Scientific papers and book chapters

European Society of Toxicology In Vitro > Scientific papers and book chapters

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2025

Grosso MF, Řehůřková E, Virmani I, Sychrová E, Sovadinová I, Babica P. Impact of Endocrine Disruptors on Key Events of Hepatic Steatosis in HepG2 Cells. Food Chem Toxicol. 2025;115241. https://doi.org/10.1016/j.fct.2025.115241

Burla S, Chary A, Serchi T, Cambier S, Sullivan K, Baker E, Sadekar N, Gutleb AC. Responses of an In Vitro Coculture Alveolar Model for the Prediction of Respiratory Sensitizers (ALIsens^®) Following Exposure to Skin Sensitizers and Non-Sensitizers. Toxics. 2025; 13(1):29. https://doi.org/10.3390/toxics13010029

LaFollette MR, Baran SW, Curley JL, Dickinson AM, Frazier T, Hobi N, Huang MI, Hutter V, Maisonneuve BGC, Marsh GA, Mahendran R, Müller I, Qian X, Singh D, Thelin WR, Vukasinovic J, Candarlioglu PL, Roper CS. The Use of MPS in Three Rs and Regulatory Applications: Perspectives From Developers on Stakeholder Responsibilities. Altern Lab Anim. 2025 Jan;53(1):26-41. doi: 10.1177/02611929241310566. PMID: 39772941

Seifert S, Siewert K, Curato C, Sonnenburg A, Fritsch N, Peiser M, Willenbockel CT, Martin S, Bloch D. Using new approach methodologies for the identification of a sensitizing co-formulant in a plant protection product. Toxicology. 2025 Feb 28;514:154100. doi: 10.1016/j.tox.2025.154100

2024

ALTEX. 2024 Mar 19. Online ahead of print. The benefits of validation of methods for toxicity testing outweigh its costs Anne Gourmelon, Philippe Hubert , Elise Grignard , Lisa Baumann, Sharon Munn , Cécile Michel. doi: 10.14573/altex.2403051.

Chowdhury RR, Grosso MF, Gadara DC, Spáčil Z, Vidová V, Sovadinová I, Babica P. Cyanotoxin cylindrospermopsin disrupts lipid homeostasis and metabolism in a 3D in vitro model of the human liver. Chem Biol Interact. 2024;397:111046. https://doi.org/10.1016/j.cbi.2024.111046

Chowdhury RR, Rose S, Ezan F, Sovadinová I, Babica P, Langouët S. Hepatotoxicity of cyanotoxin microcystin-LR in human: Insights into mechanisms of action in the 3D culture model Hepoid-HepaRG. Environ Pollut. 2024;342:123047. https://doi.org/10.1016/j.envpol.2023.123047

Raptová P, Skočková V, Babica P, Sovadinová I, Sychrová E, Vídeňská P, Šplíchalová P, Vašíček O, Šindlerová L. Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro. Environ Toxicol Pharmacol. 2024;105:104342. https://doi.org/10.1016/j.etap.2023.104342

Skočková V, Raptová P, Pospíchalová K, Sovadinová I, Sychrová E, Smutná M, Hilscherová K, Babica P, Šindlerová L. Cyanobacterial harmful bloom lipopolysaccharides: pro-inflammatory effects on epithelial and immune cells in vitro. Arch Toxicol. 2024;98:481-491. https://doi.org/10.1007/s00204-023-03644-8

Bridgeman, L.; Juan, C.; Berrada, H.; Juan-García, A. 2024. Effect of acrylamide and mycotoxins in SH-SY5Y cells: A Review. Toxins 16, 87. https://doi.org/10.3390/toxins16020087

Bovio F, Perciballi E, Melchioretto P, Ferrari D, Forcella M, Fusi P, Urani, C. Morphological and metabolic changes in microglia exposed to cadmium: Cues on neurotoxic mechanisms. Environ Res. 2024 240(Part 1 (1 January 2024) 10.1016/j.envres.2023.117470

Brackin T, Gayes H, Johnson I, Fox D, and Roper C. (2024). Skin barrier function for regulatory skin absorption tests and effects on testosterone and sucrose absorption. Toxicology in Vitro 95; 105735. https://doi.org/10.1016/j.tiv.2023.105735

Halinkovč M, Mušková K, Sloboda T, Lepáček M, Kandarova H., Ries M, Šoltesová-Prnová M (2024). MLtox, online phototoxicity prediction webpage. In Toxicology in vitro: the official journal of the European Society for Toxicology in Vitro, 2024, vol. 94, art. no. 105701. ISSN 0887-2333. https://doi.org/10.1016/j.tiv.2023.105701

Hartung T., Morales Pantoja IE and Smirnova L. Brain organoids and Organoid Intelligence (OI) from ethical, legal, and social points of view. Frontiers in Artificial Intelligence, sec. Organoid Intelligence 2024, 6:1307613. https://doi.org/10.3389/frai.2023.1307613

Hartung T. Evidence-based toxicology. Encyclopedia of Toxicology, 4^th edition, in press. DOI:10.1016/B978-0-12-824315-2.00973-8

Hartung T., Toxicity Testing in the 21st Century: Approaches to Implementation. Encyclopedia of Toxicology, 4^th edition, in press. DOI:10.1016/B978-0-12-824315-2.00979-9

Hartung T. The (misleading) role of animal models in drug development. Frontiers in Drug Discovery 2024, in press. doi: 10.3389/fddsv.2024.13550442

Maertens A, Luechtefeld T, Knight J and Hartung T. Alternative Methods Go Green! Green Toxicology as a Sustainable Approach for Assessing Chemical Safety and Designing Safer Chemicals. ALTEX, in press. https://doi.org/10.14573/altex.2312291

Alam El Din D-M, Shin J, Lysinger A, Roos MJ, Johnson EC, Shafer TJ, Hartung T and Smirnova L. Organoid Intelligence for Developmental Neurotoxicity Testing. Front. Cell. Neurosci. – Cellular Neurophysiology 2024, 18:1480845. https://doi.org/10.3389/fncel.2024.1480845

Balls M, Bass R, Curren R, Fentem J, Goldber A, Hartung T, Herrmann K, Kleinstreuer NC, Libowitz L, Parascandola J, Rowan A, Spielmann H, Stephens ML, Thomas RS and Tsaioun K. 60 Years of the 3Rs Symposium: Lessons Learned and the Road Ahead. ALTEX 2024, 41:179-201. doi: 10.14573/altex.2403061.

Bhuller Y, Hilton GM, Avey M, Marles RJ, Trombetti S, Hartung T, Caloni F, Cazzaniga A, Gutleb AC, Hartung T, Kandarova H, Ranaldi G, Rashidi H, Wilflingseder D and Moutaharrik S. Non Animal Models: complexity for interactions……connecting Science. The Fifth Virtual Summer School Lake Como School of Advanced Studies, 15-16 May 2024, ALTEX 2024, 41:666-668. doi: 10.14573/altex.2407181

Cauchemez S, Cossu G, Delzenne N, Elinav E, Fassin D, Fischer A, Hartung T, Kalra D, Netea M, Neyts J, Rappuoli R, Pizza M, Saville M, Tenaerts P, Wright G, Sansonetti P, and Goldman M. Standing the test of COVID-19: charting the new frontiers of medicine. Frontiers in Science 2024, http://dx.doi.org/10.3389/fsci.2024.1236919

Debad S, Allen D, Bandele O, Bishop C, Blaylock M, Brown P, Bunger MK, Co JY, Crosby L, Daniel AB, Ferguson SS, Ford K, Gamboa da Costa G, Gilchrist KH, Grogg MW, Gwinn M, Hartung T, Hogan SP, Jeong YE, Kass GEN, Kenyon E, Kleinstreuer NC, Kujala V, Lundquist P, Matheson J, McCullough SD, Melton-Celsa A, Musser S, Oh I, Oyetade OB, Patil SU, Petersen EJ, Sadrieh N, Sayes CM, Scruggs BS, Tan Y-M, Thelin B, Nelson MT, Tarazona JV, Wambaugh JF, Yang J-Y, Yu C and Fitzpatrick S. Trust your gut: Establishing confidence in gastrointestinal models – An overview of the state of the science and contexts of use. ALTEX 2024, 41:402-24. Doi: 10.14573/altex.2403261.

Diemar M, Vinken M, Teunis M, Krul C, Busquet F, Zajac J, Kandarova H, Corvi R, Russo M, Kharina A, Bryndum L, Santillo M, Bloch D, Kucheryavenko O, Panagiotakos D, Rogiers V, Beekhuijzen M, Giusti A, Najjar A, Courage C, Koenig T, Kolle S, Boonen H, Dhalluin S, Boberg J, Müller B, Kukic P, Ritskes-Hoitinga M, Grasselli E, Zietek T, Stoddart G, Heusinkveld H, Castell J, Benfenati E, Yang H, Perera S, Paini A, Kramer N, Hartung T, Janssen M, Fritsche E, Jennen D, Piumatti M, Rathman J, Marusczyk J, Milec L, and Roggen E. Report of the first ONTOX Stakeholder Network meeting: digging under the surface of ONTOX together with the stakeholders. ATLA, in press. Doi: 10.1177/02611929231225730.

Balogh A, Bódi-Jakus M, Karl VR, Bellák T, Széky B, Farkas J, Lamberto F, Novak D, Fehér A, Zana M, Dinnyés A. Establishment of human pluripotent stem cell-derived cortical neurosphere model to study pathomechanisms and chemical toxicity in Kleefstra syndrome. Sci Rep. 2024;14:22572. https://doi.org/10.1038/s41598-024-72791-4

Kistamás K, Lamberto F, Vaiciuleviciute R, Leal F, Muenthaisong S, Marte L, Subías-Beltrán P, Alaburda A, Arvanitis DN, Zana M, Costa PF, Bernotiene E, Bergaud C, Dinnyés A. The Current State of Realistic Heart Models for Disease Modelling and Cardiotoxicity. Int J Mol Sci. 2024;25. https://doi.org/10.3390/ijms25179186

Chandrasekaran V, Wellens S, Bourguignon A, Djidrovski I, Fransen L, Ghosh S, Mazidi Z, Murphy C, Nunes C, Singh P, Zana M, Armstrong L, Dinnyés A, Grillari J, Grillari-Voglauer R, Leonard MO, Verfaillie C, Wilmes A, Zurich MG, Exner T, Jennings P, Culot M. Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures. Toxicology in Vitro. 2024;98. https://doi.org/10.1016/j.tiv.2024.105826

Fink F, Hartung T, Lee SY and Maynard A. AI for scientific discovery pioneering new frontiers in knowledge. In: World Economic Forum, Top 10 Emerging Technologies of 2024, Flagship Report. Available at: https://www.weforum.org/publications/top-10-emerging-technologies-2024/in-full/1-ai-for-scientific-discovery/

Gao Y, Mughal Z, Jaramillo-Villegas JA, Corradi M, Borrel A, Lieberman B, Sharif S, Shaffer J, Fecho K, Chatrath A, Maertens A, Teunis MAT, Kleinstreuer N, Hartung T, and Luechtefeld T. BioBricks.ai: A Versioned Data Registry for Life Sciences Data Assets. https://arxiv.org/abs/2408.17320

Golden E, Allen D, Amberg A, Anger LT, Baker E, Baran SW, Bringezu F, Clark M, Duchateau-Nguyen G, Escher SE, Giri V, Grevot A, Hartung T, Li D, Muster W, Snyder K, Wange R and Steger-Hartmann T. Toward implementing virtual control groups in nonclinical safety studies: Workshop report and roadmap to implementation. ALTEX, 41:282–301. doi: 10.14573/altex.2310041.

Hansell L, Ritskes-Hoitinga M, Visseren-Hamakers IJ and Hartung T. Recommendations for the EU roadmap to accelerate the transition towards phasing out animal testing for chemical safety assessments. Frontiers Policy Lab 2024, available at: https://policylabs.frontiersin.org/content/commentary-recommendations-for-the-eu-roadmap-to-accelerate-the-transition-towards-phasing-out-animal-testing-for-chemical-safety-assessments

Hartung T, The validation of regulatory test methods – conceptual, ethical, and philosophical foundations. ALTEX 2024, 41:525–544. doi: 10.14573/altex.2409271.

Hartung T, King N, Kleinstreuer N, Leist M and Tagle D. Leveraging Biomarkers and Translational Medicine for Preclinical Safety – Lessons for Advancing the Validation of Alternatives to Animal Testing. ALTEX 2024, 41: 545-566. doi:10.14573/altex.2410011.

Hartung T, Maertens A and Luechtefeld T. E-validation – Unleashing AI for Validation. ALTEX 2024, 41:567–587. doi: 10.14573/altex.2409211.

Hartung T, Schenke M and Smirnova L. Brain organoids as a translational model of human developmental neurotoxicology. In: Stem Cells in Neurotoxicology, Advances in Neurotoxicology, 2024 Chapter 3, Vol 12, 83-106. doi: 10.1016/bs.ant.2024.07.002.

Hartung T, Thornton JR and Smirnova L. Self-organizing human neuronal cultures in the modeling of environmental impacts on learning and intelligence. In: Advances in Neurotoxicology, 2024, Chapter 4, Volume 12, 107-135. Doi: 10.1016/bs.ant.2024.09.001.

Hartung T, Luechtefeld T and Maertens A. The role of AI in overcoming animal testing. In: Van Norman G and Krebs C. Moving Toward Nonanimal Approaches in Medical Research & Testing. Elsevier 2024, submitted. https://doi.org/10.14573/altex.2409211

Hartung T, Bajramovic JJ, Gibbs S and Corsini E. New Approach Methods in Immunology. Frontiers in Immunology 2024, 15:1488534. doi: 10.3389/fimmu.2024.1488534.

Kagan BJ, Mahlis M, Bhat A, Bongard J, Cole VM, Corlett P, Gyngell C, Hartung T, Jupp B, Levin M, Lysaght T, Opie N, Razi A, Smirnova L, Tennant I, Thestrup Wade P and, Ge Wang17. Towards a Nomenclature Consensus for Diverse Intelligent Systems: Call for Collaboration. The Innovation 2024, 5:1006589. https://doi.org/10.1016/j.xinn.2024.100658

Spiliotopoulos D, Koelbert C, Audebert M, Barisch I, Bellet D, Constans M, Czich A, Finot F, Gervais V, Khoury L, Kirchnawy C, Kitamoto S, Le Tesson A, Malesic L, Matsuyama R, Mayrhofer E, Mouche I, Preikschat B, Prielinger L, Rainer B, Roblin C, Wäse K. Assessment of the performance of the Ames MPF™ assay: A multicenter collaborative study with six coded chemicals. Mutat Res Genet Toxicol Environ Mutagen. 2024 Jan;893:503718. doi: 10.1016/j.mrgentox.2023.503718. Epub 2023 Nov 23. PMID: 38272629.

Mathisen GH, Bearth A, Jones LB, Hoffmann S, Vist GE, Ames HM, Husøy T, Svendsen C, Tsaioun K, Ashikaga T, Bloch D, Cavoski A, Chiu WA, Davies HG, Giusti A, Hartung T, Hirabayashi Y, Hogberg HT, Joglekar R, Kojima H, Krishnan K, Kwon S, Osborne OJ, Roggen E, Rooney AA, Rousselle C, Sass JB, Sepai O, Simanainen U, Thayer KA, Tong W, Wikoff D, Wright F and Whaley P. Time for CHANGE: system-level interventions for bringing forward the date of effective use of NAMs in regulatory toxicology. Archives of Toxicology 2024, 98:2299–2308, doi: 10.1007/s00204-024-03802-6.

Pamies D, Ekert J, Zurich M-G, Frey O, Werner S, Pergiovanni M, Freedman B, Teo AKK, Erfurth H, Reyes DR, Loskill P, Candarlioglu P, Suter-Dick L, Wang S, Hartung T, Coecke S, Stacey G, Wagegg BA, Dehne E-M, Pistollato F and Leist M. Recommendations on fit-for-purpose criteria to establish quality management for Microphysiological Systems (MPS) and for monitoring of their reproducibility. Stem Cell Reports, Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility, Stem Cell Reports 2024, 19:604-617. 10.1016/j.stemcr.2024.03.009.

Haber LT, Bradley MA, Buerger AN, Behrsing H, Burla S, Clapp PW, Dotson S, Fisher C, Genco KR, Kruszewski FH, McCullough SD, Page KE, Patel V, Pechacek N, Roper C, Sharma M and Jarabek AM (2024). New approach methodologies (NAMs) for the in vitro assessment of cleaning products for respiratory irritation: workshop report. Frontiers of Toxicology 6:1431790. doi: 10.3389/ftox.2024.1431790.

Sharma M, Huber E, Arnesdotter E, Behrsing HP, Bettman A, Constant S, Date R, Deshpande A, Fabian E, Gupta A, Gutierrez R, Gutleb A, Hargrove MM, Hollings M, Hutter V, Jarabek AM, Landsiedl R, Milchak L, Moyer RA, Murray JR, Page K, Patel M, Pearson SN, Petersen EJ, Reinke E, Roldan N, Roper C, Scaglione JB, Settivari RS, Verstaelen S, Wallace JL, McCullough S and Clippinger AJ (2024). Minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). Archives of Toxicology. https://doi.org/10.1007/s00204-024-03879-z.

Ward TA, Goldsby H, Connolly M, Roper C, Treasure C (2024). Predicting acute oral toxicity using AcutoX: an animal product-free and metabolically relevant human cell-based test. ALTEX. doi: 10.14573/altex.2311142.

Ejaz S, Roper C, Finlayson Z, Saitta KS, McCarthy T, Sun F, and Southall MD (2024). A comparative study of the in vitro dermal absorption of radiolabeled benzophenone through human skin. Toxicology In Vitro 98: 105835. doi: 10.1016/j.tiv.2024.105835.

Sewell F, Alexander-White C, Brescia S, Currie RA, Roberts R, Roper C, Vickers C, Westmoreland C, and Kimber I (2024). New approach methodologies (NAMs): identifying and overcoming hurdles to accelerated adoption. Toxicology Research, 13(2), tfae044. https://doi.org/10.1093/toxres/tfae044.

Sillé FCM, Busquet F, Fitzpatrick S, Herrmann K, Leenhouts-Martin L, Luechtefeld T, Maertens A, Miller GW, Smirnova L, Tsaioun K and Hartung T. The Implementation Moonshot Project for Alternative Chemical Testing (IMPACT) toward a Human Exposome Project. ALTEX 2024, 41:344–362. https://doi.org/10.14573/altex.2407081

Smirnova L and Hartung T. Creating Tiny Human “Organs” to Test medicines… and More!. Front. Young Minds. 2024, https://doi.org/10.3389/frym.2024.1320408

Smirnova L, Hogberg HT, Leist M and Hartung T. Revolutionizing Developmental Neurotoxicity Testing – a Journey from Animal Models to Advanced In Vitro Systems. ALTEX 2024, 41:152–178. doi: 10.14573/altex.2403281.

Sonnenburg A, van der Lugt B, Rehn J, Wittkowski P, Bech K, Padberg F, Eleftheriadou D, Dobrikov T, Bouwmeester H, Mereu C, Graf F, Kneuer C, Kramer NI, Blümmel T. Artificial intelligence-based data extraction for next generation risk assessment: Is fine-tuning of a large language model worth the effort? Toxicology. 2024 Nov;508:153933. doi: 10.1016/j.tox.2024.153933

Blümmel T, Rehn J, Mereu C, Graf F, Bazing F, Kneuer C, Sonnenburg A, Wittkowski P, Padberg F, Bech K, Eleftheriadou D, van der Lugt B, Kramer N, Bouwmeester H, Dobrikov T 2024. Exploring the use of Artificial Intelligence (AI) for extracting and integrating data obtained through New Approach Methodologies (NAMs) for chemical risk assessment. EFSA supporting publication 2024: 21(1):EN-8567. 400 pp. doi:10.2903/sp.efsa.2024.EN-8567

Blümmel T, Rehn J, Mereu C, Graf F, Kneuer C, Wittkowski P, Sonnenburg A, Moore A, Bech K, van der Lugt B, Bouwmeester H, Kramer N, Dobrikov T, 2022. Review of state-of-the-art AI tools and methods for screening and evaluating NAMs literature in the context of chemical risk assessment. EFSA supporting publication 2023: 20(1):EN-7815. 41 pp. doi:10.2903/sp.efsa.2023.EN-7815

Wollin KM, Batke M, Damm G, Freyberger A, Gundert-Remy U, Mangerich A, Hengstler JG, Partosch F, Schupp T, Sonnenburg A, Foth H. PFASs-restriction proposal commentary on ECHA’s Annex XV restriction report, proposal for a restriction, March 2023. Arch Toxicol. 2023 Dec;97(12):3305-3312. doi: 10.1007/s00204-023-03597-y

Sonnenburg A, Stahlmann R, Kreutz R, Peiser M. A new cell line based coculture system for skin sensitisation testing in one single assay using T cells, aryl hydrocarbon receptor knockout, and co-inhibitory blockage. Arch Toxicol. 2023 Jun;97(6):1677-1689. doi:10.1007/s00204-023-03506-3

Sonnenburg A, Stahlmann R, Kreutz R, Peiser M. Aryl hydrocarbon receptor knockout and antibody blockade of programmed cell death ligand1 increase co-stimulatory molecules on THP-1 and specific cytokine response of human T cells. Toxicol In Vitro. 2023 Feb;86:105502. doi: 10.1016/j.tiv.2022.105502

Svendsen C, Mathisen GH, Vist GE, Husøy T, Ames HM, Beronius A, Di Consiglio E, Druwe I, Hartung T, Hoffmann S, Hooijmans CR, Machera K, Robinson JF, Roggen E, Rooney AA, Roth N, Spilioti E, Spyropoulou A, Tcheremenskaia O, Testai E, Vinken M and Whaley P. Cross-mapping of terms used in chemical risk assessment with those used in systematic review: research protocol. Evidence-Based Toxicology 2024, 2(1). https://doi.org/10.1080/2833373x.2024.2371285

Vist GE, Ames HA, Mathisen GH, Husøy T, Svendsen C, Beronius A, Di Consiglio E, Druwe I, Hartung T, Hoffmann S, Hooijmans CR, Machera K, Prieto P, Robinson JF, Roggen E, Rooney AA, Roth N, Spilioti E, Spyropoulou A, Tcheremenskaia O, Testai E, Vinken M and Whaley P. A comprehensive item bank of internal validity issues of relevance to in vitro toxicology studies. Evidence-Based Toxicology, in press. https://doi.org/10.1080/2833373X.2024.2418045

Zeng X, Ma S, Luo Y, Zhang Y, Wang Q, Zhang Z, Ke W, Ma Y, Hu H, Hartung T, Wei Y and Zhong X. Environmentally Relevant Concentrations of Tetrabromobisphenol A Exposure Impends Neurovascular Formation through Perturbing Mitochondrial Metabolism in Zebrafish Embryos and Human Primary Endothelial Cells. Environ Sci Technol. 2024, 58:5267-5278. doi: 10.1021/acs.est.3c10132.

Juan-García, A.; Juan, C.; Taipale, S.; Vehniäinen ER. 2024. Beauvericin and enniatin B mycotoxins alter aquatic ecosystems: effects on green algae. Environmental Toxicology and Pharmacology, 107, 104415. https://doi.org/10.1016/j.etap.2024.104415

Mitchell, C.; Bridgeman, L.; Moyano-López, C.; Penalva-Olcina, R.; Juan, C.; Juan-García, A. 2024. Study of cytotoxicity in neuroblastoma cell line exposed to patulin and citrinin. Food Chem Toxicol 186, 114556. https://doi.org/10.1016/j.fct.2024.114556

Penalva-Olcina R., Juan C., Fernández-Franzón M., Juan-García A. 2024. Involvement of pro-inflammatory mediators in gliotoxin and ochratoxin A induced neurotoxicity and cell cycle disruption after individual and combined exposure. Toxic. Lett. 393, 24–32 https://doi.org/10.1016/j.toxlet.2024.01.009

Bridgeman, L.; Juan, C.; Berrada, H.; Juan-García, A. 2024. Effect of acrylamide and mycotoxins in SH-SY5Y cells: A Review. Toxins 16, 87. https://doi.org/10.3390/toxins16020087

Kandarova H. . (2024). Organ-on-a-chip as novel tox testing tools. In Encyclopedia of Toxicology: Fourth edition. Vol. 1-9. – Elsevier-Academic Press, 2024, vol. 7., p. 173-176. ISBN 978-0-323-85434-4. https://doi.org/10.1016/B978-0-12-824315-2.01037-X

Kandarova H. (2024). New approach methods (NAMs) for multiple non-animal based test methods. In Encyclopedia of Toxicology: Fourth edition. Vol. 1-9. – Elsevier-Academic Press, 2024, vol. 6, p. 741-746. ISBN 978-0-323-85434-4. https://doi.org/10.1016/B978-0-12-824315-2.01036-8

Kandarova H. (2024), Roper C, Dvorakova M. (2024). ESTIV 2022: Key enabling non-animal technologies for research, education and testing : editorial. In Toxicology in vitro : the official journal of the European Society for Toxicology in Vitro, 2024, vol. 96, art. no. 105779. ISSN 0887-2333. https://doi.org/10.1016/j.tiv.2024.105779

Kandarova H, Pobis P. (2024). The “Big Three” in biocompatibility testing of medical devices: implementation of alternatives to animal experimentation- are we there yet? In Frontiers in Toxicology, 2023, vol. 5, art. no. 1337468. ISSN 2673-3080. https://doi.org/10.3389/ftox.2023.1337468

Kleinstreuer N and Hartung T. Artificial Intelligence (AI) – it’s the end of the tox as we know it (and I feel fine) – AI for Predictive Toxicology. Archives in Toxicology 2024, 98:735–754. Doi:10.1007/s00204-023-03666-2.

Laure-Alix Clerbaux, Julija Filipovska, Penny Nymark, Vinita Chauhan, Katherina Sewald, Miriam Alb, Madgalini Sachana, Anna Beronius, Maria-Joao Amorim, Clemens Wittwehr. Beyond chemicals: Opportunities and challenges of integrating non-chemical stressors in adverse outcome pathways. ALTEX 2023 Nov 17. https://doi.org/10.14573/altex.2307061

Maertens A, Antignac E, Benfenati E, Bloch D, Fritsche E, Hoffmann S, Jaworska J, Loizou G, McNally K, Piechota P, Roggen EL, Teunis M and Hartung T. The probable future of toxicology – probabilistic risk assessment. ALTEX 2024, in press. doi: 10.14573/altex.2310301.

Mathisen GH, Vist GE, Whaley P, White RA, Husøy T, Ames HM, Beronius A, Di Consiglio E, Druwe I, Hartung T, Hoffmann S, Hooijmans CR, Machera K, Prieto P, Robinson JF, Roggen E, Rooney AA, Roth N, Spilioti E, Spyropoulou A, Tcheremenskaia O, Testai E, Vinken M and Svendsen C. Protocol: Testing the performance of INVITES-IN, a tool for 1 assessing the internal validity of in vitro studies 2. Evidence-Based Toxicology, in press. DOI:10.1080/2833373X.2023.2293289

Page K, Westerink W, Sullivan K, McDonald T, Roper C. Assessment of the utility of the novel Phenion® full thickness human skin model for detecting the skin irritation potential of antimicrobial cleaning products. Toxicol In Vitro. 2024, Vol 94. https://doi.org/10.1016/j.tiv.2023.105726

Martínez-Alonso, C., Izzo, L., Rodríguez-Carrasco, Y., Ruiz, MJ. Integrated Approach to Cyclopiazonic Acid Cytotoxicity Using In Vitro (2D and 3DModels) and In Silico Methods. Toxins 2024, 16, 473. https://doi.org/ 10.3390/toxins16110473

Franco-Campos,F., Fernández-Franzón,M., Rodríguez-Carrasco,Y., Ruiz, MJ. 2024. Amitraz mechanisms of cytotoxicity in a characterized SH-SY5Y cells spheroid. Model. Toxicology 509, 153987, https://doi.org/10.1016/j.tox.2024.153987

Taroncher, M., Zingales, V., Rodríguez-Carrasco, Y., Ruiz, MJ. 2024. Identification of Biotransformation Products of T-2 Toxin in HepG2 Cells Using LC-Q-TOF MS. Foods, 13 (10), 1501. https://doi.org/10.3390/foods13101501

Martí-Quijal, FJ., Castagnini, JM., Barba, FJ., Ruiz, MJ. 2024. Effect of Spirulina and Fish Processing By-Products Extracts on Citrinin-Induced Cytotoxicity in SH-SY5Y Cells. Foods, 13 (12), 1932. https://doi.org/10.3390/foods13121932

Zingales, V., Esposito, MR., Quagliata, M., Cimetta, E., Ruiz, M.J. 2024. Comparative Study of Spheroids (3D) and Monolayer Cultures (2D) for the In Vitro Assessment of Cytotoxicity Induced by the Mycotoxins Sterigmatocystin, Ochratoxin A and Patulin. Foods, 13 (4), 564. https://doi.org/10.3390/foods13040564

Montanarí, C., Franco-Campos, F., Taroncher, M., Rodríguez-Carrasco, Y., Zingales, V., Ruiz, MJ. 2024. Chlorpyrifos induces cytotoxicity via oxidative stress and mitochondrial dysfunction in HepG2 cells. Food Chem. Toxicol., 192, 114933. https://doi.org/10.1016/j.fct.2024.114933

Taroncher, M., Gonzalez-Suarez, AM., Gwon, K., Romero, S., Reyes-Figueroa, AD., Rodríguez-Carrasco, Y., Ruiz, MJ., Stybayeva, G., Revzin, A., de Hoyos-Vega, JM. 2024. Using Microfluidic Hepatic Spheroid Cultures to Assess Liver Toxicity of T-2 Mycotoxin. Cells, 13 (11), 900. https://doi.org/10.3390/cells13110900

Zingales, V., Esposito, MR., Quagliata, M., Cimetta, E., Ruiz, MJ. 2024. Cytotoxic effects induced by combined exposure to the mycotoxins sterigmatocystin, ochratoxin A and patulin on human tumour and healthy 3D spheroids. Food Chem. Toxicol., 192, 114951. https://doi.org/10.1016/j.fct.2024.114951

Taroncher, M., Fuentes, C., Rodríguez-Carrasco, Y., Ruiz, MJ. 2024. Assessment of the genotoxic and mutagenic effects induced by T-2 mycotoxin in HepG2 cells. Toxicology, 501, 153712. https://doi.org/10.1016/j.tox.2023.153712

Martínez-Alonso, C., Taroncher, M., Rodríguez-Carrasco, Y., Ruiz, MJ. 2023. Evaluation of the Bioaccessible Fraction of T-2 Toxin from Cereals and Its Effect on the Viability of Caco-2 Cells Exposed to Tyrosol. Toxins, 15 (8), 493. https://doi.org/10.3390/toxins15080493

Zingales, V., Esposito, M. R., Torriero, N., Taroncher, M., Cimetta, E., Ruiz, MJ. 2023. The Growing Importance of Three-Dimensional Models and Microphysiological Systems in the Assessment of Mycotoxin Toxicity. Toxins, 15 (7), 422. https://doi.org/10.3390/toxins15070422

Taroncher, M., Rodríguez-Carrasco, Y., Barba, F. J., Ruiz, MJ. 2023. Enhancement of the Antioxidant Effect of Natural Products on the Proliferation of Caco-2 Cells Produced by Fish Protein Hydrolysates and Collagen. International J. Mol. Sci., 24 (7), 6871. https://doi.org/10.3390/ijms24076871

Giorgini, M., Taroncher, M., Tolosa, J., Ruiz, MJ., Rodríguez-Carrasco, Y. 2023. Amitraz and its metabolites: oxidative stress-mediated cytotoxicity in HepG2 cells and study of their stability and characterization in honey. Antioxidants, 12, 885. https://doi.org/10.3390/antiox12040885

Taroncher, M.,Rodríguez-Carrasco, Y., Barba, FJ., Ruiz MJ. 2023. Evaluation for cytotoxicity, analysis of metals and cumulative risk assessment in microalgae. Toxicol. Mech. Meth., 33, 388-400. https://doi.org/10.1080/15376516.2022.2152514

Giorgini, M., Taroncher, M., Ruiz, MJ., Rodríguez-Carrasco, Y., Tolosa, J. 2023. In Vitro and Predictive Computational Toxicology Methods for the Neurotoxic Pesticide Amitraz and Its Metabolites. Brain Sci., 13, 252. https://doi.org/10.3390/brainsci13020252

Sillé F and Hartung T. Metabolomics in Preclinical Drug Safety Assessment: Current Status and Future Trends. Metabolites 2014,14:98. https://doi.org/10.3390/metabo14020098.

Smirnova L and Hartung T. The Promise and Potential of Brain Organoids. Advanced Healthcare Materials, 2024, 2302745. https://doi.org/10.1002/adhm.202302745.

Smirnova L and Hartung T. Microphysiological systems (MPS) – engineering tiny pieces of human organs to test drugs. Frontiers for Young Minds 2024, in press. https://doi.org/10.1111/acel.14070

2023

Abass K, Reponen P, Anyanwu B, Pelkonen O. Inter-species differences between humans and other mammals in the in vitro metabolism of carbofuran and the role of human CYP enzymes. Environ Toxicol Pharmacol. 2023 Sep;102:104243. doi: 10.1016/j.etap.2023.104243.

Altmaier S, Meiser I, Lemesre E, Chanrion B, Steeg R, Leonte LE, Holst B, Nielsen BS, Clausen C, Schmidt K, Bur S, Vinggaard AM, Zimmermann H, Neubauer JC, Rasmussen MA. Human iPSC-derived hepatocytes in 2D and 3D suspension culture for cryopreservation and in vitro toxicology studies. Reprod. Tox., 111, 68-80, 2022. https://doi.org/10.1016/j.reprotox.2022.05.00

Ashammakhi N, Nakipoglu M, Hartung T. Lessons, insights from the first Microphysiological World Summit. Journal of Cranofacial Surgery Open 2023, 1:e0011.
DOI: 10.1097/SC9.0000000000000011.

Barreras P, Pamies D, Hartung T, Pardo CA. Human brain microphysiological systems in the study of neuroinfectious disorders. Experimental Neurology 365:114409. Doi: 10.1016/j.expneurol.2023.114409

Bishop PL, Brescia S, Brunner R, Casey W, Conlee-Griffin K, Craenen K, Currie RA, Domoradzki J, Embry M, Harris MI, Hartung T, Hilton GM, Hooberman B, Ingle B, Jang K-J, Kinter L, Krall C, Leedale J, Lowit A, Mehta J, Mendez E, Mingoia B, Munarriz E, Murphy L, Myer A, Ottoni A, Panzarea M, Perron M, Pina J, Ramsingh D, Sewell F, Swanson J, Tan C, Tan Y-M, Terron A, Trainer MA, Valadares MC, Webb S, Webb E, Willett C, Wolf DC. Challenges and opportunities for overcoming dog use in agrochemical evaluation and registration. ALTEX 2023, 40:534-540. doi: 10.14573/altex.2302151.

Butera A, Smirnova L, Ferrando-May E, Hartung T, Brunner T, Leist M, Amelio I. Deconvoluting Gene and Environment interactions to develop “epigenetic score meter” of disease. EMBO Molecular Medicine 2023, e18208. https://doi.org/10.15252/emmm.202318208.

Caloni F, De Angelis I, Gribaldo L, Heinonen T, Kandarova H, Kral V, Letasiova S, Sillé F, Smirnova L, Pilar Vinardell M, Hartung T. Women in Alternatives. ALTEX 2023, 40:545-548, doi: 10.14573/altex.2303211

CALONI, Francesca – DE ANGELIS, Isabella – GRIBALDO, Laura – HEINONEN, Tuula – KANDAROVA, Helena – KRAL, Vivian – LETAŠIOVÁ, Silvia – SILLÉ, Fenna – SMIRNOVA, Lena – VINARDELL, Maria Pilar – HARTUNG, Thomas. Women in Alternatives. In ALTEX : Alternatives to Animal Experimentation, 2023, vol. 40, no. 3, pp. 545-548. . ISSN 1868-596X. https://doi.org/10.14573/altex.2303211

De Silva A, Ramesh R, Ungar L, Shuler HM, Cowan NJ, Platt M, Li C, Isik L, Roh S-E, Charles A, Venkataraman A, Caffo B, How JJ, Kebschull JM, Krakauer JW, Bichuch M, Kinfu KA, Yezerets E, Jayaraman D, Shin JM, Villar S, Phillips I, Priebe CE, Hartung T, Miller MI, Vidal R, Dey J, Huang N, Eaton E, Etienne-Cummings R, Ogburn EL, Burns R, Osuagwu O, Mensh B, Muotri AR, Brown J, White C, Yang W, Rusu AA, Verstynen T, Kording KP, Chaudhari P, Vogelstein JT. Prospective Learning: Principled Extrapolation to the Future. Conference on Lifelong Learning Agents, 2023. https://doi.org/10.48550/arXiv.2201.07372

Diemar M, Vinken M, Teunis M, Krul C, Busquet F, Zajac J, Kandarova H, Corvi R, Russo M, Kharina A, Bryndum L, Santillo M, Bloch D, Kucheryavenko O, Panagiotakos D, Rogiers V, Beekhuijzen M, Giusti A, Najjar A, Courage C, Koenig T, Kolle S, Boonen H, Dhalluin S, Boberg J, Müller B, Kukic P, Ritskes-Hoitinga M, Grasselli E, Zietek T, Stoddart G, Heusinkveld H, Castell J, Benfenati E, Yang H, Perera S, Paini A, Kramer N, Hartung T, Janssen M, Fritsche E, Jennen D, Piumatti M, Rathman J, Marusczyk J, Milec L,, Roggen E. Report of the first ONTOX Stakeholder Network meeting: digging under the surface of ONTOX together with the stakeholders. ATLA, in press. https://doi.org/10.1177/02611929231225730

Horánszky A, Shashikadze B, Elkhateib R, Lombardo SD, Lamberto F, Zana M, Menche J, Fröhlich T, Dinnyés A. Proteomics and disease network associations evaluation of environmentally relevant Bisphenol A concentrations in a human 3D neural stem cell model. Front Cell Dev Biol. 2023;11. https://doi.org/10.3389/fcell.2023.1236243

Kistamás K, Müller A, Muenthaisong S, Lamberto F, Zana M, Dulac M, Leal F, Maziz A, Costa P, Bernotiene E, Bergaud C, Dinnyés A. Multifactorial approaches to enhance maturation of human iPSC-derived cardiomyocytes. J Mol Liq. 2023;387. https://doi.org/10.1016/j.molliq.2023.122668

Lamberto F, Shashikadze B, Elkhateib R, Lombardo SD, Horánszky A, Balogh A, Kistamás K, Zana M, Menche J, Fröhlich T, Dinnyés A. Low-dose Bisphenol A exposure alters the functionality and cellular environment in a human cardiomyocyte model. Environmental Pollution. 2023;335. https://doi.org/10.1016/j.envpol.2023.122359

Jennings P, Carta G, Singh P, da Costa Pereira D, Feher A, Dinnyes A, Exner TE, Wilmes A. Capturing time-dependent activation of genes and stress-response pathways using transcriptomics in iPSC-derived renal proximal tubule cells. Cell Biol Toxicol. 2023;39:1773–93. https://doi.org/10.1007/s10565-022-09783-5

dos Santos Rodrigues B., Leroy K., Mihajlovic M., De Boever S., Vanbellingen S., Cogliati B., Aerts J.L., Vinken M. (2023) “Evaluation of functional candidate biomarkers of non-genotoxic hepatocarcinogenicity in human liver spheroid co-cultures.” Archives of Toxicology 97: 1739-1751 (impact factor₂₀₂₂: 6.1). PMID: 36941454, DOI: 10.1007/s00204-023-03486-4

Dvořáková M, Svobodová L, Rucki M, Ševčík V, Hošíková B, Chrz J, Bendová H, Kejlová K, Očadlíková D, Malý M, Kolářová H, Mannerström M, Kanďárová H, Jírová D. The Safety Assessment of Cosmetic Perfumes by Using In Chemico and In Vitro Methods in Combination with GC-MS/MS Analysis. Altern Lab Anim. 2023 Jul;51(4):224-248. https://journals.sagepub.com/doi/10.1177/02611929231184635

EFSA Panel on Plant Protection Products and their Residues (PPR); Hernandez-Jerez A, Adriaanse P, Aldrich A, Berny P, Coja T, Duquesne S, Focks A, Marinovich M, Millet M, Pelkonen O, Pieper S, Topping C, Widenfalk A, Wilks M, Wolterink G, Kasteel R, Kuppe K, Tiktak A. Statement of the Scientific Panel on Plant Protection Products and their Residues (PPR Panel) on the design and conduct of groundwater monitoring studies supporting groundwater exposure assessments of pesticides. EFSA J. 2023 May 15;21(5):e07990. doi: 10.2903/j.efsa.2023.7990. eCollection 2023 May.

EFSA Panel on Plant Protection Products and their Residues (PPR); Hernandez-Jerez AF, Adriaanse P, Aldrich A, Berny P, Coja T, Duquesne S, Focks A, Millet M, Pelkonen O, Pieper S, Tiktak A, Topping CJ, Widenfalk A, Wilks M, Wolterink G, Angeli K, Recordati C, Van Durseen M, Aiassa E, Lanzoni A, Lostia A, Martino L, Guajardo IPM, Panzarea M, Terron A, Marinovich M. Development of adverse outcome pathways relevant for the identification of substances having endocrine disruption properties Uterine adenocarcinoma as adverse outcome. EFSA J. 2023 Feb 14;21(2):e07744. doi: 10.2903/j.efsa.2023.7744.

Escher BI, Lamoree M, Antignac J-P, Scholze M, Herzler M, Hamers T, Jensen TK, Audebert M, Busquet F, Maier D, Oelgeschläger M, Valente MJ, Boye H, Schmeisser S, Dervilly G, Piumatti M, Moteau S, König M, Renko K, Jornet MM, Cariou R, Ma Y, Treschow AF, Kortenkamp A, Vinggaard AM. Mixture Risk Assessment of Complex Real-Life Mixtures—The PANORAMIX Project. Int. J. Environ. Res. Public Health 19(20), 12990, 2022. https://doi.org/10.3390/ijerph192012990

Ezechiáš M, The agonistic bioanalytical equivalent concentration: A novel tool for assessing the endocrine activity of environmental mixtures. Environ Toxicol Pharm. 2022 Jan; 89:103781. https://doi.org/10.1016/j.etap.2021.103781

Cantalapiedra F., Juan-García A. and Juan, C. 2023. Perception of food safety of entomophagy among higher education students: exploring insects as a novel food source. Foods 2023, 12, 4427. https://doi.org/10.3390/foods12244427

Bridgeman L., Juan C., Juan-García A., Berrada H. 2023. The individual and combined effect of Acrylamide, Fumitremorgin C and Penitrem A on human neuroblastoma SH-SY5Y cells. Food Chem Toxicol 182, 114114. https://doi.org/10.1016/j.fct.2023.114114

Sebastià A., Pallares N., Bridgeman L., Juan-García A., Castagnini J.M., Ferrer E., Barba F.J., Berrada H. 2023. A critical review of acrylamide green extraction and determination in food matrices: Current insights and future perspectives. TrAc Trends in Analytical Chemistry 167, 117267. https://doi.org/10.1016/j.trac.2023.117267

Penalva-Olcina R., Juan C., Fernández-Franzón M., Juan-García A. 2023. Cell cycle and enzymatic activity alterations induced by ROS production in human neuroblastoma cells SH-SY5Y exposed to Fumonisin B1, Ochratoxin A and their combination. Toxicol. In vitro 93, 105670. https://doi.org/10.1016/j.tiv.2023.105670

Juan-García A., Pakkanen H., Juan C., Vehniäinen ER. 2023. Alterations in Daphnia magna exposed to enniatin B and beauvericin provide additional value as environmental indicators. Ecotoxicology and Environmental Safety, 249, 114427. https://doi.org/10.1016/j.ecoenv.2022.114427

Álvarez-Ortega N., Caballero-Gallardo K., Juan C., Juan-García A., Olivero-Verbel J., 2023. Cytoprotective, antiproliferative, and anti-oxidant potential of the hydroethanolic extract of Fridericia chica leaves on human cancer cell lines exposed to α- and β-zearalenone. Toxins, 15, 36. https://doi.org/10.3390/toxins15010036

Golden E, Ukaegbu DC, Ranslow P, Brown R, Hartung T, Maertens A. The Good, The Bad, and The Perplexing: Structural Alerts and Read-Across for Predicting Skin Sensitization Using Human Data. Chem. Res. Toxicol. 2023, 36:734–746. Doi: 10.1021/acs.chemrestox.2c00383.

Alam El Din D-M, Monkemoller L, Loeffler A, Habibollahi F, Schenkman J, Mitra A, van der Molen T, Ding L, Laird J, Schenke M, Johnson EC, Kagan BJ, Hartung T and Smirnova L. Human Neural Organoid Microphysiological System Shows the Building Blocks Necessary for Basic Learning and Memory. https://www.biorxiv.org/content/10.1101/2024.09.17.613333v1.full

Gori GB, Aschner M, Borgert CJ, Cohen SM, Dietrich DR, Galli CL, Greim H, Heslop-Harrison JS, Kacew S, Kaminski NE, Klaunig JE, Marquardt HWJ, Pelkonen O, Roberts R, Savolainen KM, Tsatsakis A, Yamazaki H. US regulations to curb alleged cancer causes are ineffectual and compromised by scientific, constitutional and ethical violations. Arch Toxicol. 2023 Jun;97(6):1813-1822. doi: 10.1007/s00204-022-03429-5.

Hartung T. A call for a Human Exposome Project. ALTEX 2023, 40:4–33. doi: 10.14573/altex.2301061.

Hartung T. ToxAIcology – AI as the New Frontier in Chemical Risk Assessment. Frontiers in AI, Sec. Medicine and Public Health 2023, 40:559–570. doi: 10.3389/frai.2023.1269932

Hartung T, Smirnova L, Morales Pantoja IE Akwaboah A, Alam El Din D-M, Berlinicke CA, Boyd JL, Caffo BS, Cappiello B, Cohen-Karni T, Curley L, Etienne-Cummings R, Dastgheyb R, Gracias DH, Gilbert F, Habela CW, Han F, Harris T, Herrmann K, Hill E, Huang Q, Jabbour RE, Johnson EC, Kagan BJ, Krall C, Levchenko A, Locke P, Maertens A, Metea M, Muotri AR, Parri R, Paulhamus BL, Plotkin J, Roach P, Romero JC, Schwamborn JC, Sillé F, Szalay A, Tsaioun K, Tornero D, Vogelstein JT, Wahlin K,, Zack DJ. The Baltimore Declaration toward the exploration of organoid intelligence. Frontiers in Science 2023, 1:1017235. doi: 10.3389/fsci.2023.1017235

Hartung T. Can you take AI out of the wild and should you? Frontiers Policy Labs 2023, https://policylabs.frontiersin.org/content/commentary-ai-data-thomas-hartung

Hartung T. ToxAIcology – the Evolving Role of Artificial Intelligence in Advancing Toxicology and Modernizing Regulatory Science. ALTEX 2023, 40: 559-570. doi: https://10.14573/altex.2309191.

Hartung T. Book Review: Rat Trap – Breaking Free from the Illusion of Progress in Animal Research by Pandora Pound”, ALTEX 2023, 40:705. Available at: https://www.altex.org/index.php/altex/article/view/2697

Holzer A-K, Dreser N, Pallocca G, Mangerich A, Stacey G, Dipalo M, van de Water B, Rovida C, Wirtz PH, van Vugt B, Panzarella G, Hartung T, Terron A, Mangas I, Herzler M, Marx-Stoelting P, Coecke S, Leist M. Acceptance criteria for new approach methods in toxicology and human health-relevant life science research – part I, ALTEX 2023, 40:706–712. doi: 10.14573/altex.2310021.

Jiang J., van Ertvelde J., Ertaylan G., Peeters R., Jennen D., de Kok T.M., Vinken M. (2023) “Unraveling the mechanisms underlying drug-induced cholestatic liver injury: identifying key genes using machine learning techniques on human in vitro data sets.” Archives of Toxicology 97: 2969-2981 (impact factor₂₀₂₂: 6.1). PMID: 37603094, PMCID: PMC10504391, DOI: 10.1007/s00204-023-03583-4

Jordan R, Ford-Scheimer SL, Alarcon RM, Atala A, Borenstein JT, Brimacombe KR, Cherry S, Clevers H, Davis MI, Funnell SGP, Gehrke L, Griffith LG, Grossman AC, Hartung T, Ingber DE, Kleinstreuer NC, Kuo CJ, Lee EM, Mummery CL, Pickett TE, Ramani S, Rosado-Olivieri EA, Struble EB, Wan Z, Williams MS, Hall MD, Ferrer M, Markossian S. Report of the Assay Guidance Workshop on 3D Tissue Models for Antiviral Drug Development. J. Inf. Dis. 2023, 228:S337–S354, doi: 10.1093/infdis/jiad334

Kincaid B, Piechota P, Golden E, Maertens M, Hartung T, Maertens A. When exposures are more than the sum of their parts: using in silico tools to predict flame retardant metabolites for more informative exposomics-based approaches. Front. Toxicol., Sec. Environmental Toxicology 2023, 5. doi: 10.3389/ftox.2023.1216802

Knight J, Hartung T, Rovida C. 4.2 million and counting…the animal toll for REACH systemic toxicity studies. ALTEX 2023, 40:389-407. Doi: 10.14573/altex.2303201.

Lauschke K, Dalgaard Md, Emnéus J, Vinggaard AM. Transcriptomic changes upon epoxiconazole exposure in a human stem cell-based model of developmental toxicity. Chemosphere, 284, 131225, 2021. https://doi.org/10.1016/j.chemosphere.2021.13122

Lauschke K, Volpini L, Liu Y, Vinggaard AM, Hall VJ. A comparative assessment of marker expression between cardiomyocyte differentiation of human iPSCs and the developing pig heart. Stem Cells and Development 30 (7), 374-385, 2021. doi:10.1089/scd.2020.0184

Lauschke K, Treschow AF, Rasmussen MA, Davidsen N, Holst B, Emnéus JK, Taxvig C and Vinggaard AM. Creating a human NKX2.5 reporter stem cell line for developmental toxicity testing. Arch Toxicol 95, 1659–1670, 2021. doi:10.1007/s00204-021-03018-y

Ma Y, Taxvig C, Rodríguez-Carrillo A, Mustieles V, Reiber L, Kiesow A, Löbl NM, Fernández MF, Alstrup Hansen TV, Valente MJ and Vinggaard AM. Human risk associated with exposure to mixtures of antiandrogenic chemicals evaluated using in vitro hazard and human biomonitoring data. Environ. Int. 173, 107815, 2023. https://doi.org/10.1016/j.envint.2023.107815

Menghini M., Pedrazzani R, Feretti D, Mazzoleni G, Steimberg N, Urani, C, Zerbini I. and Bertanza G. Beyond the Black Box of Life Cycle Assessment in Wastewater Treatment Plants: Which Help from Bioassays? WATER 2023 15(5) https://doi.org/10.3390/w15050960.

Morales Pantoja IE, Smirnova L, Muotri AR, Wahlin KJ, Kahn J, Boyd L, Gracias DH, Cohen-Karni T, Caffo BS, Szalay AS, Han F, Zack DJ, Etienne-Cummings R, Akwaboah A, Romero JC, Alam El Din D-M, Plotkin JD, Paulhamus BL, Johnson EC, Gilbert F, Curley JL, Cappiello B, Schwamborn JC, Hill EJ, Roach P, Tornero D, Krall C, Parri R, Sillé F, Levchenko A, Jabbour RA, Kagan BJ, Berlinicke CA, Huang Q, Maertens A, Herrmann K, Tsaioun K, Dastgheyb R, Habela CW, Vogelstein JT, Hartung T. First Organoid Intelligence (OI) Workshop to Form an OI Community, Frontiers Artificial Intelligence 2023, Section Organoid Intelligence, 6: 1116870. doi: 10.3389/frai.2023.1116870.

Najjar A, Kramer N, Gardner I, Hartung T, Steger-Hartmann T. Perspectives in Predictive Toxicology. Front. Pharmacol. 2023, Sec. Predictive Toxicology 14: 1257423. doi: 10.3389/fphar.2023.1257423

Naik NN, Vadloori B, Poosala S, Srivastava P, Coecke S, Smith A, Akhtar A, Roper C, Radhakrishnan S, Bhyravbhatla B, Damle M, PullaVK, Hackethal J, Horland R, Li AP, Pati F, Singh MS, Occhetta P, Bisht R, Dandekar P, Bhagavatula K, Pajkrt D, Johnson M, Weber T, Huang J, Hysenaj L, Mallar B, Ramray B, Dixit S, Joshi S, and Kulkarni K (2023). Advances in animal models and cutting-edge research in alternatives: proceedings of the third international conference on 3Rs research and progress, Vishakhapatnam, 2022. Alternatives to Laboratory Animals 51(4): 263-288. https://doi:10.1177/02611929231180428

Panzarea M, Terron A, Coja T, Pelkonen O. Editorial: Relevance of dog studies for the derivation of health-based guidance values for plant protection products approval. EFSA J. 2022 Sep 22;20(9):e200923. doi: 10.2903/j.efsa.2022.e200923.

Pelkonen O, Abass K, Parra Morte JM, Panzarea M, Testai E, Rudaz S, Louisse J, Gundert-Remy U, Wolterink G, Jean-Lou Cm D, Coecke S, Bernasconi C. Metabolites in the regulatory risk assessment of pesticides in the EU. Front Toxicol. 2023 Dec 19;5:1304885. doi: 10.3389/ftox.2023.1304885. Review.

Roper CS, and Neill DR. (2023). Transitioning Acute In Vitro Inhalation Toxicology Testing to Chronic and Repeat Dose Testing; the Challenge of Mucus Depletion in Upper Airway Test Systems and Use of Sputum Mimics. Open Acc J of Toxicol. 5(4):555669. DOI:10.19080/JOJDC.2023.05.555669.

Rodriguez-Carillo et al. Implementation of effect biomarkers in human biomonitoring studies: A systematic approach synergizing toxicological and epidemiological knowledge. Int J Hy Environ Health, 249, 114140, 2023. doi: 10.1016/j.ijheh.2023.114140.

Rovida C, Busquet F, Leist M, Hartung T. REACH out-numbered! The future of REACH and animal numbers. ALTEX 2023, 40:367-388. Doi: 10.14573/altex.2307121

Schmeisser S, Miccoli A, von Bergen M, Berggren E, Braeuning A, Busch W, Desaintes C, Gourmelon A, Grafström R, Harrill J, Hartung T, Herzler M, Kass G, Kleinstreuer N, Leist M, Luijten M, Marx-Stoelting P, Poetz O, van Ravenzwaay B, Roggeband R, Rogiers V, Roth A, Sanders P, Thomas RS, Vinggaard AM, Vinken M, van de Water B, Luch A, Tralau T. New Approach Methodologies in human regulatory toxicology – not if, but how and when!, Environment International 2023, 178:108082. doi: 10.1016/j.envint.2023.108082

Smirnova L, Caffo BS, Gracias DH, Huang Q, Morales Pantoja IE, Tang B, Zack DJ, Berlinicke CA, Boyd JL, Harris TD, Johnson EC, Kagan BJ, Kahn J, Muotri AR, Paulhamus BL, Schwamborn JC, Plotkin J, Szalay AS, Vogelstein JT, Worley PF, Hartung T. Organoid intelligence (OI): the new frontier in biocomputing and intelligence-in-a-dish, Frontiers in Science, 2023, 1:1017235. doi: 10.3389/fsci.2023.1017235.

Smirnova L, Morales Pantoja IE, Hartung T. Brain-cell cultures: the future of computers and more? Frontier for Young Minds 2023, 11:1049593. doi: 10.3389/frym.2023.1049593.

Smirnova L, Morales Pantoja IE, Hartung T. Organoid Intelligence (OI) – the ultimate functionality of a brain microphysiological system. ALTEX 2023, 40:191-203. doi:10.14573/altex.2303261

L. Svobodova, K. Kejlova, M. Rucki, J. Chrz, P. Kubincova, M. Dvorakova, H. Kolarova, D. Jirova. Health safety of parabens evaluated by selected in vitro methods. Regulatory Toxicology and Pharmacology, Volume 137, 2023, 105307, ISSN 0273-2300. https://doi.org/10.1016/j.yrtph.2022.105307.

van Ertvelde J., Verhoeven A., Maerten A., Cooreman A., dos Santos Rodrigues B., Sanz Serrano J., Mihajlovic M., Tripodi I., Teunis M., Jover R., Luechtefeld T., Vanhaecke T., Jiang J., Vinken M. (2023) “Optimization of an adverse outcome pathway network on chemical-induced cholestasis using an artificial intelligence-assisted data collection and confidence level quantification.” Journal of Biomedical Informatics 145: 104465 (impact factor₂₀₂₂: 4.5). PMID: 37541407, DOI: 10.1016/j.jbi.2023.104465

VISKUPIČOVÁ, Jana – REZBÁRIKOVÁ, Petronela – KOVÁČIKOVÁ, Lucia – KANDAROVA, Helena – MÁJEKOVÁ, Magdaléna. Inhibitors of SARS-CoV-2 main protease: Biological efficacy and toxicity aspects. In Toxicology in vitro: the official journal of the European Society for Toxicology in Vitro, 2023, vol. 92, art. no. 105640. ISSN 0887-2333. Dostupné na: https://doi.org/10.1016/j.tiv.2023.105640

Turner J, Pound P, Owen C, Hutchinson I, Hop M, Chau DYS, Barrios Silva LV, Coleman M, Dubourg A, Harries LW, Hutter V, Kenna JG, Lauschke VM, Neuhaus W, Roper C, Watkins PB, Welch J, Alvarez LR and Taylor K (2023). Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment. ALTEX 40(3): 519-533. doi: 10.14573/altex.2212081.

Wallace J, Jackson GR, Kaluzhny Y, Ayehunie S, Lansley AB, Roper C, and Hayden PJ. (2023). Evaluation of in vitro rat and human airway epithelial models for acute inhalation toxicity testing. Toxicological Sciences 194 (2); 178‑190. doi: 10.1093/toxsci/kfad058.

2022

Abass K, Reponen P, Alsanie WF, Rautio A, Pelkonen O. Characterization of furathiocarb metabolism in in-vitro human liver microsomes and recombinant cytochrome P450 enzymes. Toxicol Rep. 2022 Apr 1;9:679-689. doi: 10.1016/j.toxrep.2022.03.046.

Abass K, Reponen P, Alsanie WF, Rautio A, Pelkonen O. Metabolic profiling and in vitro-in vivo extrapolation of furathiocarb in mammalian hepatic microsomes. Toxicol Rep. 2022 Mar 29;9:750-758. doi: 10.1016/j.toxrep.2022.03.030.

Barreras P, Pamies D, Monaco MC, Munoz LS, Zhong X, Major EO, Hogberg HT; Hartung T, Pardo CA. A human derived 3D brain microphysiological system as a model to study JC virus infection. Journal Neurovirology 2022, 28:17-26..doi: 10.1007/s13365-022-01062-7.

Bertanza G, Steimberg N, Pedrazzani R, Boniotti J, Ceretti E, Mazzoleni, G, Menghini M, Urani C, Zerbini I, Feretti D. Wastewater toxicity removal: Integrated chemical and effect-based monitoring of full-scale conventional activated sludge and membrane bioreactor plants. Sci Total Environ. 2022 851(Part 1, 10 December 2022), 1-12 https://doi.org/10.1016/j.scitotenv.2022.158071

Caloni F, De Angelis I, Hartung T. Replacement of animal testing by integrated approaches to testing and assessment (IATA): a call for in vivitrosi. Archives of Toxicology 2022, 96:1935-1950. doi: 10.1007/s00204-022-03299-x.

Fossati P, Hartung T, Martino PA, Mormino G, Vitale A, De Angelis I. Summer Meeting Alternative Methods, CitiZen Science. ALTEX 2023, 39:159–160. https://doi.org/10.14573/altex.2112201

Deng J, Hartung T, Capobianco E, Chen JY, Emmert-Streib F. Artificial Intelligence for Precision Medicine. Front. Artif. Intell. 4:834645. doi: 10.3389/frai.2021.834645

EFSA Panel on Plant Protection Products and their Residues (PPR); Hernandez Jerez A, Adriaanse P, Berny P, Coja T, Duquesne S, Focks A, Marinovich M, Millet M, Pelkonen O, Pieper S, Tiktak A, Topping C, Widenfalk A, Wilks M, Wolterink G, Rundlöf M, Ippolito A, Linguadoca A, Martino L, Panzarea M, Terron A, Aldrich A. Statement on the active substance acetamiprid. EFSA J. 2022 Jan 24;20(1):e07031. doi: 10.2903/j.efsa.2022.7031.

EFSA Panel on Plant Protection Products and their Residues (PPR); Hernandez Jerez A, Adriaanse P, Berny P, Coja T, Duquesne S, Focks A, Marinovich M, Millet M, Pelkonen O, Pieper S, Tiktak A, Topping C, Widenfalk A, Wilks M, Wolterink G, Rundlöf M, Ippolito A, Linguadoca A, Martino L, Panzarea M, Terron A, Aldrich A. Statement on the active substance flupyradifurone. EFSA J. 2022 Jan 24;20(1):e07030. doi: 10.2903/j.efsa.2022.7030.

Fu Y, Luechtefeld T, Karmaus A, Hartung T. The use of artificial intelligence and big data for the safety evaluation of US food-relevant chemicals. In: Knowles ME, Anelich L, Boobis A and Popping B (eds). Present knowledge in food safety – a risk-based approach throughout the food chain, Elsevier, 575-588.

Farhat N, Tsaioun K, Saunders-Hastings P, Morgan RL, Ramoju S, Hartung T, Krewski D. Systematic review in evidence-based risk assessment. ALTEX 39:463–479. doi: 10.14573/altex.2004111.

Forcella M, Lau P, Fabbri M, Fusi P, Oldani M, Melchioretto P, Gribaldo L, and Urani C. Is Cadmium Toxicity Tissue-Specific? Toxicogenomics Studies Reveal Common and Specific Pathways in Pulmonary, Hepatic, and Neuronal Cell Models. Int J Mol Sci 2022 23(3), 1-20 https://doi.org/10.3390/ijms23031768

Hartung T, Krewski D. Editorial: Special Issue Development of an Evidence-Based Risk Assessment Framework. ALTEX 39:442. doi: doi:10.14573/altex.22S2.

Hartung T, Tsatsakis AM. Safety Science in the 21st Century – a Scientific Revolution in its Making. In: Tsatsakis AM (ed). Toxicological Risk Assessment and Multi-System Health Impacts from Exposure. p. 51-59. Elsevier, 2022.

Hartung T. Replacing Animal Testing: How and When? Frontier for Young Minds 2022, 10:959496. doi: 10.3389/frym.2022.959496.

Hoffmann S, Aiassa E, Angrish M, Beausoleil C, Bois FY, Ciccolallo L, Craig PS, de Vries RBM, Dorne JLCM, Druwe IL, Edwards SW, Eskes C, Georgiadis M, Hartung T, Kienzler A, Kristjansson EA, Lam J, Martino L, Meek B, Morgan RL, Munoz-Guajardo I, Noyes PD, Parmelli E, Piersma A, Rooney A, Sena E, Sullivan K, Tarazona J, Terron A, Thayer K, Turner J, Verbeek J, Verloo D, Vinken M, Watford S, Whaley P, Wikoff D, Willett K, Tsaioun K. Application of evidence-based methods to construct mechanism-driven chemical assessment frameworks, ALTEX 2022, 39:499–518. doi: 10.14573/altex.2202141.

Huang Q, Tang B, Romero JC, Yang Y, Elsayed SK, Pahapale G, Lee T-J, Pantoja IEM, Han F, Berlinicke C, Xiang T, Solazzo M, Hartung T, Qin Z, Caffo BS, Smirnova L, Gracias DH. Shell Microelectrode Arrays (MEAs) for brain organoids. Science Advances 2022, 8:eabq5031. Doi: 10.1126/sciadv.abq5031.

Hutt A, Gruning A, Hansen A, Hartung T, Robeva R. Editorial on Machine Learning in Natural Complex Systems. Frontiers in Applied Mathematics and Statistics, Front. Appl. Math. Stat. 8:869999. doi: 10.3389/fams.2022.869999.

Karaulov AV, Smolyagin AI, Mikhailova IV, Stadnikov AA, Ermolina EV, Filippova YV, Kuzmicheva NV, Vlata ZK, Buha Djordjevic A, Christina, Hartung T, Hernandez AF, Tsatsakis AM. Assessment of the combined effects of chromium and benzene on the rat neuroendocrine and immune systems. Environmental Research 2022, 207:112096. doi: 10.1016/j.envres.2021.112096.

Krewski D, Saunders-Hastings P, Baan R, Barton-Maclaren T, Browne P, Chiu WA, Gwinn M, Hartung T, Kraft A, Lam J, Lewis JR, Sanaa M, Morgan RL, Paoli G, Rhomberg L, Rooney A, Sand S, Schünemann HJ, Straif K, Thayer K, Tsaioun, K. Workshop Report: Development of an Evidence-Based Risk Assessment Framework. ALTEX 2022, 39:667–693. doi: 10.14573/altex.2004041.

Lippa KA, Aristizabal-Henao JJ, Beger RD, Bowden JA, Broeckling C, Beecher C, Davis WC, Dunn WB, Flores R, Goodacre R, Gouveia GJ, Harms AC, Hartung T, Jones CM, Lewis CM, Ntai I, Percy AJ, Raftery D, Schock TB, Sun J, Theodoridis G, Tayyari F, Torta F, Ulmer CZ, Wilson I, Ubhi BK. Reference Materials for MS-based Untargeted Metabolomics and Lipidomics: A Review by the Metabolomics Quality Assurance and Quality Control Consortium (mQACC). Metabolomics 2022, 18:24. Doi: 10.1007/s11306-021-01848-6.

Maertens A, Golden E, Luechtefeld TH, Hoffmann S, Tsaioun K, Hartung T. Probabilistic Risk Assessment – the Keystone for the Future of Toxicology. ALTEX 2022, 39:3-29. doi:10.14573/altex.2201081.

Neuhaus W, Reininger-Gutmann B, Rinner B, Plasenzotti R, Wilflingseder D, De Kock J, Vanhaecke T, Rogiers V, Jirova D, Kejlova K, Knudsen LE, Nielsen RN, Kleuser B, Kral V, Thone-Reineke C, Hartung T, Pallocca G, Leist M, Hippenstiel S, Lang A, Retter I, Kramer S, Jedlicka P, Ameli K, Fritsche E, Tigges J, Buettner M, Bleich A, Baumgart N, Baumgart J, Meinhardt MW, Spanagel R, Chourbaji S, Kranzlin B, Seeger B, von Kockritz-Blickwede M, Sanchez-Morgado JM, Galligioni V, Ruiz-Perez D, Movia D, Prina-Mello A, Ahluwalia A, Chiono V, Gutleb AC, Schmit M, van Golen B, van Weereld L, Kienhuis A, van Oort E, van der Valk J, Smith A, Roszak J, Stepnik M, Sobanska Z, Olsson IAS, Franco NH, Sevastre B, Kandarova H, Capdevila S, Johansson J, Cederroth CR, Sandstrom J, Ragan I, Bubalo N, Spielmann H. The rise of 3R centres and platforms in Europe. ATLA 2022, 50:90-120.

Neuhaus W, Reininger-Gutmann B, Rinner B, Plasenzotti R, Wilflingseder D, De Kock J, Vanhaecke T, Rogiers V, Jírová D, Kejlová K, Knudsen LE, Nielsen RN, Kleuser B, Kral V, Thöne-Reineke C, Hartung T, Pallocca G, Rovida C, Leist M, Hippenstiel S, Lang A, Retter I, Krämer S, Jedlicka P, Ameli K, Fritsche E, Tigges J, Kuchovska E, Buettner M, Bleich A, Baumgart N, Baumgart J, Meinhardt MW, Spanagel R, Chourbaji S, Kränzlin B, Seeger B, von Köckritz-Blickwede M, Sánchez-Morgado JM, Galligioni V, Ruiz-Pérez D, Movia D, Prina-Mello D, Ahluwalia A, Chiono V, Gutleb AC, Schmit M, van Golen B, van Weereld L, Kienhuis A, van Oort E, van der Valk J, Smith A, Roszak J, Stępnik M, Sobańska Z, Reszka E, Olsson IAS, Franco NH, Sevastre B, Kandarova H, Capdevila S, Johansson J, Svensk E, Cederroth CR, Sandström J, Ragan I, Bubalo N, Kurreck J, Spielmann H. The current status and work of 3R centres and platforms in Europe. ATLA – Alternatives to Laboratory Animals 2022, 50:381-413.

Pamies D, Wiersma D, Katt ME, Zhong L, Burtscher J, Harris G, Smirnova L, Searson PC, Hartung T, Hogberg H. Human organotypic brain model as a tool to study chemical-induced dopaminergic neuronal toxicity. Neurobiology of Disease 2022, 169:105719. doi: 10.1016/j.nbd.2022.105719.

Pamies D, Leist M, Coecke S, Bowe G, Allen D, Gstraunthaler G, Bal-Price A, Pistollato F, DeVries R, Hogberg HT, Hartung T, Stacey G. Guidance Document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0). ALTEX, 2022, 39:30–70. doi: 10.14573/altex.2111011.

Sillé FCM, McCormack M, Hartung T. The Exposome applied: a step toward defining the totality of environmental exposures in asthma. American Journal of Respiratory and Critical Care Medicine 2022, 206:1187-1188. doi: 10.1164/rccm.202207-1430ED

Smirnova L, Hartung T. Neuronal cultures playing Pong – first steps toward advanced screening and biological computing. Neuron 2022, 110:3855-3856, doi: 10.1016/j.neuron.2022.11.010.

Steimberg N, Alloisio S, Vincentini O, Caloni F, Baderna D, Dellambra E, Delle Monache F, Sbrana T, Scaglione S, Letasiova S, Cattaneo A, Ludovico A, Hartung T, Colacci A, Markus J, Rescigno F, Piccapane F, Occhetta P, Scagnoli F. CellTox Days 2022 – Inside the barriers: In vitro models and their applications. ALTEX 2023, 40:160-164. doi: 10.14573/altex.2211101

von Aulock S, Busquet F, Locke P, Herrmann K, Hartung T. Engagement of scientists with the public and policymakers to promote alternative methods. ALTEX 2022, 39:543–559. doi: 10.14573/altex.2209261.

Benoit L., Jornod F., Zgheib E., Tomkiewicz C., Koual M., Coustillet T., Barouki R., Audouze K., Vinken M., Coumoul X. (2022) “Adverse outcome pathway from activation of the AhR to breast cancer-related death.” Environment International 165: 107323 (impact factor₂₀₂₂: 11.8). PMID: 35660951, DOI: 10.1016/j.envint.2022.107323

Clerbaux L.-A., Amigó N., Amorim M.J., Bal-Price A., Batista Leite S., Beronius A., Bezemer G.F.G., Bostroem A.-C., Carusi A., Coecke S., Concha R., Daskalopoulos E.P., Debernardi F., Edrosa E., Edwards S.W., Filipovska J., Garcia-Reyero N., Gavins F.N.E., Halappanavar S., Hargreaves A.J., Hogberg H., Huynh M.T., Jacobson D., Josephs-Spaulding J., Kim Y.J., Kong H.J., Krebs C.E., Lam A., Landesmann B., Racanelli Layton A., Lee Y.O., Macmillan D.S., Mantovani A., Margiotta-Casaluci L., Martens M., Masereeuw R., Mayasich S.A., Mei L.M., Mortensen H., Munoz Pineiro A., Nymark P., Ohayon E., Ojasi J.M., Paini A., Parissis N., Parvatam S., Pistollato F., Sachana M., Birkelund Sørli J., Sullivan K.M., Sund J., Tanabe S., Tsaioun K., Vinken M., Viviani L., Waspe J., Willett C., Wittwehr C. (2022) “COVID-19 through adverse outcome pathways: building networks to better understand the disease: report of the 3rd CIAO AOP design workshop.” ALTEX 39: 322-335 (impact factor₂₀₂₂: 5.6). PMID: 35032963, PMCID: PMC10069302, DOI: 10.14573/altex.2112161

Daston G.P., Mahony C., Thomas R.S., Vinken M. (2022) “Assessing safety without animal testing: the road ahead.” Toxicological Sciences 187- 214-218 (impact factor₂₀₂₂: 3.8). PMID: 35357465, DOI: 10.1093/toxsci/kfac039

Hoffmann S., Aiassa E., Angrish M., Beauso leil C., Bois F.Y., Ciccolallo L., Craig P.S., de Vries R.M.B., Dorne J.L.C.M. , Druwe I.L., Edwards S.W., Eskes C., Georgiadis M., Hartung T., Kienzler A., Kristjansson E.A., Lam J., Martino L., Meek B., Morgan R.L., Munoz-Guajardo I., Noyes P.D., Parmelli E., Piersma A., Rooney A., Sena E., Sullivan K., Tarazona J., Terron A., Thayer K., Turner J., Verbeek J., Verloo D., Vinken M., Watford S., Whaley P., Wikoff D., Willett K., Tsaioun K. (2022) “Application of evidence-based methods to construct mechanism-driven chemical assessment frameworks.” ALTEX 39: 499-518 (impact factor₂₀₂₂: 5.6). PMID: 35258090, PMCID: PMC9466297, DOI: 10.14573/altex.2202141

Masseroni A, Rizzi C, Urani C., Villa, S. Nanoplastics: Status and Knowledge gaps in the Finalization of Environmental Risk Assessments. TOXICS 2022 10(5), 1-23 https://doi.org/10.3390/toxics10050270

Mihajlovic M., Vinken M. (2022) “Mitochondria as target of hepatotoxicity and drug-induced liver injury: molecular mechanisms and detection methods.” International Journal of Molecular Sciences 23: 3315 (impact factor₂₀₂₂: 5.6). PMID: 35328737, PMCID: PMC8951158, DOI: 10.3390/ijms23063315

Sonawane A., Vadloori B., Poosala S., Kandarova H., Kulkarni M., Olayanju A., Dey T., Saxena U., Smirnova L., Kanda Y., Reddy J., Dravida S., Biswas S., Vinken M., Gettayacamin M., Ahluwalia A., Mondini F., Bhattacharya S., Kulkarni P., Jacobsen K., Vangala S., Millas A. (2022) “Advances in animal models and cutting-edge research in alternatives: proceedings of the second international conference on 3Rs research and progress, Hyderabad, 2021.” ATLA 50: 156-171 (impact factor₂₀₂₂: 2.7). PMID: 35410493, DOI: 10.1177/02611929221089216

Vinken M. (2022) “Toxic talk: pannexin1 channel communication as an emerging mechanism of toxicity.” Toxicology 478: 153295 (impact factor₂₀₂₂: 4.5). PMID: 35998787, DOI: 10.1016/j.tox.2022.153295

Hummer J, Birngruber T, Sinner F, Page L, Toner F, Roper CS, Moore, DJ, Baker MB, and Boncheva Bettex M (2022). Optimization of topical formulations using a combination of in vitro methods to quantify the transdermal passive diffusion of drugs. International Journal of Pharmaceutics. https://doi.org/10.1016/j.ijpharm.2022.121737.

CALONI, Francesca – INTROZZI, Olga – CAZZANIGA, Alessandra – BUZANSKA, Leonora – GUTLEB, Arno C. – KANDAROVA, Helena – CERIOTTI, Laura – RANALDI, Giulia – RASHIDI, Hassan – WILFLINGSEDER, Doris. Third Virtual Summer School 3Rs for ONE Science: Alternative methods: From complexity to predictivity : meeting report. In ALTEX : Alternatives to Animal Experimentation, 2022, vol. 39, no. 4, p. 710-711. https://doi.org/10.14573/altex.2207112

Kluxen FM, Roper CS, Jensen SM and Koenig CM (2022). Characterizing local acute irritation properties of captan and folpet with new approach methods. Applied In Vitro Toxicology 8 (3); 83-101. https://doi.org/10.1089/aivt.2022.0004

NEUHAUS, Winfried – REININGER-GUTMANN, Birgit – RINNER, Beate – WILFLINGSEDER, Doris – JÍROVÁ, Dagmar – KEJLOVÁ, Kristína – KANDAROVA, Helena – SPIELMANN, Horst. The Current Status and Work of Three Rs Centres and Platforms in Europe. In Alternatives to Laboratory Animals (ATLA) : Fund for the Replacement of Animals in Medical Experiments, 2022, vol. 50, no. 6, p. 381-413. ISSN 0261-1929. https://doi.org/10.1177/02611929221140909

NEUHAUS, Winfried – REININGER-GUTMANN, Birgit – RINNER, Beate – PLASENZOTTI, Roberto – WILFLINGSEDER, Doris – KANDAROVA, Helena. The rise of three Rs centres and platforms in Europe. In Alternatives to Laboratory Animals (ATLA), 2022, vol. 50, no. 2, p. 90-120. ISSN 0261-1929. https://doi.org/10.1177/02611929221099165

Roper CS, Hargrove MM, Sullivan K and Wolf D (2022). Case study on the use of an Integrated Approach for Testing and Assessment (IATA) for New Approach Methodology (NAM) for refining inhalation risk assessment from point of contact toxicity of the pesticide, chlorothalonil. ENV/CBC/MONO(2022)31. OECD Environment, Health and Safety Publications Series on Testing and Assessment No. 367. https://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/cbc/mono(2022)31&doclanguage=en

SEIDLE, Troy – KANĎÁROVÁ, Helena – HARTUNG, Thomas – LEIST, Marcel – NEUHAUS, Winfried – SPIELMANN, Horst – ROVIDA, Costanza. Open letter: Selection of a new Executive Director of the European Chemicals Agency (ECHA) provides an opportunity for the EU to lead in the field of chemicals management and implementation of innovative science = Selection of a new Executive Director of the European Chemicals Agency (ECHA) provides an opportunity for the EU to lead in the field of chemicals management and implementation of innovative science. In ALTEX : Alternatives to Animal Experimentation, 2022, vol. 39, no. 1, p. 149-150. ISSN 1868-596X. https://doi.org/10.14573/altex.2110271

SONAWANE, Akshay – VADLOORI, Bharadwaja – POOSALA, Suresh – KANĎÁROVÁ, Helena – KULKARNI, Mandar – OLAYANJU, Adedamola – DEY, Tuli – SAXENA, Uday – SMIRNOVA, Lena – KANDA, Yasunari – REDDY, Jaganmohan – DRAVIDA, Subhadra – BISWAS, Saikat – VINKEN, Mathieu – GETTAYACAMIN, Montip – AHLUWALIA, Arti – MONDINI, Franco – BHATTACHARYA, Somshuvra – KULKARNI, Pushkar – JACOBSEN, Kirsten Rosenmay – VANGALA, Subrahmanyam – MILLÁS, Ana Luiza. Advances in animal models and cutting-edge research in alternatives: Proceedings of the Second nternational Conference on 3Rs research and progress, Hyderabad, 2021. In Alternatives to Laboratory Animals (ATLA), 2022, vol. 50, no. 2, p. 156-171. ISSN 0261-1929. https://doi.org/10.1177/02611929221089216

2021

Abass K, Pelkonen O, Rautio A. Chloro-s-triazines-toxicokinetic, Toxicodynamic, Human Exposure, and Regulatory Considerations. Curr Drug Metab. 2021;22(8):645-656. doi: 10.2174/1389200222666210701164945. Review.

Anderson WA, Bosak A, Hogberg HT, Hartung T, Moore MJ. Advances in 3D neuronal microphysiological systems: towards a functional nervous system on a chip. In Vitro Cellular & Developmental Biology – Animal 2021, 57:191–206 Doi: 10.1007/s11626-020-00532-8

Aschner M, Paoliello MMB, Tsatsakis A, Bowman AB, Dorea JG, Hartung T, Domingo JL, Barbosa F J. Social Injustice in Environmental Health: A Call for Fortitude. Environmental Research 2021, 194: 110675. doi: 10.1016/j.envres.2020.110675

Barile FA, Berry SC, Blaauboer B, Boobis A, Bolt HM, Borgert C, Dekant W, Dietrich D, Domingo JL, Galli CL, Gori GB, Greim H, Hengstler JG, Heslop-Harrison P, Kacew S, Marquardt H, Mally A, Pelkonen O, Savolainen K, Testai E, Tsatsakis A, Vermeulen NP. The EU chemicals strategy for sustainability: in support of the BfR position. Arch Toxicol. 2021 Sep;95(9):3133-3136. doi: 10.1007/s00204-021-03125-w.

Barile FA, Berry SC, Blaauboer B, Boobis A, Bolt H, Borgert CJ, Dekant W, Dietrich D, Domingo JL, Gori GB, Greim H, Hengstler J, Kacew S, Marquardt H, Pelkonen O, Savolainen K, Heslop-Harrison P, Tsatsakis A, Vermeulen NP. Critique of the “Comment” etitled “Pyrethroid exposure: Not so harmless after all” by Demeneix et al. (2020) published in the lancet diabetes endocrinology. Toxicol Lett. 2021 Apr 1;340:1-3. doi: 10.1016/j.toxlet.2020.12.020.

Bovio F, Sciandrone B, Urani C, Fusi P, Forcella M, Regonesi M. Superoxide dismutase 1 (SOD1) and cadmium: a three models’ approach to the comprehension of its neurotoxic effects. Biomed Sci Eng, 2021 4(s1) https://doi.org/10.4081/bse.157

Bovio F, Sciandrone B, Urani C, Fusi P, Forcella M, Regonesi M. Superoxide dismutase 1 (SOD1) and cadmium: A three models’ approach to the comprehension of its neurotoxic effects. Neurotoxicol 2021 84(May 2021), 125-135 https://doi.org/10.1016/j.neuro.2021.03.007

Bovio F, Melchioretto P, Forcella M, Fusi P, Urani C. Cadmium promotes glycolysis upregulation and glutamine dependency in human neuronal cells. Neurochem Int 2021 149(October 2021) https://doi.org/10.1016/j.neuint.2021.105144

EFSA Panel on Plant Protection Products and their Residues (EFSA PPR Panel); Hernandez-Jerez AF, Adriaanse P, Aldrich A, Berny P, Coja T, Duquesne S, Focks A, Marinovich M, Millet M, Pelkonen O, Pieper S, Tiktak A, Topping CJ, Widenfalk A, Wilks M, Wolterink G, Gundert-Remy U, Louisse J, Rudaz S, Testai E, Lostia A, Dorne JL, Parra Morte JM. Scientific Opinion of the Scientific Panel on Plant Protection Products and their Residues (PPR Panel) on testing and interpretation of comparative in vitro metabolism studies. EFSA J. 2021 Dec 23;19(12):e06970. doi: 10.2903/j.efsa.2021.6970.

EFSA Panel of the Plant Protection Products and their Residues (PPR); Hernandez-Jerez A, Adriaanse P, Aldrich A, Berny P, Coja T, Duquesne S, Focks A, Marina M, Millet M, Pelkonen O, Tiktak A, Topping C, Widenfalk A, Wilks M, Wolterink G, Conrad A, Pieper S. Statement of the PPR Panel on a framework for conducting the environmental exposure and risk assessment for transition metals when used as active substances in plant protection products (PPP). EFSA J. 2021 Mar 29;19(3):e06498. doi: 10.2903/j.efsa.2021.6498.

Oldani M, Milani C., Gaboardi, F., Manzoni, M., Villa, A., Stefanini, F., Melchioretto P, Monti E, Forcella M, Urani C, Fusi P. Cadmium elicits alterations in mitochondrial morphology and functionality in C3H10T1/2Cl8 mouse fibroblast cell. Biomed Sci Eng. 2021 4(s1) https://doi.org/10.4081/bse.185

Bertanza G, Boniotti J, Ceretti E, Feretti D, Mazzoleni G, Menghini M, Pedrazzani R, Steimberg N, Urani C, Viola GCV, Zerbini I, Ziliani E. Environmental footprint of wastewater treatment: A step forward in the use of toxicological tools. Int. J. Environ. Res. Public Health 2021 18(13) https://doi.org/10.3390/ijerph18136827

Brown J, Clippinger AJ, Goode J, Ghosh M, Briglia C, Casey W, Coleman K, Fritsch A, Hartung T, Maouyo D, Muller T, Reich J, Robert L, Roeder R, Sanchez G, Sawyer A, Solati S, Tirumalai R, Zwislr W, Allen D. Using the Monocyte Activation Test as a Stand-alone Release Test for Medical Devices: A Workshop Report. ALTEX 2021, 38;151-156.

Calina D, Hartung T, Mardare I, Mitroi M, Poulas K, Tsatsakis A, Rogoveanu I, DoceaOA. COVID-19 pandemic and alcohol consumption: impacts and interconnections. Toxicology Reports 2021, 8:529-535. Doi: 10.1016/j.toxrep.2021.03.

Chesnut M, Hartung T, Hogberg HT, Pamies D. Human oligodendrocytes and myelin in vitro to evaluate developmental neurotoxicity. International Journal of Molecular Sciences 2021, 22:7929. doi: 10.3390/ijms22157929.

Chesnut M, Paschoud H, Repond C, Smirnova L, Hartung T, Zurich M-G, Hogberg HT, Pamies D. Human 3D iPSC-derived brain model to study chemical-induced myelin disruption. International Journal of Molecular Sciences, 22:9473. doi: 10.3390/ijms22179473.

de Vries RBM, Angrish M, Browne P, Brozek J, Rooney AA, Wikoff DS, Whaley P, Edwards SW, Morgan RL, Druwe IL, Hoffmann S, Hartung T, Thayer K, Avey MT, Beverly BEJ, Falavigna M, Gibbons C, Goyak K, Kraft A, Klugar M, Klugarova J, Nampo F, Qaseem A, Sears M, Singh JA, Willett K, Yost EY, Schünemann H, Tsaioun K. Applying evidence-based methods to the development and use of adverse outcome pathways construct mechanistic frameworks for the development and use of non-animal toxicity tests. ALTEX 2021, 38:336-347. doi:10.14573/altex.2101211

Farsalinos K, Poulas K, Kouretas D, Vantarakis A, Leotsinidis M, Kouvelas D, Docea AO, Kostoff R, Gerotziafas GT, Antoniou MN, Polosa R, Barbouni A, Yiakoumaki V, Giannouchos TV, Bagos PG, Lazopoulos G, Izotov BN, Tutelyan VA, Aschner M, Hartung T, Wallace HM, Carvalho F, Domingo JL, Tsatsakis A. Improved strategies to counter the COVID-19 pandemic: Lockdowns vs. Primary and Community Healthcare. Toxicology Reports 2021, 8:1-9. Doi: 10.1016/j.toxrep.2020.12.001

Golden E, Macmillan DS, Dameron G, Kern P, Hartung T, Maertens A. Evaluation of the global performance of eight in silico skin sensitization models using human data. ALTEX 2021, 38:33-48. doi: 10.14573/altex.1911261

Golden E, Maertens M, Hartung T, Maertens A. Mapping Respiratory Sensitization: How Useful Are Our Current Tools? Chemical Research in Toxicology 2021, 34:473-482. doi: 10.1021/acs.chemrestox.0c00320.

Hakkola J, Hukkanen J, Turpeinen M, Pelkonen O. Inhibition and induction of CYP enzymes in humans: an update. Arch Toxicol. 2020 Nov;94(11):3671-3722. doi: 10.1007/s00204-020-02936-7. Review.

Hartung T. Pyrogen testing revisited on occasion of the 25th anniversary of the whole blood test. ALTEX 2021, 38:3-19. doi: 10.14573/altex.2101051.

Hartung T. Evidence integration in the era of information flooding – the advent of the comprehensive review. Frontiers in Public Health 2021, 9:763828. doi: 10.3389/fpubh.2021.763828.

Hartung T, Tsatsakis AM. The state of the Scientific Revolution in Toxicology. ALTEX 2021, 38:379-386. doi:10.14573/altex.2106101.

Hoffmann S, Margliani B, Akgün-Ölmez SG, Ireland D, Cruz R, Busquet F, Flick B, Lalu M, Ghandakly EC, de Vries R, Witters H, Wright RA, Ölmez M, Willett C, Hartung T, Stephens ML, Tsaioun K. A systematic review to compare chemical hazard predictions of the zebrafish embryo test with mammalian prenatal developmental toxicity. Toxicological Sciences 2021, 183:14–35. doi: 10.1093/toxsci/kfab072

Hogberg HT, de Cássia da Silveira e Sá R, Kleensang A, Bouhifd M, Cemiloglu Ulker O, Smirnova L, Behl M, Maertens A, Zhao L, Hartung T. Organophosphorus flame retardants are developmental neurotoxicants in a rat primary BrainSphere in vitro model, Arch Toxicol 2021, 95:207-228, doi: 10.1007/s00204-020-02903-2

Kang I, Smirnova L, Kuhn JH, Hogberg HT, Kleinstreuer NC, Hartung T. COVID-19 – prime time for microphysiological systems, as illustrated for the brain, ALTEX 2021, 38:535-549. doi: 10.14573/altex.2110131

Knight J, Rovida C, Kreiling R, Zhu C, Knudsen M, Hartung T. Continuing animal tests on cosmetic ingredients for REACH in the EU. ALTEX, 2021 38:653-668. doi: 10.14573/altex.2104221

Locke PA, Singer M, Hartung T. The Humane Research and Testing Act: Advancing Science by Creating a New Center for Alternatives at the US National Institutes of Health. ALTEX 2021, 38:678-680. doi: 10.14573/altex.2106031

Maertens A, Golden E, Hartung T. Avoiding Regrettable Substitutions: Green Toxicology for Sustainable Chemistry. ACS Sustainable Chemistry & Engineering 2021, 9, 23, 7749–7758

Mansouri K, Karmaus A, Fitzpatrick J, Patlewicz G, Pradeep P, Alberga D, Alepee N, Allen T, Allen D, Alves VM, Andrade CH, Auernhammer TT, Ballabio D, Bell S, Benfenati E, Bhattacharya S, Bastos JV, Boyd S, Brown JB, Capuzzi SJ, Chushak Y, Ciallella H, Clark AM, Consonni V, Daga PR, Ekins S, Farag S, Fedorov M, Fourches D, Gadaleta D, Gao F, Gearhart JM, Goh G, Goodman JM, Grisoni F, Grulke CM, Hartung T, Hirn M, Karpov P, Korotcov A, Lavado GJ, Lawless M, Li X, Luechtefeld T, Lunghini F, Mangiatordi GF, Marcou G, Marsh D, Martin T, Mauri A, Muratov EN, Myatt GJ, Nguyen D-T, Nicolotti O, Note R, Pande P, Parks AK, Peryea T, Polash A, Rallo R, Roncaglioni A, Rowlands C, Ruiz P, Russo D, Sayed A, Sayre R, Sheils T, Siegel C, Silva AC, Simeonov A, Sosnin S, Southall N, Strickland J, Tang Y, Teppen B, Tetko IV, Thomas D, Tkachenko V, Todeschini R, Toma C, Tripodi I, Trisciuzzi D, Tropsha A, Varnek A, Vukovic K, Wang Z, Wang L, Waters KM, Wedlake AJ, Wijeyesakere SJ, Wilson D, Xiao Z, Yang H, Zahoranszky-Kohalmi G, Zakharov AV, Zhang FF, Zhang Z, Zhao T, Zhu H, Zorn KM, Casey W, Kleinstreuer NC. CATMoS: Collaborative Acute Toxicity Modeling Suite. Environmental Health Perspectives 2021, 129:47013. doi: 10.1289/EHP8495

Modafferi S, Zhong X, Kleensang A, Murata Y, Fagiani F, Pamies D, Hogberg HT, Calabrese V, Lachman H, Hartung T, Smirnova L. Gene–environment interactions in developmental neurotoxicity: a case study of synergy between chlorpyrifos and CHD8 knockout in human BrainSpheres. Environmental Health Perspectives 2021, 129:77001. Doi: 10.1289/EHP8580

Roth A, MPS-WS Berlin 2019 [Marx U, Vilén L, Ewart L, Griffith LG, Hartung T, Ingber DE, Mendrick DL, Steger-Hartmann T, Tagle DA]. Human microphysiological systems for drug development. Science 2021, 373:1304-1306

Sarigiannis DA, Hartung T, Karakitsios SP. The exposome—a new paradigm for non-animal toxicology and integrated risk assessment. In: Tsatsakis AM. Toxicological Risk Assessment and Multi-System Health Impacts from Exposure. Elsevier, Academic Press, London, 2021, 23-30.

Spoladore J, Lopes IG, Bachinski RF, Negherbon JP, Hartung T, Granjeiro JM, Alves GG. Standardized pyrogen testing of health products with bacterial endotoxin tests (BET) as a substitute for rabbit pyrogen testing (RPT): a scoping review. Toxicology In Vitro, 2021, 74, 105160. Available online: doi: 10.1016/j.tiv.2021.105160

Tran V, Kim R, Maertens M, Hartung T, Maertens A. Similarities and differences in gene expression networks between the breast cancer cell line MCF-7 and invasive human breast cancer tissues. Frontiers in Artificial Intelligence, section Medicine and Public Health 2021, 4:674370. doi: 10.3389/frai.2021.674370

Vinken M, Benfenati E, Busquet F, Castell J, Clevert D-A, de Kok T, Dirven H, Fritsche E, Geris L, Gozalbes R, Hartung T, Jennen D, Jover R, Kandarova H, Kramer N, Krul C, Luechtefeld T, Masereeuw R, Roggen E, Schaller S, Vanhaecke T, Yang C,, Piersma AH. Safer chemicals using less animals: kick-off of the European ONTOX project. Toxicology 2021, 458, 152846, doi: 10.1016/j.tox.2021.152846

Wang T, Liu H, Itoh K, Oh S, Zhao L, Murata D, Sesaki H, Hartung T, Na CH, Wang J. C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly. Cell Metabolism 2021, 33:531-546.e9. doi: 10.1016/j.cmet.2021.01.005

Arnesdotter E., Rogiers V., Vanhaecke T., Vinken M. (2021) “An overview of current practices for regulatory risk assessment with lessons learnt from cosmetics in the European Union.” Critical Reviews in Toxicology 51: 395-417(impact factor₂₀₂₁: 6.184). PMID: 34352182, DOI: 10.1080/10408444.2021.1931027

Arnesdotter E., Spinu N., Firman J., Ebbrell D., Cronin M.T.D., Vanhaecke T., Vinken M. (2021) “Derivation, characterisation and analysis of an adverse outcome pathway network for human hepatotoxicity.” Toxicology 459: 152856 (impact factor₂₀₂₁: 4.571). PMID: 34252478, DOI: 10.1016/j.tox.2021.152856

Firman J.W., Pestana C.B., Rathman J.F., Vinken M., Yang C., Cronin M.T.D. (2021) “A robust, mechanistically based in silico structural profiler for hepatic cholestasis.” Chemical Research In Toxicology 34: 641-655 (impact factor₂₀₂₁: 3.973). PMID: 33314907, DOI: 10.1021/acs.chemrestox.0c00465

Gijbels E., Devisscher L., Vinken M. (2021) “Testing in vitro tools for the prediction of cholestatic liver injury induced by non-pharmaceutical chemicals.” Food and Chemical Toxicology 152: 112165 (impact factor₂₀₂₁: 5.572). PMID: 33819548, DOI: 10.1016/j.fct.2021.112165

Gijbels E., Devisscher L., Vinken M. (2021) “Dataset on transcriptomic profiling of cholestatic liver injury induced by food additives and a cosmetic ingredient.” Data in Brief 38: 107373 (no impact factor₂₀₂₁). PMID: 34589561, PMCID: PMC8461341, DOI: 10.1016/j.dib.2021.107373

Leroy K., Pieters A., Cooreman A., Van Campenhout R. Cogliati B., Vinken M. (2021) “Connexin-based channel activity is not specifically altered by hepatocarcinogenic chemicals.” International Journal of Molecular Sciences 22: 17424 (impact factor₂₀₂₁: 6.208). PMID: 34769157, PMCID: PMC8584159, DOI: 10.3390/ijms222111724

Leroy K., Silva Costa C.J., Pieters A., dos Santos Rodrigues B., Van Campenhout R., Cooreman A., Tabernilla A., Cogliati B., Vinken M. (2021) “Expression and functionality of connexin-based channels in human liver cancer cell lines.” International Journal of Molecular Sciences 22: 12187 (impact factor₂₀₂₁: 6.208). PMID: 34830068, PMCID: PMC8623148, DOI: 10.3390/ijms222212187

Oorts M., Van Brantegem P., Deferm N., Chatterjee S., Dreesen E., Cooreman A., Vinken M., Richert L., Annaert P. (2021) “Bosentan alters endo- and exogenous bile salt disposition in sandwich-cultured human hepatocytes.” Journal of Pharmacology and Experimental Therapeutics 379: 20-32(impact factor₂₀₂₁: 4.441). PMID: 34349015, DOI: 10.1124/jpet.121.000695

Oldani M, Villa A, Manzoni M, Melchioretto P, Parenti P, Monti E, Fusi P, Forcella M, Urani C. Insights into Cadmium‐Induced Carcinogenesis through an In Vitro Study Using C3H10T1/2Cl8 Cells: The Multifaceted Role of Mitochondria. Int. J. Mol. Sci. 2021 22(19), 1-19 https://doi.org/10.3390/ijms221910837

Pieters A., Gijbels E., Cogliati B., Annaert P., Devisscher L., Vinken M. (2021) “Biomarkers of cholestasis.” Biomarkers in Medicine 15: 437-454 (impact factor₂₀₂₁: 2.498). PMID: 33709780, DOI: 10.2217/bmm-2020-0691

Tabernilla A., dos Santos Rodrigues B., Pieters A., Caufriez A., Leroy K., Van Campenhout R., Cooreman A., Gomes A.R., Arnesdotter E., Gijbels E., Vinken M. (2021) “In vitro liver toxicity testing of chemicals: a pragmatic approach” International Journal of Molecular Sciences 22: 5038 (impact factor₂₀₂₁: 6.208). PMID: 34068678, PMCID: PMC8126138, DOI: 10.3390/ijms22095038

Vilas-Boas V., Vinken M. (2021) “Hepatotoxicity induced by nanomaterials: mechanisms and in vitro models.” Archives of Toxicology 95: 27-52 (impact factor₂₀₂₁: 6.168).

Vilas-Boas V., Gijbels E., Leroy K., Pieters A., Baze A., Parmentier C., Vinken M. (2021) “Primary human hepatocyte spheroids as tools to study the hepatotoxic potential of non-pharmaceutical chemicals.” International Journal of Molecular Sciences 22: 11005 (impact factor₂₀₂₁: 6.208). PMID: 34681664, PMCID: PMC8537720, DOI: 10.3390/ijms222011005

Vinken M. (2021) “COVID-19 and the liver: an adverse outcome pathway perspective.” Toxicology 455: 152765 (impact factor₂₀₂₁: 4.571). PMID: 33771662, PMCID: PMC7986318. DOI: 10.1016/j.tox.2021.152765

Vinken M., Benfenati E., Busquet E., Castell J., Clevert D.-A., de Kok T., Dirven H., Fritsche E., Geris L., Gozalbes R., Hartung T., Jennen D., Jover R., Kandarova H., Kramer N., Krul C., Luechtefeld T., Masereeuw R., Roggen E., Schaller S., Vanhaecke T., Yang C., Piersma A.H. (2021) “Safer chemicals using less animals: kick-off of the European ONTOX project.” Toxicology 458: 152846 (impact factor₂₀₂₁: 4.571). PMID: 34216698, DOI: 10.1016/j.tox.2021.152846

CALONI, Francesca – CAZZANIGA, Alessandra – COCCINI, Teresa – GUTLEB, Arno C. – KANDAROVA, Helena – CERIOTTI, Laura – RASHIDI, Hassan – SAMBUY, Yula – VON AULOCK, Sonja – WILFLINGSEDER, Doris. Second Virtual Summer School: Alternative Methods in Science: Towards Model Complexity : meeting report. In ALTEX : Alternatives to Animal Experimentation, 2021, vol. 38, no. 3, p. 510-512. https://doi.org/10.14573/altex.2106221

JANOUŠEK, Stanislav – VLKOVÁ, Alena** – JÍROVÁ, Gabriela – KEJLOVÁ, Kristína – KRSEK, Daniel – JÍROVÁ, Dagmar – KANĎÁROVÁ, Helena – WITTLINGEROVÁ, Zdenka – HEINONEN, Tuula – MANNERSTROM, Marika – MALÝ, Marek. Qualitative and Quantitative Analysis of Certain Aspects of the Cytotoxic and Genotoxic Hazard of Hospital Wastewaters by Using a Range of In Vitro Assays. In ATLA-Alternatives to Laboratory Animals : Fund for the Replacement of Animals in Medical Experiments, 2021, vol. 49, no. 1-2, p. 33-48. ISSN 0261-1929. https://doi.org/10.1177/02611929211004956

Kandarova H . Alternative methods to animal experimentation and their role in modern toxicology. In Toxicological Risk Assessment and Multi-System Health Impacts from Exposure. – Elsevier Inc., Academic Press, 2021, part 1, chapter 2, P. 13-22. ISBN 978-0-323-85215-9. https://doi.org/10.1016/B978-0-323-85215-9.00047-7

Kandarova H, Hayden P (2021) Standardised Reconstructed Skin Models in Toxicology and Pharmacology: State of the Art and Future Development. In Handbook of Experimental Pharmacology. – Springer Nature, 2021, 2021, vol. 265, p. 57-71. ISSN 0171-2004. https://doi.org/10.1007/164_2020_417

Laperrouze J. (2021) How Non-Animal Testing Can Reshape Health and Environment Policies. Vox Europ link

Lauschke K, Treschow AF, Rasmussen MA, Davidsen N, Holst B, Emnéus JK, Taxvig C and Vinggaard AM (2021) Creating a human NKX2.5 reporter stem cell line for developmental toxicity testing. Arch Toxicol p.1-12 doi: 10.1007/s00204-021-03018-y

Lauschke K, Volpini L, Liu Y, Vinggaard AM, Hall VJ (2021) A comparative assessment of marker expression between cardiomyocyte differentiation of human iPSCs and the developing pig heart. Stem Cells Dev 30(7):374-385 doi: 10.1089/scd.2020.0184

Plaza A, Toner F, Harris J, Ottersbach P, Roper Cand Mahony C (2021). Support for regulatory assessment of percutaneous absorption of retronecine-type pyrrolizidine alkaloids through human skin. Support for regulatory assessment of percutaneous absorption of retronecine-type pyrrolizidine alkaloids through human skin. Planta Medica 88 (2); 144-151. https://doi.org/10.1055/a-1505-8524.

Rodríguez-Carrillo A, Rosenmai AK, Mustieles V, Couderq S, Fini J-B, Vela-Soria F, Molina-Molina JM, Ferrando-Marco P, Bonefeld-Jorgensen EC, Olea N, Vinggaard AM, Fernández MF (2021) Assessment of chemical mixtures using biomarkers of combined biological activity: Screening study with human placentas. Reprod Toxicol 100:143-154 doi: 10.1016/j.reprotox.2021.01.002

Singh B, Abdelgawad ME, Ali Z, Bailey J, Budyn E, Civita P, Clift MJD, Connelly JT, Constant S, Hittinger M, Kandarova H, Kearns VR, Kiuru T, Kostrzewski T, Kress S, Durban VM, Lehr CM, McMillan H, Metz JK, Monteban V, Movia D, Neto C, Owen C, Paasonen L, Palmer KA, Pilkington GJ, Pilkington K, Prina-Mello A, Roper C, Sheard J, Smith S, Turner JE, Roy I, Tutty MA, Velliou E, Wilkinson JM (2021). Towards more predictive, physiological and animal-free in vitro models: advances in cell and tissue Culture 2020 conference proceedings. Alternatives to Laboratory Animals 2021 Jul 6:2611929211025006. doi: 10.1177/02611929211025006. Epub ahead of print. PMID: 34225465.

Scrivani M, Forcella M, Bovio F, Bogni A, Fabbri M, Gribaldo L, Andò S, Melchioretto P, Tringali M, Fusi P, Urani C. In vitro Multi-level Approaches to Study Cadmium Neurotoxicity. Biomed Sci Eng 2021 4(s1) https://doi.org/10.4081/bse.190

Spielmann H, Kandarova H (2021). Integration of Advanced Technologies into Regulatory Toxicology. In Regulatory Toxicology. 2nd edition. Springer Nature Switzerland AG, 2021, 14 p. ISBN 978-3-030-57498-7. https://doi.org/10.1007/978-3-642-36206-4_34-2

Spielmann H, Kandarova H (2021). International Regulation of Toxicological Test Procedures. In Regulatory Toxicology. 2nd edition. Springer Nature Switzerland AG, 2021, 10 p. ISBN 978-3-030-57498-7. https://doi.org/10.1007/978-3-642-36206-4_41-2

Tesauro, M., Bruschi, M., Filippini, T., D’Alfonso, S., Mazzini, L., Corrado, L., Consonni M, Vinceti M, Fusi P, Urani C. Metal(loid)s role in the pathogenesis of amyotrophic lateral sclerosis: Environmental, epidemiological, and genetic data. Environ Res, 2021 192(January 2021), 110292-110300 https://doi.org/10.1016/j.envres.2020.110292

Weber T, Wirths F, Brakebusch N, van der Valk J (2021) Reply to comment ‘Animal welfare and ethics in the collection of fetal blood for the production of fetal bovine serum’, ALTEX 38(2): 324-326 doi: 10.14573/altex.2103191

Welch J, Wallace J, Lansley A and Roper (2021). Evaluation of the toxicity of sodium dodecyl sulphate (SDS) in the MucilAir™ human airway model in vitro. Regulatory Toxicology and Pharmacology 125; 105022. https://doi.org/10.1016/j.yrtph.2021.105022.

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