment of Contaminants and Mixtures (RiskTox), University of Valencia, Burjassot-Valencia, Spain
^P Presenting author

P226

Engineering of cancer microenvironments with embedded interconnected microcapillary networks via in vitro magnetic assembly of endothelial-cell ‘seeds’.

Katarzyna Rojek¹, Paulina  Musolf¹, Jan Guzowski^P1
1 Institute of Physical Chemistry Polish Academy of Sciences
^P Presenting author

P227

Impact of intestinal model complexity on deoxynivalenol-induced cytotoxicity: a comparative in vitro study

Martina Sánchez ^P1,2, Mercedes Taroncher^1,2, María José Ruiz^1,2, Veronica Zingales^1,2
1 Toxicology Laboratory, Faculty of Pharmacy and Food Sciences, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
² Research group on Alternative Methods for the Determination of Toxic Effects and Risk Assessment of Contaminant and Mixture (RiskTox)
^P Presenting author

Go back

Other

P228

Convergence science: the 3Rs versus the NAMs

Thierry Decelle^P1
1 DCL solutions
^P Presenting author

P229

Investigation of the effects of reduced gravity on liver using new approach methodologies

Mathieu Vinken^P1
1 Vrije Universiteit Brussel
^P Presenting author

P230

Multi-level characterization of liver-based in vitro models and their application to food-derived chemicals

Alexandra-Costina Avîrvarei^P1, Amy  Maerten¹, Anouk  Verhoeven¹, Mathieu  Vinken¹, Julen  Sanz-Serrano^1
1 Department of Pharmaceutical and Pharmacological Sciences, Vrije Universiteit Brussel, Brussels, Belgium
^P Presenting author

P231

Overcoming systemic barriers toward animal-free research through collaboration and critical reflection

Katja Ten Cate^P1, Daniela Salvatori², Wim de Leeuw¹, Shalenie den Braver^1
1 Netherlands National Committee for the protection of animals used for scientific purposes (NCad)
² Ombion Centre for Animal-Free Biomedical Translation (CPBT)
^P Presenting author

P232

Challenges and opportunities in evaluation of in vivo and in vitro irritation

Klara Hammarstrand^P1, Crystal D’Silva¹, Paul Valaitis^1
1 Baxter Healthcare
^P Presenting author

P233

Development, characterisation and standardisation of in vitro airway models from the nasal cavity down to the alveoli

Samuel Constant^P1
1 Epithelix
^P Presenting author

P234

Development of an in vitro model incorporating adverse outcome pathways to assess aerosol effects on cellular mechanisms related to chronic obstructive pulmonary disease

David Smart^P1, Ian Crooks¹, Michael Hollings², Damien Breheny^1
1 British American Tobacco
² Labcorp UK
^P Presenting author

P235

From one lung to many tests: A NAM-compliant ex vivo lung slice model for inhalation toxicity assessment

Massimo Caruso^P1,2
1 University of Catania
² Center of Excellence for the acceleration of Harm Reduction (CoEHAR)
^P Presenting author

P236

Methods to assess delivered dose in air-liquid interface exposure systems

Tobias Krebs^P1
1 Vitrocell Systems GmbH
^P Presenting author

P237

Methods to assess delivered dose in air-liquid interface exposure systems.

Tobias Krebs^P1, Marco  Hebestreit¹, Christoph Schlager¹, Paul  Schumacher^1
1 Vitrocell Systems GmbH
^P Presenting author

P238

Enniatin B1 risk assessment in a new approach methodologies (NAMs) perspective

Mariam MAHDJOUB^P1, Charlène GENDRE¹, Estelle DUBREIL¹, Jérôme HENRI¹, Ludovic LE HEGARAT^1
1 ANSES French Agency for Food, Environmental and Occupational Health and Safety, Fougères Laboratory, France
^P Presenting author

P239

Mechanistic-based multi-omics approach to investigate the hepatotoxicity of nanomaterials: a case study on synthetic amorphous silica used as food additive

Sarah ALILAT^P1, Pierre-Jean FERRON¹, Andrea  HAASE², Nolwenn  GAUVREAU ¹, Francesco  CUBADDA³, Valérie  FESSARD¹, Thibaut  LÉGER ^1,4
1 French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Toxicology of Contaminants Unit, Fougères, France
² Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany
³ Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità-National Institute of Health, 00161 Rome, Italy
⁴ ProMetHEE proteomics and metabolomics facility, Fougères Laboratory, French Agency for Food, Environmental and Occupational Health & Safety (ANSES), 35306 Fougères CEDEX, France
^P Presenting author

P240

Hepatotoxicity of food ingredient synthetic amorphous silica (SAS) using NAMs: a case study from the NAMS4NANO project

Pierre-Jean Ferron^P1, Anne-Louise Blier¹, Sarah Alilat¹, Noa Oluyn², Eveline Verleysen², Jan Mast², Andrea Haase³, Francesco Cubadda⁴, Kevin Hogeveen¹, Thibaut Léger¹, Valérie Fessard^1
1 French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Fougères, FRANCE
² Sciensano, Groeselenbergstraat 99, 1180 Uccle, Belgium
³ German Federal Institute for Risk Assessment (BfR), Berlin, Germany
⁴ Istituto Superiore di Sanità – National Institute of Health (ISS), 00161 Rome, Italy
^P Presenting author

P241

In vitro study on inhibitory evaluation of hydrogen peroxide using infusions made from black and roselle tea

Alireza Ebadollahinatanzi^P1
1 Department of Medicinal Plants, Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
^P Presenting author

P242

Is the Ames screening test predictive for the regulatory Ames test?

Bas-jan van der Leede^P1, Walter Westerink¹, Mira Wenker^1
1 Charles River Laboratories Den Bosch Netherlands
^P Presenting author

P243

Detection of DNA cross-links in TK6 cells using a modified version of the in vitro alkaline comet assay

Walter Westerink^P1, Bas-jan van der Leede¹, Mira Wenker^1
1 Charles River Laboratories Den Bosch Netherlands
^P Presenting author

P244

Investigation of early metabolomic indicators of drug-induced liver injury(DILI) using extracellular untargeted metabolomics

Tae-Yong Jeong^P1
1 Hankuk University of Foreign Studies
^P Presenting author

P245

Hazard identification with alternative methodologies: a benzophenone-based photoinitiator disturbed bile acid homeostasis in human liver cells

Yu-Chi  Ho¹, Hui-Ti Tsai¹, Ming-Hsien Tsai¹, Ju-Hsin Hsu¹, Pinpin Lin^P1
1 National Institute of Environmental Health Sciences, National Health Research Institutes, Taiwan
^P Presenting author

P246

New approach methodologies (NAMs) as first tier in an integrated approach to testing and assessment for non-genotoxic carcinogens

Manon Bouwmeester^P1, André  Muller², Barira Islam³, Tjalf de Boer⁴, Clémence  Budin⁴, Emiel Rorije², Mirjam Luijten^1
1 Centre for Health Protection, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands
² Centre for Safety of Substances and Products, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands
³ Certara Predictive Technologies, Certara UK Limited, Sheffield, S1 2BJ, United Kingdom
⁴ BioDetection Systems BV, Science Park 406, 1098 XH, Amsterdam, The Netherlands
^P Presenting author

P247

Estimation of intra-specific ranges of genetic susceptibility to toxicity using genetically diverse populations of human lymphoblastoid cell lines.

Konrad Patyra¹, Maria Adonay^2,3, Robert Anholt^2,3, Trudy Mackay^2,3, Pekka Kohonen^1,4, John  Colbourne⁵, Juha Rantala¹, Roland Grafström^1,4, Vesa Hongisto^P1
1 Misvik Biology Oy, Turku, Finland
² Center for Human Genetics, Clemson University, Greenwood, SC, USA
³ Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA
⁴ Institute for Environmental Medicine, Karolinska Institutet, Solna, Sweden
⁵ School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, United Kingdom
^P Presenting author

P248

High-throughput RNA sequencing reveals distinct transcriptomic responses of human jurkat T cells to cyanotoxins and their binary mixture

Ronald F Borja¹, Antonio Casas-Rodríguez¹, Mónica Pérez-Alegre², Eloisa Andújar², María Puerto¹, Angeles Jos^1
1 Area of Toxicology, Faculty of Pharmacy, University of Seville, Seville, Spain.
² Genomics Unit, Andalusian Centre of Molecular Biology and Regenerative Medicine (CABIMER), CSIC, University of Seville,  Pablo de Olavide University, Seville, Spain
^P Presenting author

P249

Evaluation of the neurotoxicity of biocide isothiazolinone

Ha-Na Oh¹, Woo-Keun Kim^P1
1 Korea Institue of Toxicology
^P Presenting author

P250

Human relevance assessment of adverse outcome pathways and associated NAMs

Annick van den Brand^P1, Elisavet Renieri², Emma Di Consiglio³, Graciela López Soop⁴, Jeske van Boxel¹, Mirjam Luijten^1
1 Rijksinstituut voor Volksgezondheid en Milieu (RIVM) – NL
² Aristotle University of Thessaloniki (AUTH) – GR
³ Istituto Superiore di Sanità (ISS) – IT
⁴ National Institute of Occupational Health (STAMI) – NO
^P Presenting author

P252

Transcriptomic–based analysis to discriminate non-genotoxic carcinogens from genotoxic carcinogens and non-carcinogens in a human HepaRG spheroid model: mechanistic insights and biomarker identification

Mariam Saleh^P1, Kevin Hogeveen¹, Thibaut  Leger¹, Valérie Fessard¹, Ludovic Le Hégarat^1
1 Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail (ANSES)
^P Presenting author

P253

Characterization of the performance and sensitivity of deiodinase 1 inhibition bioassay using different in vitro models

Tereza Nešporová¹, Veronika Janečková¹, Marie Smutná¹, Jiri Novák^1
1 Masaryk University
^P Presenting author

P254

Establishment and optimization of thawing procedures for murine non-parenchymal liver cells as progress of primary hepatic co-culture systems

Emina Wagner^P1, Tanja Krimmling¹, Anett Ullrich^1
1 PRIMACYT Cell Culture Technology GmbH
^P Presenting author

P255

Metabolic activities in rainbow trout (oncorhynchus mykiss) S9 fractions liver and extrahepatic organs as an alternative in vitro ecotoxicity assessment approach

Marcel Reu¹, Tanja Krimmling^P1, Melissa Thiede¹, Amani Alkufairi¹, Alexander Sattler¹, Jennifer Schuldt¹, Dieter  Runge¹, Anett Ullrich^1
1 PRIMACYT Cell Culture Technology GmbH
^P Presenting author

P256

Impact of microplastics in ambient air on A549 alveolar cells and RPMI 2650 nasal cells

Camille BONNEFOUS^P1,2, Frédéric LEDOUX^1,2, Yann LANDKOCZ^1,2
1 UCEiV UR4492 – Dunkerque – France
² Université du Littoral Côte d’Opale – Dunkerque – France
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P257

Perimyelintox: developing a peripheral demyelination assay

Cody Niedergeses^P1, Lisa Maria Haiber¹, Britta Anna Kühne^1,2, Julia Spänle¹, Peter Schertl³, Bettina Seeger^1
1 Institute for Food Quality and Safety, Research Group Food Toxicology and Alternative/Complementary Methods to Animal Experiments, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany
² German Federal Institute for Risk Assessment (BfR), German Centre for the Protection of Laboratory Animals (BfR), Berlin, Germany
³ Institute of Cell Biology and Biophysics, Research Group Cell Biology, Leibniz University Hannover, 30419 Hannover, Germany 
^P Presenting author

P258

Potency-response (PR) plots: a new tool for the visualization of complex data sets

Yusuf Hussien¹, Mathyn Rijkers¹, Giel Hendriks^P1, Inger Brandsma^1
1 Toxys
^P Presenting author

P259

Determination of the genotoxic and chemopreventive properties of beta-sitosterol on human peripheral lymphocytes using the comet assay

Elif Aktaş^P1, Deniz  Yüzbaşıoğlu², Ebru Beyzi³, Fatma  Ünal^2
1 Gazi University, Graduate School of Natural and Applied Sciences, Ankara, Türkiye
² Gazi University, Faculty of Science, Biology Department, Ankara, Türkiye
³ Gazi University, Vocational School of Health Services, Department of Medical Services and Techniques, Ankara, Türkiye
^P Presenting author

P260

Standardizing and automating high throughput organoid culture and downstream applications with the mo:re lab platform

Julia Vallverdu¹, Daniel Ziemianowicz ¹, Dana Hellmold^1
1 mo:re GmbH
^P Presenting author

P261

Comparative evaluation of in silico and in vitro tools for the prediction of metabolites in livestock studies

Kristin Diercks^P1, Marc Lamshoeft¹, Mark-Christoph  Ott^1
1 Bayer AG
^P Presenting author

P262

Comparing natural toxins and pesticides: A broad targeted metabolomics analysis in HepaRG cells

Sahra Kiessig^P1, Philipp Ternes², Nicole Rocker², Philip Marx-Stoelting¹, Daniela Morais Leme³, Hennicke Kamp^2
1 Pesticides Safety, German Federal Institute for Risk Assessment
² BASF Metabolome Solutions GmbH
³ Department of Genetics, Federal University of Paraná (UFPR)
^P Presenting author

P263

Drosophila melanogaster as a model to investigate the effects of environmental contaminants on the gastrointestinal microbiota.

Alba García-Rodríguez^P1, Arnau Rocabert¹, Raquel Egea¹, Ricard Marcos¹, Alba Hernández^1
1 Universitat Autònoma de Barcelona
^P Presenting author

P264

Optimization of exogenous metabolic activation system for enhanced detection of nitrosamines using MultiFlow and MicroFlow assays

Steven Bryce^P1, Svetlana Avlasevich¹, Jeff  Bemis¹, Stephen Dertinger^1
1 Litron Labs
^P Presenting author

P265

Refining gold standard in vitro models for the NAMs era

Maria Karlgren^P1
1 Department of Pharmacy, Uppsala University, Sweden
^P Presenting author

P266

In vitro genotoxicity – investigation of different mechanisms using micronucleus test via flow cytometry

Marla Lindemann^P1, Elisabeth Muschiol¹, Louisa Tölke¹, Thorsten Kuczius², Melanie Esselen^1
1 Institute of food chemistry, University of Münster, Corrensstraße 45, 48149 Münster, Germany
² Institute of Hygiene, University Hospital Münster, Robert Koch-Straße 41, 48149 Münster, Germany
^P Presenting author

P267

From insights to impact: acceleration of NAM implementation by policymakers

Jeske van Boxel^P1, Vera Versteegh², Tim  Verbruggen¹, Eva Streekstra¹, Victoria  de Leeuw¹, Jelle  Vriend¹, Anne  Kienhuis^1,3
1 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands
² Utrecht University, Faculty of Medicine, Utrecht, the Netherlands
³ Institute for Risk Assessment Sciences (IRAS), Utrecht University, Utrecht, the Netherlands
^P Presenting author

P268

The concise summary of the country reports of the Asian federation of societies for alternatives to animal experiments (AFSAAE)

Atsushi Miyashita^P1,2, D.P.N. De Silva^3,4, Vijay Pal Singh^5,6, Tae-Eun Park^7,8, Yuan Pang^9,10
1 Institute of Medical Mycology, Teikyo University, Japan
² Japanese Society for Alternative to Animal Experiments
³ Department of Chemistry, University of Colombo, Sri Lanka
⁴ Society for Alternatives to Animal Testing in Sri Lanka
⁵ CSIR- Institute of Genomics and Integrative Biology, India
⁶ Society for Alternatives to Animal Experiments (SAAE-India)
⁷ Department of Biomedical Engineering, UNIST, Republic of Korea
⁸ Korean Society for Alternatives to Animal Experiments
⁹ Department of Mechanical Engineering, Tsinghua University, China
¹⁰ Society of Toxicological Alternatives and Translational Toxicology
^P Presenting author

P269

In vitro safety assessment of locally produced biosimilars and herbal products in Pakistan using 3D liver and kidney microstructures

Jibran Khan^P1
1 Jinnah University for Women
^P Presenting author

P270

Evaluation of surface water quality with biochemical assays

Francois Gagne^P1, Chantale André^1
1 Environment and Climate Change Canada
^P Presenting author

P271

Standardizing sample preparation and delivered dose in complex in vitro models

Marina  Pérez Gregorio^P1, Amira Fernández¹, Juan Francisco Ferrer¹, Cristina  Furió ¹, María  Lorenzo¹, Paula Smudde^2
1 AIMPLAS (Plastics Technology Centre), Valencia
² Universitat Politécnica de Valencia (UPV)
^P Presenting author

P272

A decision tree framework to restrict animal testing as a last resort: A case example for acute oral toxicity assessment in the European union

Giorgia Pallocca^P1, Patricia  Bishop¹, Patience  Browne², Marie  Darracq-Ghitalla-Ciock³, Fabian  Grimm⁴, Laura  Holden⁵, Jay  Ingram¹, Kamel  Mansouri⁶, Hans  Raabe⁷, Clive  Roper⁸, Katherine  Santizo⁹, Barbara G.  Schmitt¹⁰, Thomas A  Ward¹¹, Catherine  Willett^1
1 Humane World for Animals
² Organization for Economic Cooperation and Development (OECD)
³ KREATiS
⁴ Clariant Produkte GmbH
⁵ University of Birmingham
⁶ NICEATM/NIEHS/NIH
⁷ Institute for In Vitro Sciences
⁸ Roper Toxicology Consulting Limited
⁹ CEFIC
¹⁰ Evonik Operations GmbH
¹¹ XCellR8
^P Presenting author

P273

Refining the adverse outcome pathway (AOP) for radon-induced lung cancer: integrating genetic variability and environmental co-exposures to improve risk characterization

Klea Hondro^P1, Paul  Locke^2
1 myself
² Professor/Mentor
^P Presenting author

P274

Miniaturized Ames test systems for nitrosamine mutagenicity screening: A systematic evaluation of miniaturized Ames test formats as sustainable alternatives to conventional genotoxicity testing

Csaba Boglári^P1, Cécile  Koelbert^1
1 Xenometrix AG
^P Presenting author

P275

In vitro wound healing model for evaluation of cutaneous re-epithelization

Marek Puskar^P1, Michael Bachelor², Jonathan Oldach², Gina Stolper², Max Li², Alex Armento², Patrick Hayden², Silvia Letasiova^1
1 MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
² MatTek Corporation, Ashland, MA, USA
^P Presenting author

P276

Male reproductive toxicity induced by aminated polystyrene microplastics

Mahmoud Abudayyak^P1, Özge Sultan Zengin², Gül Özhan¹, Mohamed F. Abdallah^3
1 Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Türkiye.
² Institute of Graduate Studies in Health Sciences, Istanbul University, Türkiye.
³ Department of Food Science, Aarhus University, Denmark.
^P Presenting author

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Regulatory considerations for complex NAMs

P277

Roadmap on phasing out animal testing for chemical safety assessments

Katrin Schutte^P1
1 European Commission
^P Presenting author

P278

Mechanistically informed in vitro genotoxicity model for complex molecules: NAMs to prevent unnecessary in vivo follow-up tests in regulatory field

Giulia Martina Sitzia^P1, Monica Locatelli^1
1 Kahlberg Consulting
^P Presenting author

P279

Distinguishing adult and developmental neurotoxicity using new approach methodologies: NAM4NT

Jonathan Blum^P1,2, Tamara Tal^3,4, Ellen Fritsche^1,2
1 Swiss Centre for Applied Human Toxicology – SCAHT, Switzerland
² Department of Pharmaceutical Sciences, University of Basel, Switzerland
³ Department of Ecotoxicology, Helmholtz-Centre for Environmental Research – UFZ, Leipzig, Germany
⁴ Medical Faculty, University of Leipzig, Leipzig, Germany
^P Presenting author

P280

A whole-mixture prioritisation approach of plant protection products for non-dietary risk assessment using NAMs

Alkiviadis Stagkos-Georgiadis^P1,2, Philip Marx-Stoelting¹, Tewes Tralau³, Denise Bloch^1
1 Department of Pesticides Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, Germany
² University of Potsdam, Institute of Nutritional Science, Department of Nutritional Toxicology, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany
³ German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Str. 8-10, 10589 Berlin, Germany
^P Presenting author

P281

Establishing an adverse outcome pathway for developmental neurotoxicity: inhibition of voltage-gated sodium channels leading to impaired myelination and cognitive dysfunction

Lidwina Gerner^P1,2, Jonathan Blum^1,2, Ellen Fritsche^1,2,3
1 SCAHT – Swiss Centre for Applied Human Toxicology, Basel, Switzerland
² Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland
³ DNTOX GmbH, Düsseldorf, Germany
^P Presenting author

P282

Standardization of hiPSC-derived intestinal epithelial cell models

Loes PM Duivenvoorde¹, Deborah  Rijkers ¹, Meike van der Zande¹, Aafke WF Janssen^P1
1 Wageningen Food Safety Research, part of Wageningen University & Research, Wageningen, The Netherlands
^P Presenting author

P283

Global harmonization and modernization of safety pharmacology to support the adoption of emerging technologies

Jan Turner^P1, Colleen Pike¹, Jennifer  Pierson², Claire O’Brien², Alexandra  Taraboletti², Yasunari Kanda³, Sonja  Beken⁴, Derek Leishman⁵, Jean-Pierre Valentin^6
1 Humane World for Animals, Brussels, Belgium
² Health and Environmental Sciences Institute (HESI), Washington, D.C., United States of America
³ National Institute of Health Sciences (NIHS), Kawasaki, Kanagawa, Japan
⁴ Belgian Federal Agency for Medicines and Health Products (FAMHP), Brussels, Belgium
⁵ Eli Lilly and Company, Indianapolis, Indiana, United States of America
⁶ UCB Biopharma (UCB S.A.), Brussels, Belgium
^P Presenting author

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Role of 3R centres in the dissemination and implementation of NAMs

P284

Advancing toxicology through microphysiological systems: the 3Rs collaborative’s efforts to drive adoption and regulatory confidence

Megan LaFollette¹, Emily Richardson², Clive Roper^P3,1
1 The 3Rs Collaborative
² CN Bio Innovations
³ Roper Toxicology Consulting Limited
^P Presenting author

P285

Centro 3R: advancing 3Rs principles in education, research, and beyond

Valeria Chiono^1,2
1 Politecnico di Torino
² Centro 3R
^P Presenting author

P286

A novel approach for constructing adverse outcome pathways using transcriptomics-based dual-evidence key event identification

Jin Yoo^P1,2, Yubin Han³, Ji Yeong Park^1,2, Jung Eun Lim¹, Ga Eun Kim¹, In Jae Bang³, Haryong Kim^1,2
1 College of Pharmacy, Korea University, Republic of Korea
² Interdisciplinary Major Program in Innovative Pharmaceutical Sciences, Korea University, Republic of Korea
³ Institute of Pharmaceutical Science and Translational Research, Korea University, Republic of Korea
^P Presenting author

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Serum-free approaches

P287

Revisiting 3Rs: rethinking replacement and new approach methodologies

Jessica  Rosolowski ¹, Tilo Weber^P1, Atena Malakpour-Permlid², Stina Oredsson^3
1 Animal Welfare Academy of the German Animal Welfare Federation, 85579 Neubiberg, Germany
² Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
³ Department of Biology, Lund University, 223 62 Lund, Sweden
^P Presenting author

P288

Advancing in vitro cell culture practices: achieving truly animal-free experiments and scientifically reliable and reproducible methods

Stina Oredsson¹, Tilo Weber^P2, Atena Malakpour-Permlid^3
1 Department of Biology, Lund University, 223 62 Lund, Sweden
² Animal Welfare Academy of the German Animal Welfare Federation, 85579 Neubiberg, Germany
³ Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
^P Presenting author

P289

Self-assembling peptide hydrogels as user-defined synthetic matrices for in vitro modelling

Clive Roper^P1, John Curd¹, Viola Erdelyi¹, Mikayla Shelton¹, Cathy Merry^1
1 PeptiMatrix Limited
^P Presenting author

P290

A cost-effective animal-free approach for generating primary human hepatocyte spheroids

Rowena Sison-Young^P1,2, Chris  Goldring^1,2, Ian Copple^1,2
1 Human Liver Research Facility, Liverpool Shared Research Facilities, Faculty of Health and Life Sciences, University of Liverpool, United Kingdom L69 3GE
² Department of Pharmacology and Therapeutics, Faculty of Health and Life Sciences, University of Liverpool, United Kingdom L69 3GE
^P Presenting author

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Toxicokinetics and in vitro – in vivo extrapolation

P291

In vitro study of the biotransformation of cylindrospermopsin using human and RAT liver microsomes

Antonio Cascajosa-Lira¹, Remedios Guzmán-Guillén^P1, Silvia Pichardo¹, Ana María Cameán¹, Angeles Jos¹, Ana I. Prieto^1
1 Toxicology Area, Faculty of Pharmacy, University of Seville, Spain.
^P Presenting author

P292

Two-layer percutaneous absorption integrated in a PBK framework improves plasma concentration prediction for NGRA of cosmetic ingredients

Kazutoshi Saito^P1
1 Kao corporation
^P Presenting author

P293

Working towards the implementation and acceptance of QIVIVE in NAM-based risk assessment

Anne Zwartsen^P1, Véronique  de Bruijn¹, Joost Westerhout¹, Victoria de Leeuw¹, Jordi Minnema¹, Thijs Benschop¹, Lianne de Wit-Bos¹, Eva  Streekstra¹, Suzanne Jeurissen¹, Bas Bokkers^1
1 National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands
^P Presenting author

P294

In vitro-in vivo response-response relationships for the in vitro based risk assessment of chemical substances

Thijs Benschop^P1, Jordi Minnema¹, Emma Kasteel², Anne Zwartsen¹, Jelle Vriend¹, Anne Kienhuis^1,2, Remco Westerink², Agnes Oomen^1,3, Bas Bokkers^1
1 National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands
² Institute for Risk Assessment Sciences (IRAS), Utrecht University, Utrecht, The Netherlands
³ University of Amsterdam (UvA), Amsterdam, The Netherlands
^P Presenting author

P295

Evaluating acrylamide developmental neurotoxicty using the adverse outcome pathway strategy

Roseline Ayowumi Awoga^P1, Birgitte Lindeman ², Marcin W.  Wojewodzic^2,3, Arup Ghosh², Vajagathali Mohammed¹,  Ylva  Johansson ¹, Jan Alexander², Oddvar Myhre², Anna Forsby^1
1 Department of Biochemistry and Biophysics, Stockholm University, Sweden
² Division of Climate and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway
³ Cancer Registry, Norwegian Institute of Public Health, Oslo, Norway
^P Presenting author

P296

NAM-based multi-omics workflow in zebrafish embryo toxicology: A pilot study with 3,4-dichloroaniline

Andrea Weiner¹, Mark R. Viant², Elena Sostare², Mari Paz Gallego¹, Idoia Ibarburu¹, Lauren Cruchley-Fuge³, Ossama Ebdali³, Andrew Southam³, Ralf Weber³, John K Coulbourne², Arantza Muriana^P1
1 BBD BioPhenix SL – Biobide
² Michabo Health Science Ltd., UK
³ University of Birmingham
^P Presenting author

P297

Insight: an in silico tool to support chemical fate prediction and NAM-based assay design

Pierre-Andre Billat^P1, Daniela  Brenner², Kévin Bernal³, Eliška  Sychrová², Jiří  Kohoutek², Sophie  Langouet⁴, Rayane  Boufalaas^1,5, Julien  Dairou⁶, Arnaud  Tête³, Pavel  Babica², Etienne  Blanc³, Iva Sovadinová², Cleo Tebby⁷, Remy  Beaudouin^8,9
1 INERIS, Experimental toxicology and modeling unit (TEAM), Parc ALATA BP2, Verneuil en Halatte, France
² RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic
³ Université Paris Cité, INSERM, Health & Functional Exposomics – HealthFex, 75006 Paris, France
⁴ Université Rennes, INSERM, EHESP, Irset (Institut de recherche en santé, environnement et travail) – UMR_S 1085, 35000 Rennes, France.
⁵ University of technology of Compiegne, CNRS, Biomechanics and Bioengineering, Centre of recherche Royallieu- CS 60319, 60203 Compiegne Cedex, France.
⁶ Université Paris Cité, CNRS, Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, F-75006 Paris, France. 45 rue des Saints Pères, 75006 Paris, France
⁷ INERIS, Experimental toxicology and modeling unit (TEAM), Parc ALATA BP2, Verneuil en Halatte, France
⁸  INERIS, Experimental toxicology and modeling unit (TEAM), Parc ALATA BP2, Verneuil en Halatte, France
⁹ UMR-I 02 SEBIO, INERIS, Parc ALATA BP2, Verneuil en Halatte, France
^P Presenting author

P298

Evaluating toxicokinetics in mixture toxicity: identifying potential CYP inhibitors and substrates to establish common kinetic groups (CKG)

Asya Kadic^P1,2, Bjoern  Liebnau^1,3, Albert Braeuning¹, Denise Bloch¹, Philip Marx-Stoelting^1
1 German Federal Risk Assessment Institute
² Technical University of Dortmund
³ Potsdam University
^P Presenting author

P299

Transport and toxicity of indoxyl sulfate and p-cresyl sulfate across physiological barriers

Pierre Spicher^P1,2, Lucie Dehouck², Marie-Christine Boucau², Maxime Culot², Sandra Bodeau^1,3
1 MP3CV Laboratory, UR7517, Jules Verne University of Picardie, F-80000 Amiens, France
² Blood-Brain Barrier Laboratory (LBHE), UR 2465, University of Artois, F-62300 Lens, France
³ Department of Clinical Pharmacology, Amiens University Medical Center, F-80000 Amiens, France
^P Presenting author

P300

Mass balance is not related to pesticide physicochemical properties and in vitro model suitability for absorption studies

Alex KleinJan^P1, Kitty  van Summeren¹, Jean Paul  ten Klooster¹, Emily  Rayner², Evita  van de Steeg², Yvonne  Staal³, Raymond  Pieters^1,4, Cyrille  Krul^1
1 Innovative Testing in Life Sciences and Chemistry, Research Centre Healthy and Sustainable Living, University of Applied Sciences Utrecht, Utrecht, the Netherlands
² Department of Metabolic Health Research, Netherlands Organisation for Applied Scientific Research (TNO), 2333 BE Leiden, The Netherlands
³ National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
⁴ Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands
^P Presenting author

P301

Next generation risk assessment: an integrated phenotypic profiling and IVIVE approach for plant protection product mixture effects

Yemurai Musengi^P1
1 German Federal Institute for Risk Assessment (BfR)
^P Presenting author

P302

Physiologically-based kinetic (PBK) modelling dataset: an open resource to increase accessibility of PBK models for safety assessment

Alessio Gamba^P1, Peter Penson¹, Philippa McCabe¹, Judith Madden^1
1 School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, UK
^P Presenting author

P303

In vivo toxicokinetic mechanisms of new psychoactive substances: A narrative review

Hala Harifi^P1
1 Laboratory of Biology and Health, Faculty of Science, Ibn Tofail University, Kenitra, Morocco
^P Presenting author

P304

Cytotoxicity of tea polyphenols and their complex phase II metabolism

Johannes Gröne^P1, Julian Alfke¹, Erik Reichel¹, Svenja  Wälscher¹, Melanie Esselen^1
1 Institute of Food Chemistry, University of Münster, Corrensstraße 45, 48149, Münster, Germany
^P Presenting author

P305

Integration of in vitro toxicity and high-throughput PBK modelling enables prospective prediction of drug-induced liver injury

René Geci^P1, Ahenk Zeynep Sayin¹, Stephan Schaller², Lars Kuepfer^1
1 University Hospital RWTH Aachen
² ESQlabs GmbH
^P Presenting author

P306

High-throughput PBK modelling for systemic PK predictions after dermal exposure

Zeynep Edizcan¹, Stephan Schaller², Lars Kuepfer¹, René Geci^P1
1 University Hospital RWTH Aachen
² ESQlabs GmbH
^P Presenting author

P307

Complex distribution phenomenon of test chemicals in cell culture experiments: exemplification by tebufenpyrad

Mahshid Alimohammadi^P1, Hiba  Khalid², Elias Zgheib², Anna-Katharina  Holzer¹, Naja  Bürgers¹, Viktoria  Magel¹, Marcel  Leist^1,3, Baiba  Gukalova⁴, Edgars  Liepinsh^4
1 In vitro Toxicology and Biomedicine, University of Konstanz, 78457 Konstanz, Germany
² Certara Predictive Technologies, Level 2-Acero, 1 Concourse Way, Sheffield S1 2BJ, UK
³ Center for Alternatives to Animal Testing in Europe (CAAT-Europe), University of Konstanz, 78457 Konstanz, Germany
⁴ Latvian Institute of Organic Synthesis, LV-1006 Riga, Latvia
^P Presenting author

P308

Non-human primate gut organoids: A translational in vitro platform for gastrointestinal drug safety testing

Laura Rijns^P1, Fabian P. Suchy^1,2, Lucia  Ramirez¹, Morteza Roodgar^1,2
1 Exir Bio, Menlo Park, CA 94025, USA.
² Stanford University, School of Medicine, Stanford University, Stanford, CA 94305, USA.
^P Presenting author

P309

Human‑relevant in vitro approaches to assess gastrointestinal toxicity of food‑grade titanium dioxide (E171)

Simone G. van Breda^P1, Nicolaj S. Bischoff¹, Dick T. Sijm², Theo M. de Kok^1
1 Department of Translational Genomics, Maastricht University, Maastricht, The Netherlands
² Department of Pharmacology & Toxicology, Maastricht University, Maastricht, The Netherlands
^P Presenting author

P310

Impact of dose metric selection on transcriptomic point of departure (tPOD) derivation in in vitro toxicology

Richard Currie¹, Sevil Payvandi¹, Yeyejide Adeleye¹, Jack Feltham¹, Kathryn Rudd^P1
1 Syngenta, Bracknell, Berkshire, United Kingdom
^P Presenting author

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