This 6-day course will be organized on 5-10 April 2020 by the European Society of Toxicology In Vitro (ESTIV) in collaboration with the Belgian Society of Toxicology and Ecotoxicology (BelTox), the Université Catholique de Louvain (UCLouvain) and MatTek In Vitro Life Science Laboratories s.r.o. The course will take place at the Woluwé campus of the UCLouvain in Brussels-Belgium. The course combines cutting-edge lectures by leading experts with highly interactive group exercises. The lectures cover a broad spectrum of subjects pertinent to regulatory, screening and investigative in vitro toxicology. The group exercises consist of real-life case studies in which the participants will apply the principles of in vitro toxicology to the risk assessment of chemical substances. Furthermore, the participants will be given the opportunity to gain hands-on experience with respect to skin and eye irritation testing in vitro as well as gastro-intestinal testing in vitro. An open-book exam will be organized at the end of the course.